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Rabbit Interleukin 2,IL-2 ELISA Kit

  • 中文名稱:
    兔子白介素2(IL-2)酶聯免疫試劑盒
  • 貨號:
    CSB-E08745Rb
  • 規格:
    96T/48T
  • 價格:
    ¥3800/¥2500
  • 其他:

產品詳情

  • 產品描述:
    兔子白介素2(IL-2)酶聯免疫試劑盒(CSB-E08745Rb)為雙抗夾心法ELISA試劑盒,定量檢測血清、血漿樣本中的IL2含量。IL2是重要的細胞因子,在免疫調節中發揮關鍵作用。其背景上,是免疫系統抵御疾病的重要介質。研究機制方面,IL2可激活T細胞和NK細胞等免疫細胞,增強其殺傷活性,在腫瘤免疫治療、自身免疫病等研究領域被廣泛關注。試劑盒檢測范圍為31.25 pg/mL-2000 pg/mL,適用于動物模型研究、免疫機制探索、炎癥相關疾病機制分析或疫苗效力評估等科研場景,為探究IL - 2在免疫系統中的調控作用提供可靠工具。本品僅用于科研,不用于臨床診斷,產品具體參數及操作步驟詳見產品說明書。
  • 別名:
    IL2Interleukin-2 ELISA Kit; IL-2 ELISA Kit; T-cell growth factor ELISA Kit; TCGF ELISA Kit
  • 縮寫:
  • Uniprot No.:
  • 種屬:
    Oryctolagus cuniculus (Rabbit)
  • 樣本類型:
    serum, plasma
  • 檢測范圍:
    31.25 pg/mL-2000 pg/mL
  • 靈敏度:
    7.8 pg/mL
  • 反應時間:
    1-5h
  • 樣本體積:
    50-100ul
  • 檢測波長:
    450 nm
  • 研究領域:
    Immunology
  • 測定原理:
    quantitative
  • 測定方法:
    Sandwich
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of rabbit IL-2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
     SampleSerum(n=4)
    1:1Average %87
    Range %82-93
    1:2Average %95
    Range %91-100
    1:4Average %86
    Range %80-95
    1:8Average %93
    Range %82-98
  • 回收率:
    The recovery of rabbit IL-2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9280-98
    EDTA plasma (n=4)9384-100
  • 標準曲線:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    20002.412 2.320 2.366 2.181
    10002.174 2.081 2.128 1.943
    5001.882 1.743 1.813 1.628
    2501.521 1.482 1.502 1.317
    1251.048 1.044 1.046 0.861
    62.50.688 0.654 0.671 0.486
    31.250.331 0.308 0.320 0.135
    00.191 0.179 0.185  
  • 數據處理:
  • 貨期:
    3-5 working days

產品評價

靶點詳情

  • 功能:
    Produced by T-cells in response to antigenic or mitogenic stimulation, this protein is required for T-cell proliferation and other activities crucial to regulation of the immune response. Can stimulate B-cells, monocytes, lymphokine-activated killer cells, natural killer cells, and glioma cells.
  • 基因功能參考文獻:
    1. IL-2 activates the STAT3 pathway, protects the lens epithelium cells and reduce the damage caused by inflammation reactions. PMID: 18201527
  • 亞細胞定位:
    Secreted.
  • 蛋白家族:
    IL-2 family
  • 數據庫鏈接:


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