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  4. Rabbit Interferon γ,IFN-γ ELISA Kit

Rabbit Interferon γ,IFN-γ ELISA Kit

  • 中文名稱：
    兔子γ干擾素(IFN-γ)酶聯免疫試劑盒
  • 貨號：
    CSB-E06925Rb
  • 規格：
    96T/48T
  • 價格：
    ￥3800/￥2500
  • 其他：

產品詳情

  • 產品描述：
    兔子γ干擾素(IFN-γ)酶聯免疫試劑盒（CSB-E06925Rb）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的IFNG含量。IFNG 即干擾素 -γ，在免疫調節中發揮關鍵作用，可增強巨噬細胞活性、促進 Th1 細胞分化、調節 MHC 分子表達等。研究機制上，它通過與受體結合激活下游信號通路，調節眾多基因表達，在抗腫瘤、抗感染及自身免疫病中是重要研究靶點。試劑盒檢測范圍為62.5 pg/mL-4000 pg/mL，該產品適用于多種科研場景，包括但不限于動物免疫機制研究、疫苗效力評估實驗、感染性疾病模型中的免疫應答分析以及炎癥相關信號通路研究。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
  • 別名：
    IFNG ELISA Kit; Interferon gamma ELISA Kit; IFN-gamma ELISA Kit
  • 縮寫：
  • Uniprot No.：
  • 種屬：
    Oryctolagus cuniculus (Rabbit)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測范圍：
    62.5 pg/mL-4000 pg/mL
  • 靈敏度：
    15.6 pg/mL
  • 反應時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領域：
    Immunology
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rabbit IFN-γ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1Average %96
    Range %85-105
    1:2Average %94
    Range %85-105
    1:4Average %96
    Range %92-100
    1:8Average %95
    Range %83-105
  • 回收率：
    The recovery of rabbit IFN-γ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9585-100
    EDTA plasma (n=4)9585-105
  • 標準曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    40002.862 2.713 2.788 2.673
    20002.214 2.229 2.222 2.107
    10001.373 1.397 1.385 1.270
    5000.755 0.731 0.743 0.628
    2500.446 0.432 0.439 0.324
    1250.263 0.275 0.269 0.154
    62.50.202 0.194 0.198 0.083
    00.117 0.113 0.115 　
  • 數據處理：
  • 貨期：
    3-5 working days

產品評價

靶點詳情

  • 功能：
    Type II interferon produced by immune cells such as T-cells and NK cells that plays crucial roles in antimicrobial, antiviral, and antitumor responses by activating effector immune cells and enhancing antigen presentation. Primarily signals through the JAK-STAT pathway after interaction with its receptor IFNGR1 to affect gene regulation. Upon IFNG binding, IFNGR1 intracellular domain opens out to allow association of downstream signaling components JAK2, JAK1 and STAT1, leading to STAT1 activation, nuclear translocation and transcription of IFNG-regulated genes. Many of the induced genes are transcription factors such as IRF1 that are able to further drive regulation of a next wave of transcription. Plays a role in class I antigen presentation pathway by inducing a replacement of catalytic proteasome subunits with immunoproteasome subunits. In turn, increases the quantity, quality, and repertoire of peptides for class I MHC loading. Increases the efficiency of peptide generation also by inducing the expression of activator PA28 that associates with the proteasome and alters its proteolytic cleavage preference. Up-regulates as well MHC II complexes on the cell surface by promoting expression of several key molecules such as cathepsins B/CTSB, H/CTSH, and L/CTSL. Participates in the regulation of hematopoietic stem cells during development and under homeostatic conditions by affecting their development, quiescence, and differentiation.
  • 亞細胞定位：
    Secreted.
  • 蛋白家族：
    Type II (or gamma) interferon family
  • 組織特異性：
    Released primarily from activated T lymphocytes.
  • 數據庫鏈接：


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