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Rat Agrin(AGRN) ELISA kit

  • 中文名稱:
    大鼠聚集蛋白(AGRN)酶聯免疫試劑盒
  • 貨號:
    CSB-EL001461RA
  • 規格:
    96T/48T
  • 價格:
    ¥3600/¥2500
  • 其他:

產品詳情

  • 產品描述:
    大鼠聚集蛋白(AGRN)酶聯免疫試劑盒(CSB-EL001461RA)為雙抗夾心法ELISA試劑盒,定量檢測血清、血漿、組織勻漿樣本中的AGRN含量。AGRN即聚集蛋白,在神經肌肉接頭形成過程中起關鍵作用。它是一種糖蛋白。研究機制方面,它能與肌肉上的受體結合,誘導乙酰膽堿受體聚集,對神經肌肉信號傳遞至關重要,相關研究有助于理解神經肌肉疾病機制。試劑盒檢測范圍為78 pg/mL-5000 pg/mL,靈敏度為19.5 pg/mL。廣泛應用于神經科學、肌肉發育及退行性病變機制研究,特別適用于動物模型中AGRN表達調控分析、藥物干預效果評估及基礎科研中蛋白功能探索,為神經肌肉系統相關疾病的分子機制研究提供可靠工具。本品僅用于科研,不用于臨床診斷,產品具體參數及操作步驟詳見產品說明書。
  • 別名:
    Agrn ELISA kit; AgrinAgrin [Cleaved into: Agrin N-terminal 110 kDa subunit; Agrin C-terminal 110 kDa subunit; Agrin C-terminal 90 kDa fragment ELISA kit; C90); Agrin C-terminal 22 kDa fragment ELISA kit; C22)] ELISA kit
  • 縮寫:
  • Uniprot No.:
  • 種屬:
    Rattus norvegicus (Rat)
  • 樣本類型:
    serum, plasma, tissue homogenates
  • 檢測范圍:
    78 pg/mL-5000 pg/mL
  • 靈敏度:
    19.5 pg/mL
  • 反應時間:
    1-5h
  • 樣本體積:
    50-100ul
  • 檢測波長:
    450 nm
  • 研究領域:
    Neuroscience
  • 測定原理:
    quantitative
  • 測定方法:
    Sandwich
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of rat AGRN in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)  
    1:1 Average % 93  
    Range % 90-96  
    1:2 Average % 84  
    Range % 80-88  
    1:4 Average % 83  
    Range % 80-86  
    1:8 Average % 89  
    Range % 86-91  
  • 回收率:
    The recovery of rat AGRN spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 100 97-103  
    EDTA plasma (n=4) 96 93-99  
                 
                 
  • 標準曲線:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average Corrected  
    5000 2.177 2.198 2.188 2.091  
    2500 1.847 1.863 1.855 1.758  
    1250 1.293 1.273 1.283 1.186  
    625 0.811 0.846 0.829 0.732  
    312.5 0.505 0.496 0.501 0.404  
    156 0.301 0.321 0.311 0.214  
    78 0.188 0.189 0.189 0.092  
    0 0.096 0.098 0.097    
  • 數據處理:
  • 貨期:
    3-5 working days

