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中文名稱:兔子髓過氧化物酶(MPO)酶聯免疫試劑盒
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貨號:CSB-E13689Rb
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規格:96T/48T
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價格:¥4200/¥3000
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其他:
產品詳情
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產品描述:兔子髓過氧化物酶(MPO)酶聯免疫試劑盒(CSB-E13689Rb)為競爭法ELISA試劑盒,定量檢測血清、血漿、組織培養上清液、組織勻漿樣本中的MPO含量。MPO即髓過氧化物酶,是中性粒細胞和單核細胞產生的一種血紅素蛋白。其可催化過氧化氫和氯離子生成次氯酸,在固有免疫中發揮重要作用。異常表達的MPO會產生大量活性氧,與多種炎癥相關疾病和心血管疾病的發病機制有關。試劑盒檢測范圍為1.25 ng/mL-20 ng/mL,適用于基礎科研中炎癥機制研究、免疫相關疾病動物模型評估、藥物干預效果分析等場景,例如通過檢測實驗動物血清或組織樣本中的MPO水平,定量評估中性粒細胞浸潤程度及相關疾病的病理進程,為心血管疾病、神經退行性疾病等領域的機制探索提供可靠數據支持。本品僅用于科研,不用于臨床診斷,產品具體參數及操作步驟詳見產品說明書。
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縮寫:
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Uniprot No.:
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種屬:Oryctolagus cuniculus (Rabbit)
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樣本類型:serum, plasma, cell culture supernates, tissue homogenates
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檢測范圍:1.25 ng/mL-20 ng/mL
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靈敏度:1.25 ng/mL
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反應時間:1-5h
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樣本體積:50-100ul
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檢測波長:450 nm
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研究領域:Immunology
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測定原理:quantitative
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測定方法:Competitive
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精密度:
Intra-assay Precision (Precision within an assay): CV%<15% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<15% Three samples of known concentration were tested in twenty assays to assess. -
線性度:
To assess the linearity of the assay, samples were spiked with high concentrations of rabbit MPO in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. Sample Serum(n=4) 1:1 Average % 96 Range % 91-103 1:2 Average % 87 Range % 84-90 1:4 Average % 95 Range % 89-99 1:8 Average % 101 Range % 98-105 -
回收率:
The recovery of rabbit MPO spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 89 85-94 EDTA plasma (n=4) 93 89-97 -
標準曲線:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. ng/ml OD1 OD2 Average 20 0.204 0.201 0.203 10 0.376 0.398 0.387 5 0.635 0.602 0.619 2.5 1.134 1.122 1.128 1.25 2.339 2.367 2.353 -
數據處理:
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貨期:3-5 working days
引用文獻
- Cinnamaldehyde has ameliorative effects on rabbit spinal cord ischemia and reperfusion injury PK Bekta?o?lu,World neurosurgery,2023
- Cerebrolysin Amelioration of Spinal Cord Ischemia/Reperfusion Injury in Rabbit Model C TONGE,studies,2023
- Systemic injury caused by taurocholate?induced severe acute pancreatitis in rats XX Hong,Clinics,2022
- Phosphatidylethanolamine-Binding Protein 1 Ameliorates Ischemia-Induced Inflammation and Neuronal Damage in the Rabbit Spinal Cord Kim W, et al,Cells,2019
- Tat-protein disulfide-isomerase A3: a possible candidate for preventing ischemic damage in the spinal cord Yoo DY.et al,Cell Death Dis,2017
- Anti-inflammatory cytokine TSG-6 inhibits hypertrophic scar formation in a rabbit ear model Hui Wang et al,European Journal of Pharmacology,2015
- The protective effect of low-dose methotrexate on ischemia-reperfusion injury of the rabbit spinal cord Kertmen H et al,Eur J Pharmacol,2013
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