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Pig transforming growth factor β1(TGF-β1)ELISA Kit

  • 中文名稱:
    豬轉化生長因子β1(TGF-β1)酶聯免疫試劑盒
  • 貨號:
    CSB-E06843p
  • 規格:
    96T/48T
  • 價格:
    ¥4200/¥3000
  • 其他:

產品詳情

  • 產品描述:
    豬轉化生長因子β1(TGF-β1)酶聯免疫試劑盒(CSB-E06843p)為雙抗夾心法ELISA試劑盒,定量檢測血清、血漿樣本中的TGFB1含量。TGFB1是重要的細胞因子,在胚胎發育、組織修復與再生等生理過程中發揮關鍵作用。其異常激活與腫瘤、纖維化疾病等多種病理狀態密切相關。研究發現它可通過多種信號通路調控細胞生長、分化和遷移,是極具潛力的治療靶點。試劑盒檢測范圍為0.78 ng/mL-50 ng/mL,適用于畜牧科研、動物模型構建、疫苗效力評估等場景,例如通過分析實驗性感染、營養干預或藥物處理后豬體內TGF-β1的濃度變化,揭示其調控機制或疾病關聯性本品僅用于科研,不用于臨床診斷,產品具體參數及操作步驟詳見產品說明書。
  • 別名:
    TGFB1 ELISA Kit; Transforming growth factor beta-1 proprotein [Cleaved into: Latency-associated peptide ELISA Kit; LAP); Transforming growth factor beta-1 ELISA Kit; TGF-beta-1)] ELISA Kit
  • 縮寫:
  • Uniprot No.:
  • 種屬:
    Sus scrofa (Pig)
  • 樣本類型:
    serum, plasma
  • 檢測范圍:
    0.78 ng/mL-50 ng/mL
  • 靈敏度:
    0.195 ng/mL
  • 反應時間:
    1-5h
  • 樣本體積:
    50-100ul
  • 檢測波長:
    450 nm
  • 研究領域:
    Signal Transduction
  • 測定原理:
    quantitative
  • 測定方法:
    Sandwich
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse TGF-β1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)  
    1:1 Average % 99  
    Range % 94-104  
    1:2 Average % 89  
    Range % 83-97  
    1:4 Average % 92  
    Range % 87-97  
    1:8 Average % 92  
    Range % 87-96  
  • 回收率:
    The recovery of mouse TGF-β1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 92 87-99  
    EDTA plasma (n=4) 96 90-100  
                 
                 
  • 標準曲線:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average Corrected  
    50 2.654 2.636 2.645 2.515  
    25 2.318 2.455 2.387 2.257  
    12.5 1.756 1.886 1.821 1.691  
    6.25 0.997 1.001 0.999 0.869  
    3.12 0.542 0.512 0.527 0.397  
    1.56 0.301 0.309 0.305 0.175  
    0.78 0.228 0.238 0.233 0.103  
    0 0.129 0.131 0.130    
  • 數據處理:
  • 貨期:
    3-5 working days

