在线日韩日本国产亚洲丨少妇伦子伦情品无吗丨欧美性猛交xxxx免费看蜜桃丨精品人妻系列无码一区二区三区丨亚洲精品无码不卡在线播放

Your Good Partner in Biology Research

  • 中文名稱(chēng):
    大鼠自噬蛋白(BECN1)酶聯(lián)免疫試劑盒
  • 貨號(hào):
    CSB-EL002658RA
  • 規(guī)格:
    96T/48T
  • 價(jià)格:
    ¥3600/¥2500
  • 其他:

產(chǎn)品詳情

  • 產(chǎn)品描述:
    大鼠自噬蛋白(BECN1)酶聯(lián)免疫試劑盒(CSB-EL002658RA)為雙抗夾心法ELISA試劑盒,定量檢測(cè)血清、血漿、組織勻漿樣本中的BECN1含量。BECN1是重要的自噬相關(guān)靶點(diǎn)。其參與自噬起始調(diào)控,在細(xì)胞穩(wěn)態(tài)維持中發(fā)揮關(guān)鍵作用。當(dāng)下研究聚焦于其在疾病發(fā)生發(fā)展中的機(jī)制,如腫瘤的生長(zhǎng)、轉(zhuǎn)移等,有望通過(guò)調(diào)控BECN1為相關(guān)疾病治療提供新策略。試劑盒檢測(cè)范圍為0.156 ng/mL-10 ng/mL,適用于研究自噬相關(guān)疾病模型中BECN1的蛋白表達(dá)動(dòng)態(tài),如阿爾茨海默病、帕金森病等神經(jīng)系統(tǒng)疾病,或癌癥、代謝綜合征等實(shí)驗(yàn)動(dòng)物模型的機(jī)制探索。滿(mǎn)足基礎(chǔ)科研中對(duì)自噬調(diào)控機(jī)制、藥物干預(yù)效果評(píng)估等研究需求。本品僅用于科研,不用于臨床診斷,產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書(shū)。
  • 別名:
    Becn1Beclin-1 ELISA kit; Coiled-coil myosin-like BCL2-interacting protein ELISA kit; Protein GT197) [Cleaved into: Beclin-1-C 35 kDa; Beclin-1-C 37 kDa] ELISA kit
  • 縮寫(xiě):
  • Uniprot No.:
  • 種屬:
    Rattus norvegicus (Rat)
  • 樣本類(lèi)型:
    serum, plasma, tissue homogenates
  • 檢測(cè)范圍:
    0.156 ng/mL-10 ng/mL
  • 靈敏度:
    0.039 ng/mL
  • 反應(yīng)時(shí)間:
    1-5h
  • 樣本體積:
    50-100ul
  • 檢測(cè)波長(zhǎng):
    450 nm
  • 研究領(lǐng)域:
    Immunology
  • 測(cè)定原理:
    quantitative
  • 測(cè)定方法:
    Sandwich
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of rat BECN1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)  
    1:1 Average % 86  
    Range % 83-90  
    1:2 Average % 95  
    Range % 91-99  
    1:4 Average % 91  
    Range % 87-96  
    1:8 Average % 92  
    Range % 87-95  
  • 回收率:
    The recovery of rat BECN1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 96 92-98  
    EDTA plasma (n=4) 90 86-95  
                 
                 
  • 標(biāo)準(zhǔn)曲線:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average Corrected  
    10 2.123 1.982 2.053 1.964  
    5 1.539 1.523 1.531 1.442  
    2.5 1.017 1.001 1.009 0.920  
    1.25 0.691 0.687 0.689 0.600  
    0.625 0.422 0.412 0.417 0.328  
    0.312 0.268 0.259 0.264 0.175  
    0.156 0.166 0.161 0.164 0.075  
    0 0.091 0.087 0.089    
  • 數(shù)據(jù)處理:
  • 貨期:
    3-5 working days

引用文獻(xiàn)

