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Rabbit cholesteryl ester transfer protein,CETP ELISA Kit

  • 中文名稱:
    兔子膽固醇酯轉移蛋白(CETP)酶聯免疫試劑盒
  • 貨號:
    CSB-E06841Rb
  • 規格:
    96T/48T
  • 價格:
    ¥4200/¥3000
  • 其他:

產品詳情

  • 產品描述:
    兔子膽固醇酯轉移蛋白(CETP)酶聯免疫試劑盒(CSB-E06841Rb)為競爭法ELISA試劑盒,定量檢測血清、血漿、組織勻漿樣本中的CETP含量。CETP(膽固醇酯轉運蛋白)是一種血漿糖蛋白,其功能是促進膽固醇酯從高密度脂蛋白(HDL)轉移到低密度脂蛋白(LDL)和極低密度脂蛋白(VLDL),從而影響血脂水平。CETP抑制劑通過抑制CETP活性,增加血清HDL-C水平并降低LDL-C水平,被視為治療血脂異常的潛在方法。試劑盒檢測范圍為1.56 ng/mL-100 ng/mL,科研人員可將其應用于高脂血癥模型兔的血清標志物分析、脂蛋白代謝通路研究或針對CETP抑制劑的藥效學評價,可為脂質代謝機制研究、動物模型評估及藥物開發中CETP活性調控分析提供可靠工具。本品僅用于科研,不用于臨床診斷,產品具體參數及操作步驟詳見產品說明書。
  • 別名:
    CETPCholesteryl ester transfer protein ELISA kit; Lipid transfer protein I ELISA kit; Fragment ELISA kit
  • 縮寫:
  • Uniprot No.:
  • 種屬:
    Oryctolagus cuniculus (Rabbit)
  • 樣本類型:
    serum, plasma, tissue homogenates
  • 檢測范圍:
    1.56 ng/mL-100 ng/mL
  • 靈敏度:
    0.39 ng/mL
  • 反應時間:
    1-5h
  • 樣本體積:
    50-100ul
  • 檢測波長:
    450 nm
  • 研究領域:
    Metabolism
  • 測定原理:
    quantitative
  • 測定方法:
    Competitive
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of rabbit CETP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)  
    1:100 Average % 99  
    Range % 94-102  
    1:200 Average % 97  
    Range % 94-100  
    1:400 Average % 83  
    Range % 80-86  
    1:800 Average % 91  
    Range % 87-95  
  • 回收率:
    The recovery of rabbit CETP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 100 97-103  
    EDTA plasma (n=4) 84 81-89  
                 
                 
  • 標準曲線:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average    
    100 0.082 0.086 0.084    
    50 0.169 0.178 0.174    
    25 0.241 0.258 0.250    
    12.5 0.361 0.378 0.370    
    6.25 0.582 0.541 0.562    
    3.12 0.878 0.898 0.888    
    1.56 1.208 1.231 1.220    
    0 2.015 2.068 2.042    
  • 數據處理:
  • 貨期:
    3-5 working days

產品評價

靶點詳情

  • 功能:
    Involved in the transfer of neutral lipids, including cholesteryl ester and triglyceride, among lipoprotein particles. Allows the net movement of cholesteryl ester from high density lipoproteins/HDL to triglyceride-rich very low density lipoproteins/VLDL, and the equimolar transport of triglyceride from VLDL to HDL. Regulates the reverse cholesterol transport, by which excess cholesterol is removed from peripheral tissues and returned to the liver for elimination.
  • 亞細胞定位:
    Secreted.
  • 蛋白家族:
    BPI/LBP/Plunc superfamily, BPI/LBP family
  • 組織特異性:
    Expressed by the liver.
  • 數據庫鏈接:


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