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  4. Rabbit monocyte chemotactic protein 1/monocyte chemotactic and activating factor,MCP1/MCAF ELISA kit

Rabbit monocyte chemotactic protein 1/monocyte chemotactic and activating factor,MCP1/MCAF ELISA kit

  • 中文名稱：
    兔子單核細胞趨化蛋白1(MCP-1/CCL2/MCAF)酶聯免疫試劑盒
  • 貨號：
    CSB-E06921Rb
  • 規格：
    96T/48T
  • 價格：
    ￥3800/￥2500
  • 其他：

產品詳情

  • 產品描述：
    兔子單核細胞趨化蛋白1(MCP-1/CCL2/MCAF)酶聯免疫試劑盒（CSB-E06921Rb）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的CCL2含量。CCL2是趨化因子家族的重要成員。其背景在于它能趨化單核細胞、記憶T淋巴細胞和樹突狀細胞等至炎癥部位。研究機制方面，它主要通過與CCR2等受體結合，激活下游信號通路，參與炎癥反應、腫瘤進展等多種病理生理過程。試劑盒檢測范圍為23.5 pg/mL-1500 pg/mL，適用于體外研究炎癥性疾病動物模型、免疫細胞趨化實驗或相關信號通路機制探索等科研場景本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
  • 別名：
    CCL2 ELISA Kit; SCYA2C-C motif chemokine 2 ELISA Kit; Monocyte chemoattractant protein 1 ELISA Kit; Monocyte chemotactic protein 1 ELISA Kit; MCP-1 ELISA Kit; Small-inducible cytokine A2 ELISA Kit
  • 縮寫：
  • Uniprot No.：
  • 種屬：
    Oryctolagus cuniculus (Rabbit)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測范圍：
    23.5 pg/mL-1500 pg/mL
  • 靈敏度：
    5.87 pg/mL
  • 反應時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領域：
    Immunology
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rabbit MCP1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1Average %94
    Range %90-100
    1:2Average %95
    Range %91-100
    1:4Average %95
    Range %89-101
    1:8Average %97
    Range %91-100
  • 回收率：
    The recovery of rabbit MCP1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9691-99
    EDTA plasma (n=4)9894-104
  • 標準曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    15002.488 2.501 2.495 2.322
    7501.985 1.999 1.992 1.819
    3751.483 1.471 1.477 1.304
    187.50.938 0.942 0.940 0.767
    940.574 0.584 0.579 0.406
    470.382 0.396 0.389 0.216
    23.50.251 0.253 0.252 0.079
    00.174 0.172 0.173 　
  • 數據處理：
  • 貨期：
    3-5 working days

產品評價

靶點詳情

  • 功能：
    Acts as a ligand for C-C chemokine receptor CCR2. Signals through binding and activation of CCR2 and induces a strong chemotactic response and mobilization of intracellular calcium ions. Exhibits a chemotactic activity for monocytes and basophils but not neutrophils or eosinophils. Plays an important role in mediating peripheral nerve injury-induced neuropathic pain. Increases NMDA-mediated synaptic transmission in both dopamine D1 and D2 receptor-containing neurons, which may be caused by MAPK/ERK-dependent phosphorylation of GRIN2B/NMDAR2B.
  • 基因功能參考文獻：
    1. Data show that peripheral blood endothelial progenitor cells can increase the expressions of chemokines CCL2 and CXCL12 and promote the migration of enhanced green fluorescent protein-positive bone marrow stromal cells to bone defect site. PMID: 26728372
    2. Data show that the serum levels of mast cell tryptase, monocyte chemoattractant protein-1 (MCP-1) and interleukin-8 (IL-8) decreased significantly in Dachengqi decoction treatment group. PMID: 25270202
    3. measurements of CCL2 and macrophages can serve as new inflammatory biomarkers to evaluate the safety of promising novel microbicides for prevention of HIV. PMID: 19929801
    4. Expression of MCP-1 was successfully inhibited by transfecting MCP-1 siRNA expression plasmid to the carotid artery, and the progression of atherosclerosis was restricted by RNAi-mediated silencing of MCP-1 expression. PMID: 19467827
  • 亞細胞定位：
    Secreted.
  • 蛋白家族：
    Intercrine beta (chemokine CC) family
  • 數據庫鏈接：


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