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能:
    Plays a central role in autophagy. Acts as core subunit of the PI3K complex that mediates formation of phosphatidylinositol 3-phosphate; different complex forms are believed to play a role in multiple membrane trafficking pathways: PI3KC3-C1 is involved in initiation of autophagosomes and PI3KC3-C2 in maturation of autophagosomes and endocytosis. Involved in regulation of degradative endocytic trafficking and required for the abcission step in cytokinesis, probably in the context of PI3KC3-C2. Essential for the formation of PI3KC3-C2 but not PI3KC3-C1 PI3K complex forms. Involved in endocytosis. May play a role in antiviral host defense.; Beclin-1-C 35 kDa localized to mitochondria can promote apoptosis; it induces the mitochondrial translocation of BAX and the release of proapoptotic factors.
  • 基因功能參考文獻(xiàn):
    1. Results show decreased SRF levels in the substantia nigra (SN) of rotenone-treated rats that was associated with the loss of tyrosine hydroxylase (TH)-positive neurons. SRF expression was also reduced in rotenone-treated PC12 cells in vitro. In addition, Srf knockdown augmented rotenone-induced toxicity in PC12 cells. In contrast, overexpression of Srf attenuated the cells' sensitivity to rotenone. PMID: 29196028
    2. Expression of Beclin-1 and LAMP-2 in Rat Hippocampus under Conditions of Simulated Gravity Overload in the Caudocranial Vector PMID: 29797121
    3. Data suggested that Beclin-1-mediated autophagy impairs the expression and function of cleaved caspase-8 to protect against cadmium-induced activation of apoptosis through Fas/FasL signaling pathway. PMID: 28428545
    4. that after Caspase 3-mediated Beclin 1 cleavage at 146/149 sites, the N-terminal fragments of Beclin 1 may partially translocate into nuclei in neurons subjected to AD-like injury PMID: 28964771
    5. Lutein-induced autophagy was mediated by the upregulation of BECN1 in IEC-6 cells. PMID: 28893091
    6. treatment with an autophagy inhibitor decreased LC3II expression.However, miR-30b overexpression decreased Pi-induced expression of autophagy-related marker genes such as BECN1, ATG5, and LC3b, whereas miR-30b downregulation increased Pi-induced expression of these genes. In addition, we found that miR-30b PMID: 28578340
    7. These results suggest that EA protects against ischemic stroke through the inhibition of autophagosome formation and autophagy, which is mediated through the mTORC1-ULK complex-Beclin1 pathway PMID: 26647915
    8. in-vitro genetic depletion of beclin-1 in high glucose treated adult rat cardiomyocytes markedly inhibited the level of autophagy and subsequent apoptotic cell death. PMID: 26386349
    9. Hypoxia-affected fetal renal development was associated with renal apoptosis and Beclin 1 signaling-mediated autophagy. PMID: 24872334
    10. Beclin 1 has an essential role as a regulator between autophagy and apoptosis in the setting of neointimal formation PMID: 25238224
    11. veal a novel function of GA binding protein in the regulation of autophagy via transcriptional activation of the BECN1-PIK3C3 complex PMID: 25046113
    12. These results suggest that IPC inhibits Beclin 1-dependent excessive autophagy in reperfusion phase and cooperates with anti-apoptosis pathway to diminish the cell death induced by the myocardial I/R injury. PMID: 23392707
    13. Significantly higher level of Beclin-1 and LC3B-II/LC3B-I ratio (both definitive autophagy biomarkers) was observed in the fibrillated myocardium, versus tissue from the nonfibrillated hearts PMID: 23590156
    14. Rhes robustly binds the autophagy regulator Beclin-1, decreasing its inhibitory interaction with Bcl-2 independent of JNK-1 signaling. PMID: 24324270
    15. Level of beclin-1 in acute on chronic liver failure was lower than control. PMID: 24579466
    16. The results of yhis study found that levels of autophagy markers such as LC3 and Beclin 1 are elevated in the GABAergic interneurons of the spinal dorsal horn following spinal nerve ligation-induced neuropathic pain. PMID: 23665054
    17. Beclin-1 upregulation results in enhanced levels of autophagy. PMID: 22917477
    18. beta-asarone can increase Bcl-2 level, finally interfere with the functions of Beclin 1 during the execution of autophagy. PMID: 22306244
    19. Inhibition of autophagy by Beclin 1 knockdown can attenuate the secondary thalamic damage after focal cerebral infarction. PMID: 22108007
    20. beclin1 may be involved in the pathogenesis of diabetic nephropathy with the mechanism related to the process of programmed cell death. PMID: 21866636
    21. Beclin 1-independent autophagy is an important contributor to both the caspase-dependent and -independent components of neuronal apoptosis and may be considered as an important therapeutic target in neural conditions involving apoptosis PMID: 21646862
    22. Data reveal that Beclin 1 and ATG5 play key roles in morphine-induced autophagy, which may contribute to morphine-induced neuronal injury. PMID: 20190558
    23. Hypoxia triggers a feedback mechanism that delays apoptosis of oxygen-deprived malignant intestinal epithelial cells and is driven by hypoxia-induced Beclin 1 downregulation. PMID: 19901551
    24. The inhibition of autophagy by urocortin is mediated in part by inhibition of Beclin1 expression, an effect which is mediated by activation of the PI3 kinase/Akt pathway but which does not involve activation of p42/p44 MAPK. PMID: 16697404
    25. Beclin 1 protein began to elevate at 1 h time point post-TBI in neurons, 3 d later in astrocytes, and peaked at about 8 d post-TBI. PMID: 18500386
    26. stimulation of autophagy by -methyl D-aspartate 2B glutamate signaling may be regulated by redistribution of Beclin-1 in membrane rafts PMID: 19335206
    27. the AMPK-MEK/ERK-TSC-mTOR pathway regulation of Beclin 1 represents different thresholds responsible for a protective or destructive autophagy PMID: 19520853
    28. Data show that RNA interference-mediated downregulation of Beclin1 improves outcomes after transient middle cerebral artery occlusion. PMID: 19574998

