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  4. Rabbit apolipoprotein A1 (Apo-A1) ELISA Kit

Rabbit apolipoprotein A1 (Apo-A1) ELISA Kit

  • 中文名稱：
    兔載脂蛋白A1(Apo-A1)酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-E09804Rb
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥4200/￥3000
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    兔載脂蛋白A1(Apo-A1)酶聯(lián)免疫試劑盒（CSB-E09804Rb）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿樣本中的APOA1含量。APOA1是重要的血漿載脂蛋白，在膽固醇逆向轉(zhuǎn)運(yùn)中發(fā)揮關(guān)鍵作用。研究發(fā)現(xiàn)，它有助于將外周組織中多余的膽固醇轉(zhuǎn)運(yùn)至肝臟進(jìn)行代謝，降低動(dòng)脈粥樣硬化風(fēng)險(xiǎn)。目前圍繞APOA1開(kāi)展了眾多研究，試圖通過(guò)藥物等手段提升其功能及表達(dá)水平。試劑盒檢測(cè)范圍為6.25 μg/mL-400 μg/mL，適用于心血管疾病機(jī)制研究、藥物干預(yù)效果評(píng)估或營(yíng)養(yǎng)代謝相關(guān)動(dòng)物模型中的生物標(biāo)志物分析。研究人員可通過(guò)該工具動(dòng)態(tài)監(jiān)測(cè)實(shí)驗(yàn)動(dòng)物在疾病發(fā)展或治療過(guò)程中Apo - A1的表達(dá)變化，為探索脂蛋白代謝調(diào)控網(wǎng)絡(luò)及開(kāi)發(fā)相關(guān)干預(yù)策略提供數(shù)據(jù)支持。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書。
  • 別名：
    APOA1Apolipoprotein A-I ELISA kit; Apo-AI ELISA kit; ApoA-I ELISA kit; Apolipoprotein A1) [Cleaved into: Proapolipoprotein A-I ELISA kit; ProapoA-I); Truncated apolipoprotein A-I] ELISA kit
  • 縮寫：
  • Uniprot No.：
  • 種屬：
    Oryctolagus cuniculus (Rabbit)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測(cè)范圍：
    6.25 μg/mL-400 μg/mL
  • 靈敏度：
    1.56 μg/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測(cè)波長(zhǎng)：
    450 nm
  • 研究領(lǐng)域：
    Cardiovascular
  • 測(cè)定原理：
    quantitative
  • 測(cè)定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rabbit Apo-A1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1Average %84
    Range %80-92
    1:2Average %99
    Range %91-105
    1:4Average %89
    Range %80-96
    1:8Average %94
    Range %85-99
  • 回收率：
    The recovery of rabbit Apo-A1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9389-98
    EDTA plasma (n=4)9889-107
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    µg/mlOD1OD2AverageCorrected
    4002.748 2.712 2.730 2.645
    2002.234 2.222 2.228 2.143
    1001.517 1.446 1.482 1.397
    500.875 0.839 0.857 0.772
    250.433 0.424 0.429 0.344
    12.50.212 0.206 0.209 0.124
    6.250.141 0.138 0.140 0.055
    00.087 0.083 0.085 　
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

引用文獻(xiàn)

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    Participates in the reverse transport of cholesterol from tissues to the liver for excretion by promoting cholesterol efflux from tissues and by acting as a cofactor for the lecithin cholesterol acyltransferase (LCAT). As part of the SPAP complex, activates spermatozoa motility.
  • 基因功能參考文獻(xiàn)：
    1. Local HDAdApoAI vascular gene therapy may promote rapid regression of small-to-moderate-sized atherosclerotic lesions. PMID: 27932352
    2. These differentially expressed proteins associated with key mechanisms involved in atherosclerosis and signaling mechanisms related with vitamin E. PMID: 23796494
    3. This study showed that apoA-I exerted protective effects against fatty liver disease in rabbits induced by a high-fat diet, possibly through its antioxidant actions. PMID: 23174676
    4. The molar ratio ApoE/ApoA-I is negatively correlated with the enzyme activity, and positively correlated with increases in the intima-media thickness of common carotid wall and cardiac dysfunction signs. PMID: 22562159
  • 亞細(xì)胞定位：
    Secreted.
  • 蛋白家族：
    Apolipoprotein A1/A4/E family
  • 組織特異性：
    Major protein of plasma HDL, also found in chylomicrons.
  • 數(shù)據(jù)庫(kù)鏈接：


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