在线日韩日本国产亚洲丨少妇伦子伦情品无吗丨欧美性猛交xxxx免费看蜜桃丨精品人妻系列无码一区二区三区丨亚洲精品无码不卡在线播放

Your Good Partner in Biology Research

  1. 首頁(yè)
  2. 試劑盒
  3. 酶聯(lián)免疫試劑盒
  4. Rabbit Interleukin 8,IL-8 ELISA Kit
  • 中文名稱：
    兔子白介素8(IL-8/CXCL8)酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-E06905Rb
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥3800/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    兔子白介素8(IL-8/CXCL8)酶聯(lián)免疫試劑盒（CSB-E06905Rb）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿樣本中的CXCL8含量。CXCL8是一種趨化因子，在炎癥反應(yīng)、免疫調(diào)節(jié)等生理病理過(guò)程中發(fā)揮重要作用。其研究機(jī)制主要聚焦于與受體結(jié)合，介導(dǎo)細(xì)胞趨化和激活，可調(diào)節(jié)中性粒細(xì)胞遷移和活化，在感染、腫瘤、自身免疫病等疾病中表達(dá)常異常，是潛在治療靶點(diǎn)。試劑盒檢測(cè)范圍為23.5 pg/mL-1500 pg/mL，適用于實(shí)驗(yàn)室中炎癥機(jī)制研究、免疫調(diào)控實(shí)驗(yàn)及藥物開發(fā)過(guò)程中的生物標(biāo)志物動(dòng)態(tài)監(jiān)測(cè)，例如在細(xì)菌感染模型、創(chuàng)傷修復(fù)研究或抗炎藥物藥效評(píng)估中量化IL - 8的表達(dá)變化本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書。
  • 別名：
    CXCL8 ELISA Kit; IL8Interleukin-8 ELISA Kit; IL-8 ELISA Kit; C-X-C motif chemokine 8 ELISA Kit; Chemokine ELISA Kit; C-X-C motif) ligand 8 ELISA Kit; Neutrophil attractant/activation protein 1 ELISA Kit; NAP-1 ELISA Kit; Permeability factor 1 ELISA Kit; PF1 ELISA Kit; rPF1 ELISA Kit
  • 縮寫：
  • Uniprot No.：
  • 種屬：
    Oryctolagus cuniculus (Rabbit)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測(cè)范圍：
    23.5 pg/mL-1500 pg/mL
  • 靈敏度：
    5.8 pg/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測(cè)波長(zhǎng)：
    450 nm
  • 研究領(lǐng)域：
    Immunology
  • 測(cè)定原理：
    quantitative
  • 測(cè)定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rabbit IL-8 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1Average %101
    Range %94-105
    1:2Average %97
    Range %91-102
    1:4Average %89
    Range %85-94
    1:8Average %90
    Range %86-98
  • 回收率：
    The recovery of rabbit IL-8 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9387-96
    EDTA plasma (n=4)9289-94
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    15002.390 2.345 2.368 2.285
    7501.587 1.476 1.532 1.449
    3750.814 0.778 0.796 0.713
    187.50.409 0.389 0.399 0.316
    940.276 0.264 0.270 0.187
    470.182 0.172 0.177 0.094
    23.50.134 0.131 0.133 0.050
    00.085 0.081 0.083 　
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評(píng)價(jià)

平均分:
5.0分 - 1 個(gè)評(píng)價(jià)

