Pig neutrophil gelatinase-associated lipocalin(NGAL)ELISA Kit

文獻(xiàn)引用 (2) 說(shuō)明書(shū)下載

產(chǎn)品詳情

產(chǎn)品描述：

豬中性粒細(xì)胞明膠酶相關(guān)脂質(zhì)運(yùn)載蛋白(NGAL)酶聯(lián)免疫試劑盒（CSB-E16507p）為競(jìng)爭(zhēng)法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿樣本中的NGAL含量。試劑盒檢測(cè)范圍為7.8 pg/mL-500 pg/mL，可廣泛應(yīng)用于豬模型中的炎癥應(yīng)答機(jī)制研究、腎功能損傷評(píng)估及藥物干預(yù)效果分析等領(lǐng)域，能為獸醫(yī)病理學(xué)、畜牧科學(xué)及比較醫(yī)學(xué)領(lǐng)域的科研工作者提供可靠工具，助力于動(dòng)物疾病模型的建立與新型治療策略開(kāi)發(fā)。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書(shū)。
縮寫(xiě)：

NGAL
Uniprot No.：

F1RRX1
種屬：

Sus scrofa (Pig)
樣本類(lèi)型：

serum, plasma, tissue homogenates
檢測(cè)范圍：

7.8 pg/mL-500 pg/mL
靈敏度：

1.95 pg/mL
反應(yīng)時(shí)間：

1-5h
樣本體積：

50-100ul
檢測(cè)波長(zhǎng)：

450 nm
研究領(lǐng)域：

Metabolism
測(cè)定原理：

quantitative
測(cè)定方法：

Competitive

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

線(xiàn)性度：

To assess the linearity of the assay, samples were spiked with high concentrations of pig NGAL in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:200	Average %	105
	Range %	101-108
1:400	Average %	96
	Range %	92-98
1:800	Average %	96
	Range %	90-99
1:1600	Average %	89
	Range %	84-93

回收率：

The recovery of pig NGAL spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	91	87-94
EDTA plasma (n=4)	99	99-103

標(biāo)準(zhǔn)曲線(xiàn)：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.