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Rabbit Sex hormone-binding globulin(SHBG) ELISA kit

  • 中文名稱:
    兔子性激素結合球蛋白(SHBG)酶聯免疫試劑盒
  • 貨號:
    CSB-EL021252RB
  • 規格:
    96T/48T
  • 價格:
    ¥4200/¥3000
  • 其他:

產品詳情

  • 產品描述:
    兔子性激素結合球蛋白(SHBG)酶聯免疫試劑盒(CSB-EL021252RB)為競爭法ELISA試劑盒,定量檢測血清、血漿、組織勻漿樣本中的SHBG含量。SHBG即性激素結合球蛋白,其可與雄激素、雌激素等結合并調節其活性。在它影響體內性激素的分布及代謝。研究機制上,涉及探討其基因表達調控、與性激素結合的分子機制,以及在生殖、代謝等疾病中的作用機制。試劑盒檢測范圍為25 pmol/mL-400 pmol/mL,適用于評估實驗動物性激素代謝狀態、研究生殖系統疾病模型或藥物干預對激素運輸通路的影響,為生殖生理機制、內分泌調控及相關代謝疾病的基礎研究提供高效分析工具。本品僅用于科研,不用于臨床診斷,產品具體參數及操作步驟詳見產品說明書。
  • 別名:
    SHBG ELISA Kit; Sex hormone-binding globulin ELISA Kit; SHBG ELISA Kit; Sex steroid-binding protein ELISA Kit; SBP ELISA Kit; Testis-specific androgen-binding protein ELISA Kit; ABP ELISA Kit
  • 縮寫:
    SHBG
  • Uniprot No.:
  • 種屬:
    Oryctolagus cuniculus (Rabbit)
  • 樣本類型:
    serum, plasma, tissue homogenates
  • 檢測范圍:
    25 pmol/mL-400 pmol/mL
  • 靈敏度:
    25 pmol/mL
  • 反應時間:
    1-5h
  • 樣本體積:
    50-100ul
  • 檢測波長:
    450 nm
  • 研究領域:
    Signal Transduction
  • 測定原理:
    quantitative
  • 測定方法:
    Competitive
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<15%        
    Three samples of known concentration were tested twenty times on one plate to assess.    
    Inter-assay Precision (Precision between assays): CV%<15%        
    Three samples of known concentration were tested in twenty assays to assess.      
                   
  • 線性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of rabbit SHBG in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.  
      Sample Serum(n=4)    
    1:1 Average % 92    
    Range % 90-103    
    1:2 Average % 89    
    Range % 83-99    
    1:4 Average % 95    
    Range % 90-101    
    1:8 Average % 89    
    Range % 85-100    
  • 回收率:
    The recovery of rabbit SHBG spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.  
     
    Sample Type Average % Recovery Range    
    Serum (n=5) 95 87-100    
    EDTA plasma (n=4) 95 89-101    
                   
                   
  • 標準曲線:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.  
     
    pmol/ml OD1 OD2 Average      
    400 0.266 0.252 0.259      
    200 0.528 0.541 0.535      
    100 0.874 0.863 0.869      
    50 1.348 1.325 1.337      
    25 2.511 2.552 2.532      
  • 數據處理:
  • 貨期:
    3-5 working days

產品評價

靶點詳情

  • 功能:
    Functions as an androgen transport protein, but may also be involved in receptor mediated processes. Each dimer binds one molecule of steroid. Specific for 5-alpha-dihydrotestosterone, testosterone, and 17-beta-estradiol. Regulates the plasma metabolic clearance rate of steroid hormones by controlling their plasma concentration.
  • 亞細胞定位:
    Secreted.
  • 數據庫鏈接:


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