產品評價

靶點詳情

  • 功能:
    heparan sulfate basal lamina glycoprotein that plays a central role in the formation and the maintenance of the neuromuscular junction (NMJ) and directs key events in postsynaptic differentiation. This neuron-specific (z+) isoform is a component of the AGRN-LRP4 receptor complex that induces the phosphorylation and activation of MUSK. The activation of MUSK in myotubes induces the formation of NMJ by regulating different processes including the transcription of specific genes and the clustering of AChR in the postsynaptic membrane. Calcium ions are required for maximal AChR clustering. AGRN function in neurons is highly regulated by alternative splicing, glycan binding and proteolytic processing. Modulates calcium ion homeostasis in neurons, specifically by inducing an increase in cytoplasmic calcium ions. Functions differentially in the central nervous system (CNS) by inhibiting the alpha(3)-subtype of Na+/K+-ATPase and evoking depolarization at CNS synapses. This transmembrane agrin (TM-agrin) isoform, the predominate form in neurons of the brain, induces dendritic filopodia and synapse formation in mature hippocampal neurons in large part due to the attached glycosaminoglycan chains and the action of Rho-family GTPases.; Isoform 1, isoform 4, isoform 5 and isoform 6: neuron-specific (z+) isoforms that contain C-terminal insertions of 8-19 AA are potent activators of AChR clustering. Isoform 5, agrin (z+8), containing the 8-AA insert, forms a receptor complex in myotubules containing the neuronal AGRN, the muscle-specific kinase MUSK and LRP4, a member of the LDL receptor family. The splicing factors, NOVA1 and NOVA2, regulate AGRN splicing and production of the 'z' isoforms.; is involved in regulation of neurite outgrowth probably due to the presence of the glycosaminoglcan (GAG) side chains of heparan and chondroitin sulfate attached to the Ser/Thr- and Gly/Ser-rich regions. Also involved in modulation of growth factor signaling.; this released fragment is important for agrin signaling and to exert a maximal dendritic filopodia-inducing effect. All 'z' splice variants (z+) of this fragment also show an increase in the number of filopodia.
  • 基因功能參考文獻:
    1. compared expression of agrin isoforms with the expression of AChE-T and ColQ in the developing spinal cord at the stages before and after the formation of NMJ basal lamina PMID: 23117006
    2. present the first crystal structure of an agrin-LRP4 complex which is essential for neuronal agrin-induced acetylcholine receptor (AChR) clustering PMID: 22302937
    3. agrin modulates neuropathic pain through NR1 phosphorylation in GABA neurons PMID: 21068333
    4. a model wherein the z8 insert is a Ca(2+)-responsive allosteric element that is essential in forming an active conformation in neuronal a PMID: 20566625
    5. Agrin binds BMP2, BMP4 and TGFbeta1 with relatively high affinity. PMID: 20505824
    6. different regions within the agrin protein are responsible for synapse formation at the neuromuscular junction and for process formation in central nervous system neurons PMID: 19940118
    7. The expression of agrin was studied during muscle development in a novel three-dimensional muscle-neuronal coculture system. PMID: 12955494
    8. Agrin deletion mutants show that axonal targeting of agrin depends on multiple domains that function in an additive fashion, including the very N-terminal portions and the C-terminal half of the molecule. PMID: 14697677
    9. Agrn becomes concentrated at neuroeffector and neuromuscular juncions in developing urinary bladder. PMID: 15711988
    10. Agrin may play a pivotal role in synaptogenesis in promoting a rapid switch between electrical coupling and synaptic neurotransmission. PMID: 15883200
    11. Agrin plays a role as a regulator of intercellular communication particularly junctional electric coupling and synapatic remodeling. PMID: 16274639
    12. Results suggest that by positively regulating filopodia in developing neurons, transmembrane agrin may influence the pattern of neurite outgrowth and synapse formation. PMID: 16860570
    13. Agrin, released from neurons, controls the maturation of the excitation-contraction coupling mechanism and due to modulation of RyRs and L-type Ca(2+) channels. A role for neural agrin in skeletal muscle maturation is proposed. PMID: 18003748
    14. Agrin plays an important role in the sprouting phase of reactive synaptogenesis; both its expression and distribution are correlated with extent of successful recovery after TBI. PMID: 18627255
    15. increasing the concentration of an ECM molecule, i.e. agrin, may enhance nicotine effects on pCREB PMID: 18639611
    16. transient post-ischemic hypothermia stabilizes brain vessels and reduces blood brain barrier disruption in part by preventing proteolytic degradation of regulatory basement membrane constituents, SPARC and agrin PMID: 19285050
    17. The results suggest a physiological role for Tm-agrin in the maturation of hippocampal neurons involving positive regulation of dendritic filopodia and promotion of synaptogenesis, but also suggest a role for axonal agrin in synaptogenesis. PMID: 19524020

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  • 亞細胞定位:
    Cell junction, synapse. Cell membrane; Single-pass type II membrane protein.
  • 組織特異性:
    Embryonic nervous system and muscle.
  • 數據庫鏈接:

    KEGG: rno:25592

    STRING: 10116.ENSRNOP00000050623

    UniGene: Rn.2163



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