引用文獻

產品評價

靶點詳情

  • 功能:
    Transforming growth factor beta-1 proprotein: Precursor of the Latency-associated peptide (LAP) and Transforming growth factor beta-1 (TGF-beta-1) chains, which constitute the regulatory and active subunit of TGF-beta-1, respectively.; Required to maintain the Transforming growth factor beta-1 (TGF-beta-1) chain in a latent state during storage in extracellular matrix. Associates non-covalently with TGF-beta-1 and regulates its activation via interaction with 'milieu molecules', such as LTBP1, LRRC32/GARP and LRRC33/NRROS, that control activation of TGF-beta-1. Interaction with LRRC33/NRROS regulates activation of TGF-beta-1 in macrophages and microglia. Interaction with LRRC32/GARP controls activation of TGF-beta-1 on the surface of activated regulatory T-cells (Tregs). Interaction with integrins (ITGAV:ITGB6 or ITGAV:ITGB8) results in distortion of the Latency-associated peptide chain and subsequent release of the active TGF-beta-1.; Multifunctional protein that regulates the growth and differentiation of various cell types and is involved in various processes, such as normal development, immune function, microglia function and responses to neurodegeneration. Activation into mature form follows different steps: following cleavage of the proprotein in the Golgi apparatus, Latency-associated peptide (LAP) and Transforming growth factor beta-1 (TGF-beta-1) chains remain non-covalently linked rendering TGF-beta-1 inactive during storage in extracellular matrix. At the same time, LAP chain interacts with 'milieu molecules', such as LTBP1, LRRC32/GARP and LRRC33/NRROS that control activation of TGF-beta-1 and maintain it in a latent state during storage in extracellular milieus. TGF-beta-1 is released from LAP by integrins (ITGAV:ITGB6 or ITGAV:ITGB8): integrin-binding to LAP stabilizes an alternative conformation of the LAP bowtie tail and results in distortion of the LAP chain and subsequent release of the active TGF-beta-1. Once activated following release of LAP, TGF-beta-1 acts by binding to TGF-beta receptors (TGFBR1 and TGFBR2), which transduce signal. While expressed by many cells types, TGF-beta-1 only has a very localized range of action within cell environment thanks to fine regulation of its activation by Latency-associated peptide chain (LAP) and 'milieu molecules'. Plays an important role in bone remodeling: acts as a potent stimulator of osteoblastic bone formation, causing chemotaxis, proliferation and differentiation in committed osteoblasts. Can promote either T-helper 17 cells (Th17) or regulatory T-cells (Treg) lineage differentiation in a concentration-dependent manner. At high concentrations, leads to FOXP3-mediated suppression of RORC and down-regulation of IL-17 expression, favoring Treg cell development. At low concentrations in concert with IL-6 and IL-21, leads to expression of the IL-17 and IL-23 receptors, favoring differentiation to Th17 cells. Stimulates sustained production of collagen through the activation of CREB3L1 by regulated intramembrane proteolysis (RIP). Mediates SMAD2/3 activation by inducing its phosphorylation and subsequent translocation to the nucleus. Can induce epithelial-to-mesenchymal transition (EMT) and cell migration in various cell types.
  • 基因功能參考文獻:
    1. TGF-beta1 is activated or inactivated by MMP20 or KLK4 and that the amelogenin cleavage product is necessary for the in-solution mobility of TGF-beta1. PMID: 27633089
    2. Activated TGF-beta signaling rescued miR-143-reduced FSHR and intracellular signaling molecules, and miR-143-induced porcine granulosa cell apoptosis. PMID: 27882941
    3. TGF-beta1-induced epithelial-myofibroblast plasticity is FAK- dependent, whereas TGF-beta1-induced apoptosis is favored when FAK signaling is inhibited. PMID: 28360109
    4. this study shows that TGF-beta1 protects intestinal integrity and influences Smad and MAPK signal pathways in intestinal epithelium cells after TNF-alpha challenge PMID: 28142299
    5. this study shows that anemonin may ameliorate LPS-induced intestinal injury and improve restoration by regulating the TGF-b1 and EGFR signaling pathways PMID: 27189428
    6. The results indicated that TGF-beta-1 was associated with the restoration of intestinal morphology and barrier function following weaning stress. PMID: 25170924
    7. Data (including data from in vitro and in vivo experiments) suggest that day 14 elongated conceptus secretes proteins that up-regulate TGFbeta1 mRNA and TGFbeta1 expression in endometrium; TGFbeta1 may be important during pregnancy maintenance. PMID: 24042214
    8. The effects of semen, spermatozoa in extender, or extender alone on the expression of TGF-beta1, IL-10, and IL-6 in ovarian follicles are reported. PMID: 24020676