樣品類型：血漿（抗凝劑） EDTA

樣品信息：其他 兔

稀釋比：沒(méi)有稀釋

產(chǎn)品評(píng)價(jià)： 我用CSB-E06905Rb檢測(cè)模型組術(shù)前，術(shù)中30min-2h，術(shù)后24h血漿，標(biāo)準(zhǔn)曲線用四參數(shù)模型擬合出來(lái)的R2為0.9998，見(jiàn)圖片，OD值范圍為0.04（Blank)-2.5(1500pg/ml)，標(biāo)準(zhǔn)曲線符合預(yù)期。部分樣本低于標(biāo)曲的Blank吸光度，經(jīng)過(guò)文獻(xiàn)查詢，該模型需要6h才能檢測(cè)出大量IL-8炎癥因子水平，因此屬于正常現(xiàn)象。且得到的數(shù)據(jù)符合預(yù)期趨勢(shì)，后續(xù)會(huì)測(cè)量6h及以上的炎癥因子水平。另回答郵件中的疑問(wèn)：我做的是術(shù)前，術(shù)中0-2h，術(shù)后24h共6個(gè)點(diǎn)的指標(biāo)，沒(méi)做6h的指標(biāo)，因?yàn)?h兔子耳朵采不出動(dòng)脈血了，所以后續(xù)會(huì)在6h處死兔子來(lái)檢測(cè)炎癥指標(biāo)。

By 陳老師

靶點(diǎn)詳情

  • 功能：
    IL-8 is a chemotactic factor that attracts neutrophils, basophils, and T-cells, but not monocytes. It is also involved in neutrophil activation. It is released from several cell types in response to an inflammatory stimulus.
  • 基因功能參考文獻(xiàn)：
    1. At the 2-wk time point, interleukin 8 gene expression was comparable in intervertebral discs injected with chondrocytes in controls, whereas its expression in IVDs injected with saline increased 50-fold PMID: 25133623
    2. Data show that the serum levels of mast cell tryptase, monocyte chemoattractant protein-1 (MCP-1) and interleukin-8 (IL-8) decreased significantly in Dachengqi decoction treatment group. PMID: 25270202
    3. The JNK pathway plays an important role in mechanical ventilation-stimulated TNF-alpha expression in alveolar macrophages, but the injury-stimulated IL-8 expression may be regulated by other signaling pathways. PMID: 24070709
    4. IL-8 and IL-1ra showed correlation with the severity of colitis. These observations should significantly further understandings on the role of neutrophils and monocytes in the immunopathogenesis of ulcerative colitis PMID: 21807533
  • 亞細(xì)胞定位：
    Secreted.
  • 蛋白家族：
    Intercrine alpha (chemokine CxC) family
  • 數(shù)據(jù)庫(kù)鏈接：


主站蜘蛛池模板： 国产免费福利在线视频| 日本牲交大片免费观看| av无码免费岛国动作片片段欣赏网| 日本无翼乌邪恶大全彩h | 国产一区二区三区在线蜜桃| 亚洲性色成人av| 色久悠悠婷婷综合在线亚洲| 国产精品三级在线观看无码| 成人精品一区日本无码网站 | 国产乱人伦av在线a最新| 精品蜜臀久久久久99网站| 中文字幕日韩精品一区二区三区| 国产高清午夜人成在线观看| 鲁一鲁一鲁一鲁一曰综合网| www夜片内射视频在观看视频| 色综合久久无码中文字幕| 2020国产欧洲精品网站| 久久免费精品国产72精品九九| 99久久亚洲精品日本无码| 国精产品一区一区三区有限公司杨| 综合久久国产九一剧情麻豆| 国内丰满熟女出轨videos| 人与性动交aaaabbbb| 免费特级毛片| 2018亚洲а∨天堂| 午夜福利免费0948视频| 国产婷婷色综合av蜜臀av| 少妇无码av无码专区线| 亚洲综合无码av一区二区三区 | 国产精品成人一区二区三区| 国产精品久久久久久一区二区三区| 国产精品毛片完整版视频| 亚洲高清国产拍精品26u| 农村欧美丰满熟妇xxxx| 国产精品日日做人人爱| 国产精品午夜爆乳美女视频| 亚洲中文字幕无码乱线| 人妻少妇中文字幕乱码| 久久高清内射无套| 亚洲成av人片天堂网久久| 亚洲欧美日韩综合久久久久久|