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  4. Rat Caspase-1(CASP1) ELISA kit

Rat Caspase-1(CASP1) ELISA kit

  • 中文名稱：
    大鼠半胱氨酸天冬氨酸蛋白酶1(CASP1)酶聯免疫試劑盒
  • 貨號：
    CSB-EL004543RA
  • 規格：
    96T/48T
  • 價格：
    ￥3600/￥2500
  • 其他：

產品詳情

  • 產品描述：
    大鼠半胱氨酸天冬氨酸蛋白酶1(CASP1)酶聯免疫試劑盒（CSB-EL004543RA）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、細胞裂解物、組織培養上清液樣本中的CASP1含量。CASP1即胱天蛋白酶1，其在炎癥反應和細胞凋亡中扮演重要角色。研究機制方面，它可激活白細胞介素等促炎因子，引發炎癥級聯反應。對其深入研究有助于理解炎癥性疾病發病機制，為相關疾病治療提供新方向。試劑盒檢測范圍為6.25 pg/mL-400 pg/mL，研究人員可將其應用于炎癥機制研究、藥物干預效果評估或細胞模型中CASP1動態變化的監測，特別在神經免疫、感染性疾病模型構建及免疫調節劑開發等基礎研究中具有重要價值本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
  • 別名：
    Casp1 ELISA kit; Il1bc ELISA kit; Il1bceCaspase-1 ELISA kit; CASP-1 ELISA kit; EC 3.4.22.36 ELISA kit; Interleukin-1 beta convertase ELISA kit; IL-1BC ELISA kit; Interleukin-1 beta-converting enzyme ELISA kit; ICE ELISA kit; IL-1 beta-converting enzyme ELISA kit; p45) [Cleaved into: Caspase-1 subunit p20; Caspase-1 subunit p10] ELISA kit
  • 縮寫：
  • Uniprot No.：
  • 種屬：
    Rattus norvegicus (Rat)
  • 樣本類型：
    serum, plasma, cell lysates, cell culture supernates
  • 檢測范圍：
    6.25 pg/mL-400 pg/mL
  • 靈敏度：
    1.56 pg/mL
  • 反應時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領域：
    Cell Biology
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%        
    Three samples of known concentration were tested twenty times on one plate to assess.    
    Inter-assay Precision (Precision between assays): CV%<10%        
    Three samples of known concentration were tested in twenty assays to assess.      
                   
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat CASP1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.  
    　 Sample Serum(n=4)    
    1:1 Average % 91    
    Range % 88-93    
    1:2 Average % 95    
    Range % 90-102    
    1:4 Average % 97    
    Range % 94-101    
    1:8 Average % 94    
    Range % 91-98    
  • 回收率：
    The recovery of rat CASP1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.  
     
    Sample Type Average % Recovery Range    
    Serum (n=5) 90 86-94    
    EDTA plasma (n=4) 99 93-105    
                   
                   
  • 標準曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.  
     
    pg/ml OD1 OD2 Average Corrected    
    400 2.189 2.321 2.255 2.047    
    200 1.729 1.754 1.742 1.534    
    100 1.151 1.165 1.158 0.950    
    50 0.755 0.748 0.752 0.544    
    25 0.456 0.468 0.462 0.254    
    12.5 0.317 0.325 0.321 0.113    
    6.25 0.277 0.290 0.284 0.076    
    0 0.201 0.215 0.208 　    
  • 數據處理：
  • 貨期：
    3-5 working days

引用文獻

產品評價

靶點詳情

  • 功能：
    Thiol protease involved in a variety of inflammatory processes by proteolytically cleaving other proteins, such as the precursors of the inflammatory cytokines interleukin-1 beta (IL1B) and interleukin 18 (IL18) as well as the pyroptosis inducer Gasdermin-D (GSDMD), into active mature peptides. Plays a key role in cell immunity as an inflammatory response initiator: once activated through formation of an inflammasome complex, it initiates a proinflammatory response through the cleavage of the two inflammatory cytokines IL1B and IL18, releasing the mature cytokines which are involved in a variety of inflammatory processes. Cleaves a tetrapeptide after an Asp residue at position P1. Also initiates pyroptosis, a programmed lytic cell death pathway, through cleavage of GSDMD. In contrast to cleavage of interleukins IL1B and IL1B, recognition and cleavage of GSDMD is not strictly dependent on the consensus cleavage site but depends on an exosite interface on CASP1 that recognizes and binds the Gasdermin-D, C-terminal (GSDMD-CT) part. Upon inflammasome activation, during DNA virus infection but not RNA virus challenge, controls antiviral immunity through the cleavage of CGAS, rendering it inactive. In apoptotic cells, cleaves SPHK2 which is released from cells and remains enzymatically active extracellularly.
  • 亞細胞定位：
    Cytoplasm. Cell membrane.
  • 蛋白家族：
    Peptidase C14A family
  • 數據庫鏈接：


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