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Rabbit matrix metalloproteinase 3,MMP-3 ELISA kit

  • 中文名稱:
    兔子基質金屬蛋白酶3(MMP-3)酶聯免疫試劑盒
  • 貨號:
    CSB-E09269Rb
  • 規格:
    96T/48T
  • 價格:
    ¥4200/¥3000
  • 其他:

產品詳情

  • 產品描述:
    兔子基質金屬蛋白酶3(MMP-3)酶聯免疫試劑盒(CSB-E09269Rb)為雙抗夾心法ELISA試劑盒,定量檢測血清、血漿、組織勻漿樣本中的MMP3含量。MMP3 即基質金屬蛋白酶 3,它在細胞外基質的降解和重塑等生理過程中發揮重要作用。其異常表達與腫瘤侵襲轉移、關節炎等疾病發展密切相關。研究機制主要聚焦于其對基質成分的作用、信號通路的影響等,為相關疾病治療提供靶點思路。試劑盒檢測范圍為0.156 ng/mL-10 ng/mL,適用于體外培養細胞、動物模型組織等科研場景中MMP-3的動態監測,為研究結締組織代謝調控、炎癥性疾病機制及藥物干預效果提供可靠工具本品僅用于科研,不用于臨床診斷,產品具體參數及操作步驟詳見產品說明書。
  • 別名:
    MMP3 ELISA Kit; Stromelysin-1 ELISA Kit; SL-1 ELISA Kit; EC 3.4.24.17 ELISA Kit; Matrix metalloproteinase-3 ELISA Kit; MMP-3 ELISA Kit; Transin-1 ELISA Kit
  • 縮寫:
  • Uniprot No.:
  • 種屬:
    Oryctolagus cuniculus (Rabbit)
  • 樣本類型:
    serum, plasma, tissue homogenates
  • 檢測范圍:
    0.156 ng/mL-10 ng/mL
  • 靈敏度:
    0.039 ng/mL
  • 反應時間:
    1-5h
  • 樣本體積:
    50-100ul
  • 檢測波長:
    450 nm
  • 研究領域:
    Cancer
  • 測定原理:
    quantitative
  • 測定方法:
    Sandwich
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of rabbit MMP-3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)  
    1:5 Average % 84  
    Range % 80-94  
    1:10 Average % 97  
    Range % 91-105  
    1:20 Average % 93  
    Range % 85-97  
    1:40 Average % 94  
    Range % 86-99  
  • 回收率:
    The recovery of rabbit MMP-3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 96 89-98  
    EDTA plasma (n=4) 89 85-96  
                 
                 
  • 標準曲線:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average Corrected  
    10 2.794 2.694 2.744 2.593  
    5 2.244 2.144 2.194 2.043  
    2.5 1.424 1.324 1.374 1.223  
    1.25 0.854 0.824 0.839 0.688  
    0.625 0.546 0.536 0.541 0.390  
    0.312 0.361 0.351 0.356 0.205  
    0.156 0.230 0.220 0.225 0.074  
    0 0.152 0.149 0.151    
  • 數據處理:
  • 貨期:
    3-5 working days

產品評價

靶點詳情

  • 功能:
    Can degrade fibronectin, laminin, gelatins of type I, III, IV, and V; collagens III, IV, X, and IX, and cartilage proteoglycans. Activates procollagenase.
  • 基因功能參考文獻:
    1. Compromised autophagy may be related to the osteoarthritis progression; JNK and p38 MAPKs up-regulate MMP3 and down-regulate autophagy. PMID: 26873249
    2. the total content and active forms of matrix metalloproteinases (MMPs)-1, -3, and -13 was measured to assess the potential role of the mechanical environment in regulation of matrix turnover by ligament fibroblasts. PMID: 23879595
    3. chitosan-pDNA nanoparticles encoding shRNA targeting MMP-3 and -13 had great potential in silencing the dedifferentiation-related genes for regenerating prolonged and endurable cartilage. PMID: 23520014
    4. Ulinastatin effectively inhibited the increased expression of MMP-2, MMP-3, and iNOS in degenerated NP cells induced by IL-1beta in vitro. PMID: 22853554
    5. Exposure to long periods of light irrespective of its characteristics leads to the increased expression of some matrix metalloprpteases. PMID: 20238014
    6. Tongxinluo can inhibit the expression of MMP-3 and 9 and increase the expression of PPARgamma in atherosclerotic rabbits. PMID: 19778818

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  • 亞細胞定位:
    Secreted, extracellular space, extracellular matrix.
  • 蛋白家族:
    Peptidase M10A family
  • 數據庫鏈接:


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