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Rabbit Complement 3,C3 ELISA Kit

  • 中文名稱:
    兔補(bǔ)體蛋白3(C3)酶聯(lián)免疫試劑盒
  • 貨號:
    CSB-E08668Rb
  • 規(guī)格:
    96T/48T
  • 價格:
    ¥4200/¥3000
  • 其他:

產(chǎn)品詳情

  • 產(chǎn)品描述:
    兔補(bǔ)體蛋白3(C3)酶聯(lián)免疫試劑盒(CSB-E08668Rb)為競爭法ELISA試劑盒,定量檢測血清、血漿樣本中的C3含量。C3作為靶點在生物醫(yī)學(xué)領(lǐng)域受關(guān)注。它是補(bǔ)體系統(tǒng)關(guān)鍵成分。研究表明,C3參與免疫反應(yīng)與炎癥進(jìn)程,異常激活影響多種疾病發(fā)展。針對其的研究機(jī)制聚焦在阻斷異常激活途徑,期望開發(fā)出能治療相關(guān)疾病的療法。試劑盒檢測范圍為27.3 ng/mL-7000 ng/mL,適用于兔類動物模型中的免疫調(diào)控研究,如感染性疾病機(jī)制探索、自身免疫病理分析或炎癥相關(guān)藥物開發(fā)中的生物標(biāo)志物檢測,為科研人員提供標(biāo)準(zhǔn)化的實驗工具。本品僅用于科研,不用于臨床診斷,產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名:
    AHUS5, ARMD9, ASP, CPAMD1, acylation-stimulating protein cleavage product|complement component C3
  • 縮寫:
  • 種屬:
    Oryctolagus cuniculus (Rabbit)
  • 樣本類型:
    serum, plasma
  • 檢測范圍:
    27.3 ng/mL-7000 ng/mL
  • 靈敏度:
    27.3 ng/mL
  • 反應(yīng)時間:
    1-5h
  • 樣本體積:
    50-100ul
  • 檢測波長:
    450 nm
  • 研究領(lǐng)域:
    Immunology
  • 測定原理:
    quantitative
  • 測定方法:
    Competitive
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of rabbit C3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:1000Average %97
    Range %91-110
    1:2000Average %89
    Range %82-99
    1:4000Average %95
    Range %85-99
    1:8000Average %91
    Range %87-98
  • 回收率:
    The recovery of rabbit C3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9382-97
    EDTA plasma (n=4)9787-100
  • 標(biāo)準(zhǔn)曲線:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/mlOD1OD2Average
    70000.196 0.189 0.193
    17500.378 0.372 0.375
    437.50.812 0.805 0.809
    109.41.492 1.501 1.497
    27.32.012 2.003 2.008
    02.913 2.897 2.905
  • 數(shù)據(jù)處理:
  • 貨期:
    3-5 working days

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相關(guān)問答

 常見問題解答
Q:

What is the standard in the Rabbit Complement 3 ELISA kit?
code CSB-E08668Rb. I want to know if it is actually rabbit complement C3. If you could get me a molecular weight of the standard, that will be helpful. I need to be able to convert between mg/ml and molarity for my assay and to do that I need the molecular weight of the protein. NO sequence needed, just a weight in kD.

A:
Thanks for your inquiry.
CSB-E08668Rb
Standard is purified crude extract from native samples.
We don't suggest you convert the unit. Pls kindly follow the unit in the manual.
If you want to convert it, you can refer to: https://www.uniprot.org/uniprot/P12247.
Pls let me know if you have any further questions. Thank you.


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