Rabbit Tartrate-resistant acid phosphatase 5b (TRAP5b) ELISA Kit
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中文名稱：兔子抗酒石酸酸性磷酸酶5b(TRAP5b)酶聯(lián)免疫試劑盒
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貨號(hào)：CSB-E15043Rb
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規(guī)格：96T/48T
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價(jià)格：￥4200/￥3000
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其他：
產(chǎn)品詳情
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產(chǎn)品描述：兔子抗酒石酸酸性磷酸酶5b(TRAP5b)酶聯(lián)免疫試劑盒（CSB-E15043Rb）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿樣本中的TRAP5b含量。試劑盒檢測(cè)范圍為78 μU/mL-5000 μU/mL，適用于科研領(lǐng)域中對(duì)骨代謝相關(guān)疾病（如骨質(zhì)疏松、骨轉(zhuǎn)移癌、風(fēng)濕性關(guān)節(jié)炎等）的機(jī)制研究，也可用于藥物開(kāi)發(fā)中破骨細(xì)胞活性調(diào)節(jié)劑的體外效果評(píng)估。通過(guò)檢測(cè)樣本中TRAP5b的表達(dá)水平，研究者可量化分析骨吸收活性變化，為骨骼系統(tǒng)疾病模型構(gòu)建、病理機(jī)制探索及治療策略優(yōu)化提供實(shí)驗(yàn)依據(jù)。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書(shū)。
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別名：ACP5 ELISA Kit; Tartrate-resistant acid phosphatase type 5 ELISA Kit; TR-AP ELISA Kit; EC 3.1.3.2 ELISA Kit; Tartrate-resistant acid ATPase ELISA Kit; TrATPase ELISA Kit; Type 5 acid phosphatase ELISA Kit
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縮寫(xiě)：TRAP5b
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Uniprot No.：
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種屬：Oryctolagus cuniculus (Rabbit)
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樣本類型：serum, plasma, tissue homogenates
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檢測(cè)范圍：78 μU/mL-5000 μU/mL
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靈敏度：19.5 μU/mL
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反應(yīng)時(shí)間：1-5h
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樣本體積：50-100ul
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檢測(cè)波長(zhǎng)：450 nm
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研究領(lǐng)域：Others
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測(cè)定原理：quantitative
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測(cè)定方法：Sandwich
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精密度：
Intra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess. -
線性度：
To assess the linearity of the assay, samples were spiked with high concentrations of rabbit TRAP5b in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. Sample Serum(n=4) 1:1 Average % 97 Range % 88-101 1:2 Average % 88 Range % 81-94 1:4 Average % 97 Range % 87-101 1:8 Average % 91 Range % 84-95 -
回收率：
The recovery of rabbit TRAP5b spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 90 84-96 EDTA plasma (n=4) 103 93-107 -
標(biāo)準(zhǔn)曲線：
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. 
μU/ml OD1 OD2 Average Corrected 5000 1.994 1.912 1.953 1.835 2500 1.776 1.745 1.761 1.643 1250 1.504 1.561 1.533 1.415 625 1.212 1.178 1.195 1.077 312 0.929 0.900 0.915 0.797 156 0.506 0.498 0.502 0.384 78 0.289 0.282 0.286 0.168 0 0.116 0.120 0.118 -
數(shù)據(jù)處理：
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貨期：3-5 working days
引用文獻(xiàn)
- Application of hydroxyapatite nanoparticles in tumor-associated bone segmental defect Kun Zhang, et al,Science Advances,2019
- Tetrandrine enhances the ubiquitination and degradation of Syk through an AhR-csrc-c-Cbl pathway and consequently inhibits osteoclastogenesis and bone destruction in arthritis Jia Y, et al,Cell Death & Disease,2019
- Tetrandrine attenuates the bone erosion in collagen-induced arthritis rats by inhibiting osteoclastogenesis via spleen tyrosine kinase Yugai Jia.et al,The FASEB Journal,2018
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