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  4. Rabbit Vascular Endothelial cell Growth Factor,VEGF ELISA Kit

Rabbit Vascular Endothelial cell Growth Factor,VEGF ELISA Kit

  • 中文名稱(chēng)：
    兔血管內(nèi)皮細(xì)胞生長(zhǎng)因子(VEGF)酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-E08524Rb
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥4200/￥3000
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    兔血管內(nèi)皮細(xì)胞生長(zhǎng)因子(VEGF)酶聯(lián)免疫試劑盒（CSB-E08524Rb）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿樣本中的VEGF含量。VEGF即血管內(nèi)皮生長(zhǎng)因子，在腫瘤血管生成、傷口愈合等生理過(guò)程中起關(guān)鍵作用。其背景在于它能促進(jìn)內(nèi)皮細(xì)胞增殖、遷移和血管生成。研究機(jī)制方面，主要是與受體結(jié)合，激活下游信號(hào)通路，誘導(dǎo)血管生成，是腫瘤治療等領(lǐng)域的重要靶點(diǎn)。試劑盒檢測(cè)范圍為1.56 pg/mL-100 pg/mL，適用于科研場(chǎng)景下探究血管生成相關(guān)機(jī)制、評(píng)估疾病模型中VEGF的動(dòng)態(tài)變化，或用于藥物研發(fā)中靶向分子效應(yīng)的體外檢測(cè)。可為心血管疾病、腫瘤微環(huán)境等領(lǐng)域的基礎(chǔ)研究提供可靠數(shù)據(jù)支持。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書(shū)。
  • 縮寫(xiě)：
    VEGF
  • 種屬：
    Oryctolagus cuniculus (Rabbit)
  • 樣本類(lèi)型：
    serum, plasma, tissue homogenates
  • 檢測(cè)范圍：
    1.56 pg/mL-100 pg/mL
  • 靈敏度：
    0.39 pg/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測(cè)波長(zhǎng)：
    450 nm
  • 研究領(lǐng)域：
    Signal Transduction
  • 測(cè)定原理：
    quantitative
  • 測(cè)定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rabbit VEGF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1Average %85
    Range %81-95
    1:2Average %100
    Range %91-106
    1:4Average %94
    Range %92-105
    1:8Average %90
    Range %86-98
  • 回收率：
    The recovery of rabbit VEGF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9490-98
    EDTA plasma (n=4)9791-100
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    1002.538 2.540 2.539 2.403
    502.012 2.022 2.017 1.881
    251.137 1.130 1.134 0.998
    12.50.602 0.651 0.627 0.491
    6.250.375 0.378 0.377 0.241
    3.120.279 0.285 0.282 0.146
    1.560.168 0.176 0.172 0.036
    00.131 0.141 0.136 　
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

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相關(guān)問(wèn)答

■ 常見(jiàn)問(wèn)題解答
Q:

Please provide the following information regarding Rabbit Vascular Endothelial cell Growth Factor, VEGF ELISA Kit (CSB-E08524Rb)
a. Can this kit detect human VEGF?

A:
Thanks for your inquiry.
About Vascular Endothelial cell Growth Factor，there is VEGFA～D for human while there is only VEGF for rabbit.
CSB-E08524Rb This kit is designed according to: https://www.uniprot.org/uniprot/Q866G4.
It detects VEGF and it can not test human samples.
Pls let me know if you have any further questions. Thank you.


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