Rabbit plasminogen activator inhibitor 1,PAI-1 ELISA Kit

產(chǎn)品詳情

說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

兔子纖溶酶原激活物抑制因子1(PAI-1)酶聯(lián)免疫試劑盒（CSB-E06978Rb）為競爭法ELISA試劑盒，定量檢測血清、血漿、組織培養(yǎng)上清液、組織勻漿樣本中的PAI-1含量。PAI - 1即纖溶酶原激活物抑制物 - 1，它在體內(nèi)參與纖溶系統(tǒng)的平衡調(diào)節(jié)。其異常表達(dá)與多種疾病如血栓形成、腫瘤轉(zhuǎn)移等密切相關(guān)。研究機(jī)制主要圍繞調(diào)控其活性及表達(dá)，以改善纖溶功能、影響疾病進(jìn)程，為相關(guān)疾病治療提供思路。試劑盒檢測范圍為6.25 pg/mL-400 pg/mL，適用于心血管疾病模型、器官纖維化機(jī)制研究、藥物干預(yù)效果評(píng)估等科研場景，為探究PAI - 1在病理生理過程中的動(dòng)態(tài)變化提供可靠工具本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
縮寫：

PAI-1
種屬：

Oryctolagus cuniculus (Rabbit)
樣本類型：

serum, plasma, cell culture supernates, tissue homogenates
檢測范圍：

6.25 pg/mL-400 pg/mL
靈敏度：

1.56 pg/mL
反應(yīng)時(shí)間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Cell Biology
測定原理：

quantitative
測定方法：

Competitive

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rabbit PAI-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:20	Average %	87
	Range %	83-90
1:40	Average %	100
	Range %	97-103
1:80	Average %	96
	Range %	90-102
1:160	Average %	90
	Range %	85-95

回收率：

The recovery of rabbit PAI-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	93	80-86
EDTA plasma (n=4)	96	93-99

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.