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Rat Alpha-fetoprotein,AFP ELISA Kit

  • 中文名稱:
    大鼠甲種胎兒球蛋白/甲胎蛋白(AFP)酶聯免疫試劑盒
  • 貨號:
    CSB-E08281r
  • 規格:
    96T/48T
  • 價格:
    ¥3900/¥2500
  • 其他:

產品詳情

  • 產品描述:
    大鼠甲種胎兒球蛋白/甲胎蛋白(AFP)酶聯免疫試劑盒(CSB-E08281r)為雙抗夾心法ELISA試劑盒,定量檢測血清、血漿、組織培養上清液、組織勻漿樣本中的AFP含量。AFP即甲胎蛋白,是一種糖蛋白,主要由胎兒肝細胞及卵黃囊合成。在成人,AFP升高可能與肝癌、生殖細胞腫瘤等惡性腫瘤相關。研究機制上,它可作為腫瘤標志物輔助診斷、監測病情。其產生機制可能與腫瘤細胞的異常表達有關。試劑盒檢測范圍為15.6 pg/mL-1000 pg/mL,該產品可廣泛應用于肝癌動物模型研究、干細胞分化監測、組織再生機制探索及腫瘤相關信號通路分析等科研領域。本品僅用于科研,不用于臨床診斷,產品具體參數及操作步驟詳見產品說明書。
  • 別名:
    AfpAlpha-fetoprotein ELISA Kit; Alpha-1-fetoprotein ELISA Kit; Alpha-fetoglobulin ELISA Kit
  • 縮寫:
  • Uniprot No.:
  • 種屬:
    Rattus norvegicus (Rat)
  • 樣本類型:
    serum, plasma, cell culture supernates, tissue homogenates
  • 檢測范圍:
    15.6 pg/mL-1000 pg/mL
  • 靈敏度:
    3.9 pg/mL
  • 反應時間:
    1-5h
  • 樣本體積:
    50-100ul
  • 檢測波長:
    450 nm
  • 研究領域:
    Cancer
  • 測定原理:
    quantitative
  • 測定方法:
    Sandwich
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of rat AFP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
     SampleSerum(n=4)
    1:20Average %85
    Range %81-90
    1:40Average %89
    Range %82-94
    1:80Average %101
    Range %96-108
    1:160Average %94
    Range %89-99
  • 回收率:
    The recovery of rat AFP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9086-94
    EDTA plasma (n=4)9793-102
  • 標準曲線:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    10002.701 2.631 2.666 2.478
    5002.239 2.212 2.226 2.038
    2501.710 1.663 1.687 1.499
    1251.097 1.174 1.136 0.948
    62.50.651 0.695 0.673 0.485
    31.20.427 0.459 0.443 0.255
    15.60.337 0.317 0.327 0.139
    00.192 0.184 0.188  
  • 數據處理:
  • 貨期:
    3-5 working days

引用文獻

產品評價

靶點詳情

  • 功能:
    Binds estrogens, fatty acids and metals.
  • 亞細胞定位:
    [Isoform 1]: Secreted.; [Isoform 2]: Cytoplasm.
  • 蛋白家族:
    ALB/AFP/VDB family
  • 組織特異性:
    Plasma.
  • 數據庫鏈接:


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