    顯示更多

    收起更多

  • 亞細(xì)胞定位:
    Cytoplasm. Golgi apparatus, trans-Golgi network membrane; Peripheral membrane protein. Endosome membrane; Peripheral membrane protein. Endoplasmic reticulum membrane; Peripheral membrane protein. Mitochondrion membrane; Peripheral membrane protein. Cytoplasmic vesicle, autophagosome.; [Beclin-1-C 35 kDa]: Mitochondrion. Nucleus. Cytoplasm.; [Beclin-1-C 37 kDa]: Mitochondrion.
  • 蛋白家族:
    Beclin family
  • 數(shù)據(jù)庫(kù)鏈接:


主站蜘蛛池模板: 欧美巨大巨粗黑人性aaaaaa| 男女性爽大片视频免费看| 国产三级在线观看播放视频| 精品国产亚洲一区二区三区| 影音先锋在线资源无码| 久久综合久久88中字幕文| 艳妇乳肉豪妇荡乳在线观看| 亚洲一区二区三区四区五区六| 亚洲日韩精品a∨片无码加勒比| 亚洲婷婷综合色香五月| 好爽好湿好硬好大免费视频| 人妻人人做人碰人人添| 黄网站欧美内射| 无码不卡中文字幕av| 久久精品www人人做人人爽| 毛片免费视频观看| 少妇性俱乐部纵欲狂欢电影| 精品国产乱码久久久久久下载| 男女边吃奶边做边爱视频| 久久丫精品久久丫| 国产精品一区二区av| 国语自产精品视频在 视频| 国产精品乱码高清在线观看| 亚洲精品亚洲人成人网| 国产α片免费观看在线人 | 亚洲综合欧美制服丝袜| 久久久噜噜噜www成人网| 欧妇女乱妇女乱视频| 亚洲精品你懂的在线观看| 2021久久最新国产精品| 影音先锋无码aⅴ男人资源站| 人妻无码一区二区三区av| 中文字幕乱码一区二区免费| 欧洲无码一区二区三区在线观看| 亚洲精品一区二区三区蜜臀| 国产ae86亚洲福利入口| 国产精品久久久久无码av1| 欧美乱妇高清无乱码在线观看 | 人妻中文乱码在线网站| 最爽free性欧美人妖| 18处破外女出血在线|