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  4. Rabbit Interleukin 1β,IL-1β ELISA Kit

Rabbit Interleukin 1β,IL-1β ELISA Kit

  • 中文名稱：
    兔子白介素1β (IL-1β)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-E06900Rb
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥3800/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    兔子白介素1β (IL-1β)酶聯(lián)免疫試劑盒（CSB-E06900Rb）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的IL1B含量。IL1B即白介素-1β，是一種炎癥細胞因子，在炎癥反應、免疫調(diào)節(jié)等生理病理過程中扮演重要角色。研究表明，它能激活下游信號通路，介導炎性反應。異常表達與多種疾病如關(guān)節(jié)炎、心血管疾病等密切相關(guān)，是藥物研發(fā)的熱門靶點。試劑盒檢測范圍為7.8 pg/mL-500 pg/mL，適用于體外實驗中對動物模型（如關(guān)節(jié)炎、膿毒癥等疾病模型）的炎癥機制研究、藥物干預效果評估或免疫調(diào)節(jié)通路分析；為科研工作者在基礎(chǔ)醫(yī)學、藥理學及免疫學領(lǐng)域提供靈敏穩(wěn)定的檢測工具，支持細胞因子網(wǎng)絡(luò)調(diào)控的深入探索。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    IL1BInterleukin-1 beta ELISA kit; IL-1 beta ELISA kit; Lymphocyte proliferation-potentiating factor ELISA kit
  • 縮寫：
  • Uniprot No.：
  • 種屬：
    Oryctolagus cuniculus (Rabbit)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測范圍：
    7.8 pg/mL-500 pg/mL
  • 靈敏度：
    1.95 pg/mL
  • 反應時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Immunology
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rabbit IL-1β in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1Average %98
    Range %85-105
    1:2Average %95
    Range %85-103
    1:4Average %94
    Range %82-100
    1:8Average %95
    Range %85-105
  • 回收率：
    The recovery of rabbit IL-1β spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9682-104
    EDTA plasma (n=4)9585-105
  • 標準曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    5002.852 2.713 2.783 2.674
    2502.235 2.245 2.240 2.131
    1251.424 1.392 1.408 1.299
    62.50.753 0.784 0.769 0.660
    31.20.447 0.433 0.440 0.331
    15.60.266 0.282 0.274 0.165
    7.80.202 0.195 0.199 0.090
    00.111 0.106 0.109 　
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

引用文獻

產(chǎn)品評價

靶點詳情

  • 功能：
    Potent proinflammatory cytokine. Initially discovered as the major endogenous pyrogen, induces prostaglandin synthesis, neutrophil influx and activation, T-cell activation and cytokine production, B-cell activation and antibody production, and fibroblast proliferation and collagen production. Promotes Th17 differentiation of T-cells. Synergizes with IL12/interleukin-12 to induce IFNG synthesis from T-helper 1 (Th1) cells. Plays a role in angiogenesis by inducing VEGF production synergistically with TNF and IL6.
  • 基因功能參考文獻：
    1. Mild heat shock increased the production of inflammatory cytokines, IL-1beta and IL-6 in rabbit cornea cells. PMID: 23330884
    2. IL-1beta and TNF-alpha expression increases significantly during acute lung injury. Ambroxol combined with low-dose heparin inhibits teh release of IL-1beta and TNF-alpha. PMID: 21845882
    3. IL-1beta induced a significant reduction in the relative intrinsic activity of GLUT5 and in this decrease are involved NO signal pathways; blockage of D-fructose intestinal uptake by IL-1beta may play an essential role in the pathophysiology of septic shock. PMID: 20803526
    4. The inhibitory effect of IL-1beta on D-galactose absorption across mucosal side of enterocyte could be mediated by the activation of several kinases and nuclear factor kappa B. PMID: 22759965
    5. Glucosamine hydrochloride treatment can can partially decrease the expression levels of IL-1 beta and increase the expression levels of TGF-beta 1, which delays the development of osteoarthritis. PMID: 20369526
    6. In this study evidence is provided that exogenous PGF2alpha differentially modulates luteal expression of IL1B transcripts depending on luteal stage. PMID: 20427144
    7. results revealed that a transient episode of raised-intensity phonation causes a significant increase in vocal fold inflammatory mRNA expression - IL-1beta,COX-2, and TGFbeta1 PMID: 20879195

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  • 亞細胞定位：
    Cytoplasm, cytosol. Lysosome. Secreted, extracellular exosome. Secreted.
  • 蛋白家族：
    IL-1 family
  • 數(shù)據(jù)庫鏈接：


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