Pig matrix metalloproteinase 2/Gelatinase A,MMP-2 ELISA kit
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中文名稱：豬基質(zhì)金屬蛋白酶2/明膠酶A(MMP-2/Gelatinase A)酶聯(lián)免疫試劑盒
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貨號(hào)：CSB-E12842p
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規(guī)格：96T/48T
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價(jià)格：￥4200/￥3000
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其他：
產(chǎn)品詳情
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產(chǎn)品描述：豬基質(zhì)金屬蛋白酶2/明膠酶A(MMP-2/Gelatinase A)酶聯(lián)免疫試劑盒（CSB-E12842p）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織培養(yǎng)上清液、組織勻漿樣本中的MMP2/Gelatinase A含量。MMP2/Gelatinase A即基質(zhì)金屬蛋白酶2，在細(xì)胞外基質(zhì)代謝、組織重塑等生理過(guò)程中發(fā)揮重要作用。異常表達(dá)與腫瘤侵襲轉(zhuǎn)移、心血管疾病和關(guān)節(jié)炎等多種疾病相關(guān)。研究多聚焦其酶活性調(diào)控機(jī)制，尋找抑制其活性的靶點(diǎn)，為疾病治療提供新思路。試劑盒檢測(cè)范圍為47 ng/mL-3000 ng/mL，適用于科研領(lǐng)域探究豬模型中的組織再生機(jī)制、疾病相關(guān)分子通路及藥物干預(yù)效果評(píng)估等方向，為深入解析MMP - 2在生理病理中的功能提供高效檢測(cè)方案本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書(shū)。
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縮寫(xiě)：MMP2/Gelatinase A
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Uniprot No.：
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種屬：Sus scrofa (Pig)
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樣本類(lèi)型：serum, plasma, cell culture supernates, tissue homogenates
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檢測(cè)范圍：47 ng/mL-3000 ng/mL
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靈敏度：11.75 ng/mL
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反應(yīng)時(shí)間：1-5h
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樣本體積：50-100ul
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檢測(cè)波長(zhǎng)：450 nm
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研究領(lǐng)域：Cancer
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測(cè)定原理：quantitative
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測(cè)定方法：Sandwich
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精密度：
Intra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess. -
線性度：
To assess the linearity of the assay, samples were spiked with high concentrations of pig MMP-2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. Sample Serum(n=4) 1:1 Average % 94 Range % 92-97 1:2 Average % 103 Range % 99-105 1:4 Average % 93 Range % 89-98 1:8 Average % 92 Range % 87-99 -
回收率：
The recovery of pig MMP-2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 95 91-97 EDTA plasma (n=4) 90 86-93 -
標(biāo)準(zhǔn)曲線：
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. 
ng/ml OD1 OD2 Average Corrected 3000 2.437 2.506 2.472 2.379 1500 1.988 1.966 1.977 1.884 750 1.565 1.646 1.606 1.513 375 1.163 1.259 1.211 1.118 187.5 0.774 0.764 0.769 0.676 94 0.352 0.367 0.360 0.267 47 0.222 0.214 0.218 0.125 0 0.096 0.089 0.093 -
數(shù)據(jù)處理：
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貨期：3-5 working days
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