Pig matrix metalloproteinase 9,MMP-9 ELISA Kit
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中文名稱:豬基質(zhì)金屬蛋白酶9(MMP-9)酶聯(lián)免疫試劑盒
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貨號(hào):CSB-E06798p
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規(guī)格:96T/48T
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價(jià)格:¥4200/¥3000
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其他:
產(chǎn)品詳情
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產(chǎn)品描述:豬基質(zhì)金屬蛋白酶9(MMP-9)酶聯(lián)免疫試劑盒(CSB-E06798p)為雙抗夾心法ELISA試劑盒,定量檢測(cè)血清、血漿、組織勻漿樣本中的MMP-9含量。MMP-9是一種重要靶點(diǎn)。它在機(jī)體生理與病理過(guò)程中發(fā)揮關(guān)鍵作用。在腫瘤中,它能促進(jìn)腫瘤細(xì)胞的侵襲與轉(zhuǎn)移。研究機(jī)制多圍繞其對(duì)細(xì)胞外基質(zhì)的降解、信號(hào)通路的調(diào)控等方面展開,對(duì)其深入研究有助于開發(fā)新的治療策略。試劑盒檢測(cè)范圍為0.625 ng/mL-40 ng/mL,該產(chǎn)品適用于動(dòng)物模型研究,例如炎癥性疾病機(jī)制探索、創(chuàng)傷修復(fù)過(guò)程分析或與MMP - 9相關(guān)的病理狀態(tài)評(píng)估,可為科研人員提供穩(wěn)定可靠的定量數(shù)據(jù)支持,但不可用于臨床診斷或其他非科研用途。本品僅用于科研,不用于臨床診斷,產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書。
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縮寫:MMP-9
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Uniprot No.:
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種屬:Sus scrofa (Pig)
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樣本類型:serum, plasma, tissue homogenates
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檢測(cè)范圍:0.625 ng/mL-40 ng/mL
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靈敏度:0.156 ng/mL
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反應(yīng)時(shí)間:1-5h
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樣本體積:50-100ul
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檢測(cè)波長(zhǎng):450 nm
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研究領(lǐng)域:Cancer
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測(cè)定原理:quantitative
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測(cè)定方法:Sandwich
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精密度:
Intra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess. -
線性度:
To assess the linearity of the assay, samples were spiked with high concentrations of pig MMP-9 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. Sample Serum(n=4) 1:1 Average % 96 Range % 85-106 1:2 Average % 95 Range % 91-99 1:4 Average % 96 Range % 88-105 1:8 Average % 95 Range % 90-102 -
回收率:
The recovery of pig MMP-9 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 96 87-100 EDTA plasma (n=4) 97 93-102 -
標(biāo)準(zhǔn)曲線:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. ng/ml OD1 OD2 Average Corrected 40 2.505 2.551 2.528 2.411 20 2.313 2.328 2.321 2.204 10 1.786 1.779 1.783 1.666 5 1.112 1.131 1.122 1.005 2.5 0.574 0.589 0.582 0.465 1.25 0.364 0.374 0.369 0.252 0.625 0.242 0.253 0.248 0.131 0 0.115 0.119 0.117 -
數(shù)據(jù)處理:
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貨期:3-5 working days
引用文獻(xiàn)
- Glycocalyx Degradation after Pulmonary Transplantation Surgery Rancan L.et al,european surgical research,2018
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