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Rabbit Collagen Type Ⅱ,Col Ⅱ ELISA Kit

  • 中文名稱:
    兔子Ⅱ型膠原(Col Ⅱ)酶聯(lián)免疫試劑盒
  • 貨號(hào):
    CSB-E06856Rb
  • 規(guī)格:
    96T/48T
  • 價(jià)格:
    ¥4200/¥3000
  • 其他:

產(chǎn)品詳情

  • 產(chǎn)品描述:
    兔子Ⅱ型膠原(Col Ⅱ)酶聯(lián)免疫試劑盒(CSB-E06856Rb)為雙抗夾心法ELISA試劑盒,定量檢測(cè)血清、血漿樣本中的Col 含量。試劑盒檢測(cè)范圍為6.25 ng/mL-400 ng/mL,適用于科研場(chǎng)景中軟骨相關(guān)疾病模型研究,如關(guān)節(jié)炎病理機(jī)制探索、軟骨損傷修復(fù)評(píng)估或藥物干預(yù)效果驗(yàn)證等領(lǐng)域,為組織工程、生物材料開發(fā)及退行性疾病研究提供可靠的檢測(cè)工具本品僅用于科研,不用于臨床診斷,產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名:
    Collagen Type 2,Col 2,_x000D_ Collagen Type II,Col II
  • 縮寫:
    Col
  • 種屬:
    Oryctolagus cuniculus (Rabbit)
  • 樣本類型:
    serum, plasma
  • 檢測(cè)范圍:
    6.25 ng/mL-400 ng/mL
  • 靈敏度:
    1.56 ng/mL
  • 反應(yīng)時(shí)間:
    1-5h
  • 樣本體積:
    50-100ul
  • 檢測(cè)波長(zhǎng):
    450 nm
  • 研究領(lǐng)域:
    Signal Transduction
  • 測(cè)定原理:
    quantitative
  • 測(cè)定方法:
    Sandwich
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of rabbit Col Ⅱ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)  
    1:1 Average % 98  
    Range % 90-102  
    1:2 Average % 107  
    Range % 97-111  
    1:4 Average % 88  
    Range % 82-92  
    1:8 Average % 98  
    Range % 91-102  
  • 回收率:
    The recovery of rabbit Col Ⅱ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 90 82-94  
    EDTA plasma (n=4) 102 92-107  
                 
                 
  • 標(biāo)準(zhǔn)曲線:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average Corrected  
    400 2.576 2.528 2.552 2.457  
    200 1.949 1.986 1.968 1.873  
    100 1.421 1.366 1.394 1.299  
    50 0.827 0.811 0.819 0.724  
    25 0.434 0.417 0.426 0.331  
    12.5 0.314 0.302 0.308 0.213  
    6.25 0.234 0.221 0.228 0.133  
    0 0.093 0.097 0.095    
  • 數(shù)據(jù)處理:
  • 貨期:
    3-5 working days

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