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  4. Rat α2 macroglobulin,α2 MG ELISA Kit

Rat α2 macroglobulin,α2 MG ELISA Kit

  • 中文名稱：
    大鼠α2巨球蛋白(α2-MG)酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-E13863r
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥3800/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    大鼠α2巨球蛋白(α2-MG)酶聯(lián)免疫試劑盒（CSB-E13863r）為競(jìng)爭(zhēng)法ELISA試劑盒，定量檢測(cè)血清、血漿樣本中的A2M含量。A2M（α-2-巨球蛋白）是一種在血漿中含量豐富的糖蛋白，具有蛋白酶抑制作用，參與急性炎癥反應(yīng)。其研究機(jī)制涉及與多種免疫炎癥因子結(jié)合，調(diào)節(jié)體內(nèi)小分子平衡。在神經(jīng)系統(tǒng)疾病中，A2M與阿爾茨海默病、多發(fā)性硬化等疾病相關(guān)，可能通過(guò)空間捕獲蛋白酶來(lái)抑制其活性。試劑盒檢測(cè)范圍為3.13 ng/mL-800 ng/mL，該產(chǎn)品專為科研領(lǐng)域設(shè)計(jì)，可應(yīng)用于炎癥機(jī)制研究、肝腎功能評(píng)估、疾病動(dòng)物模型分析及藥物干預(yù)效果評(píng)價(jià)等方向，為探索α2 - MG在病理生理過(guò)程中的作用提供可靠工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書。
  • 別名：
    A2m ELISA kit; A2m1Alpha-2-macroglobulin ELISA kit; Alpha-2-M ELISA kit
  • 縮寫：
  • Uniprot No.：
  • 種屬：
    Rattus norvegicus (Rat)
  • 樣本類型：
    serum, plasma
  • 檢測(cè)范圍：
    3.13 ng/mL-800 ng/mL
  • 靈敏度：
    3.125 ng/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測(cè)波長(zhǎng)：
    450 nm
  • 研究領(lǐng)域：
    Immunology
  • 測(cè)定原理：
    quantitative
  • 測(cè)定方法：
    Competitive
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat α2 MG in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)
    1:1000 Average % 97
    Range % 88-105
    1:2000 Average % 91
    Range % 89-102
    1:4000 Average % 93
    Range % 90-98
    1:8000 Average % 94
    Range % 87-98
  • 回收率：
    The recovery of rat α2 MG spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range
    Serum (n=5) 97 92-102
    EDTA plasma (n=4) 95 90-99
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average
    800 0.218 0.214 0.216
    200 0.451 0.461 0.456
    50 0.850 0.861 0.856
    12.5 1.225 1.242 1.234
    3.125 1.726 1.737 1.732
    0 1.944 1.967 1.956
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

引用文獻(xiàn)

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    Is able to inhibit all four classes of proteinases by a unique 'trapping' mechanism. This protein has a peptide stretch, called the 'bait region' which contains specific cleavage sites for different proteinases. When a proteinase cleaves the bait region, a conformational change is induced in the protein which traps the proteinase. The entrapped enzyme remains active against low molecular weight substrates (activity against high molecular weight substrates is greatly reduced). Following cleavage in the bait region a thioester bond is hydrolyzed and mediates the covalent binding of the protein to the proteinase.
  • 亞細(xì)胞定位：
    Secreted.
  • 蛋白家族：
    Protease inhibitor I39 (alpha-2-macroglobulin) family
  • 組織特異性：
    Highest constitutive expression in ovary. Low level in testis, uterus and non-acute phase liver. Protein found in plasma.
  • 數(shù)據(jù)庫(kù)鏈接：


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