    9. Dietary (1,3/1,6)-beta-D-glucan reduced the mRNA expression of transforming growth factor (TGF) beta2 and tended to reduce the mRNA expression of TGF-beta1 in lung tissue of neonatal piglet. PMID: 23602250
    10. TGF-beta1, via TGF-beta1 receptor I and p38 MAPK signaling, reduces CFTR expression to impair CFTR-mediated anion secretion, which would likely compound the effects associated with mild CFTR mutations and ultimately would compromise male fertility. PMID: 23903699
    11. High yield isolation of BMP-2 from bone and in vivo activity of a combination of BMP-2/TGF-beta1. PMID: 22927042
    12. Boar seminal plasma contained TGF- beta1 and IL-10 but with high individual variation. PMID: 22482797
    13. The present study was aimed to determine the association between metalloproteinase 3 (MMP3), transforming growth factor beta 1 (TGFbeta1) and collagen type X alpha I (COL10A1) gene polymorphisms with traits related to leg weakness in pigs. PMID: 21739142
    14. Inhibition of transforming growth factor beta-1 augments liver regeneration after partial portal vein ligation in a porcine experimental model. PMID: 22251543
    15. TGF-beta prevents excessive heart valve growth under normal physiological conditions while it promotes cell proliferation in the early stages of repair. PMID: 21851806
    16. Crystals of dimeric porcine proTGF-beta1 reveal a ring-shaped complex, a novel fold for the prodomain, and show how the prodomain shields the growth factor from recognition by receptors and alters its conformation. PMID: 21677751
    17. The effects of different polymorphisms of TGF-beta1 on litter size in Large white pigs are reported. PMID: 20088852
    18. TGF-beta disrupts an IGF-II-stimulated autocrine amplification cascade that is necessary for muscle differentiation in vitro. PMID: 21106882
    19. beta-Catenin plays a critical role in mediating TGF-beta1-induced myofibroblast differentiation in aortic valve interstitial cells. PMID: 21127288
    20. Results suggest that Rac1 and RhoA are regulated by TGFbeta1 in the process of endothelial tube formation in collagen I gels. PMID: 16978608
    21. TGFbeta may play a role in the overall process of luteinization, but it appears not to influence steroidogenesis in luteinizing pig follicles. PMID: 20378284
    22. Elevated circulating TGF-beta(1) causes endothelial dysfunction through NADPH oxidase activation-induced oxidative stress, accelerating atherosclerosis and hypertension in apoE(-/-) mice. PMID: 20511416
    23. TGFbeta stimulation of ANK expression is dependent upon the influx of phosphate and calcium into ATDC5 cells at all stages of differentiation. PMID: 20432454
    24. Data suggest that focal adhesion kinase (FAK)-SMAD 2/3 mediate signal crosstalk between type II collagen and TGF-beta1 and regulate glycosaminoglycan secretion in chondrocytic cells. PMID: 19840848
    25. show that histone deacetylase 4 overexpression promotes TGF-beta1-induced synovium-derived stem cells chondrogenesis but inhibits chondrogenically differentiated stem cell hypertrophy. PMID: 19716643
    26. multiple effects of TGFbeta at the porcine conceptus-maternal interface, including integrin-mediated conceptus-maternal communication through LAP PMID: 15961561
    27. Repeated injection of adenovirus expressing porcine TGF-beta1 may be useful model for studying Peyronie's disease. PMID: 17651407
    28. identification of six quantitative trait loci for diabetes-related traits in F(2)-D Tgf/Tgf and F(2)-C Tgf/Tgf pigs PMID: 18160996
    29. FGF2 effectively blocks transforming growth factor-beta1 (TGF-beta1)-mediated myofibroblast activation PMID: 18218921
    30. Using the model of porcine aortic endothelial cells, we demonstrated that TGFbeta stimulates cellular spreading when cells are on collagen I; TGFbeta-stimulated Rac1-GTP accumulation, which was associated with increased MAPK p38 phosphorylation. PMID: 18387002
    31. Wounding activates valve interstitial cell (VICs), and TGF-beta signaling modulates VIC response to injury. PMID: 18832581
    32. Altered shear stress stimulates upregulation of endothelial VCAM-1 and ICAM-1 in a BMP-4- and TGF-beta1-dependent pathway. PMID: 19023092
    33. pTGF-beta1 induces human fibroblast synthesis & secretion of ET-1. TGF-beta1 activation of PI3K/AKT is not dependent on ET-1. ET-1 & TGF-beta1 independently promote fibroblast resistance to apoptosis through p38 MAPK & PI3K/AKT. PMID: 19188658
    34. transforming growth factor beta has a role in the regulation of thyroid function and growth [review] PMID: 19645615

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  • 亞細胞定位:
    [Latency-associated peptide]: Secreted, extracellular space, extracellular matrix.; [Transforming growth factor beta-1]: Secreted.
  • 蛋白家族:
    TGF-beta family
  • 數據庫鏈接:

    KEGG: ssc:397078

    STRING: 9823.ENSSSCP00000003267

    UniGene: Ssc.76



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