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  4. Pig terminal complement complex C5b-9 (TCC C5b-9)ELISA Kit

Pig terminal complement complex C5b-9 (TCC C5b-9)ELISA Kit

  • 中文名稱：
    豬末端補體復合物C5b-9(TCC C5b-9)酶聯免疫試劑盒
  • 貨號：
    CSB-E15837p
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥3600/￥2500
  • 其他：

產品詳情

  • 產品描述：
    豬末端補體復合物C5b-9(TCC C5b-9)酶聯免疫試劑盒（CSB-E15837p）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的TCC C5b-9含量。試劑盒檢測范圍為18.75 ng/mL-1200 ng/mL，適用于動物模型中的補體激活研究、炎癥性疾病機制探索或藥物干預效果評估等科研場景本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
  • 縮寫：
    TCC C5b-9
  • 種屬：
    Sus scrofa (Pig)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測范圍：
    18.75 ng/mL-1200 ng/mL
  • 靈敏度：
    4.69 ng/mL
  • 反應時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領域：
    Others
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of pig TCC C5b-9 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1Average %99
    Range %85-105
    1:2Average %95
    Range %85-113
    1:4Average %95
    Range %82-102
    1:8Average %97
    Range %85-110
  • 回收率：
    The recovery of pig TCC C5b-9 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9688-109
    EDTA plasma (n=4)9785-105
  • 標準曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/mlOD1OD2AverageCorrected
    12002.217 2.319 2.268 2.130
    6001.790 1.767 1.779 1.641
    3001.123 1.132 1.128 0.990
    1500.675 0.651 0.663 0.525
    750.397 0.403 0.400 0.262
    37.50.285 0.298 0.292 0.154
    18.750.231 0.216 0.224 0.086
    00.141 0.134 0.138 　
  • 數據處理：
  • 貨期：
    3-5 working days

產品評價

相關問答

■ 常見問題解答
Q:

At what wavelength do i read the results?

A:
Thanks for your inquiry.
CSB-E15837p
The detection wavelength must be 450nm.
The absorbance value will be very good at this wavelength. Considering there may be scratches at the bottom of the vial when you add samples, we suggest you use dual wavelength( detection wavelength and correction wavelength).
Pls let me know if you have any further questions. Thank you.


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