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  4. Pig interleukin 8,IL-8 ELISA Kit

Pig interleukin 8,IL-8 ELISA Kit

  • 中文名稱：
    豬白介素8(IL-8/CXCL8)酶聯免疫試劑盒
  • 貨號：
    CSB-E06787p
  • 規格：
    96T/48T
  • 價格：
    ￥3600/￥2500
  • 其他：

產品詳情

  • 產品描述：
    豬白介素8(IL-8/CXCL8)酶聯免疫試劑盒（CSB-E06787p）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織培養上清液、組織勻漿樣本中的CXCL8含量。CXCL8，即CXC趨化因子配體8，也稱為白細胞介素-8，是一種重要的趨化因子，主要由免疫細胞和腫瘤細胞分泌。它通過激活中性粒細胞和其他免疫細胞，參與炎癥反應和腫瘤的發生發展。CXCL8的研究機制涉及與受體CXCR1和CXCR2的結合，以及下游信號通路的激活，如MAPK和PI3K/Akt通路。試劑盒檢測范圍為125 pg/mL-8000 pg/mL，靈敏度為31.25 pg/mL。適用于體外細胞因子分泌分析、感染或炎癥模型中免疫應答評估、疫苗研發過程中的免疫效果監測等科研場景本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
  • 別名：
    CXCL8 ELISA Kit; IL8Interleukin-8 ELISA Kit; IL-8 ELISA Kit; Alveolar macrophage chemotactic factor I ELISA Kit; AMCF-I ELISA Kit; C-X-C motif chemokine 8 ELISA Kit; Chemokine ELISA Kit; C-X-C motif) ligand 8 ELISA Kit
  • 縮寫：
  • Uniprot No.：
  • 種屬：
    Sus scrofa (Pig)
  • 樣本類型：
    serum, plasma, cell culture supernates, tissue homogenates
  • 檢測范圍：
    125 pg/mL-8000 pg/mL
  • 靈敏度：
    31.25 pg/mL
  • 反應時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領域：
    Immunology
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of pig IL-8 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:1 Average % 95  
    Range % 91-99  
    1:2 Average % 90  
    Range % 86-94  
    1:4 Average % 88  
    Range % 84-93  
    1:8 Average % 92  
    Range % 88-97  
  • 回收率：
    The recovery of pig IL-8 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 99 95-104  
    EDTA plasma (n=4) 85 81-90  
                 
                 
  • 標準曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average Corrected  
    8000 2.662 2.601 2.632 2.460  
    4000 2.215 2.161 2.188 2.016  
    2000 1.498 1.404 1.451 1.279  
    1000 0.871 0.819 0.845 0.673  
    500 0.510 0.507 0.509 0.337  
    250 0.380 0.359 0.370 0.198  
    125 0.271 0.261 0.266 0.094  
    0 0.174 0.170 0.172 　  
  • 數據處理：
  • 貨期：
    3-5 working days

產品評價

平均分:
5.0分 - 1 個評價

樣品類型：細胞培養上清液

樣品信息：其他

稀釋比：沒有稀釋

產品評價： 結構很好，已發文章

By 高老師

靶點詳情

  • 最新研究進展：
    IL8，也稱為白細胞介素-8，是一種細胞因子蛋白質，參與炎癥和免疫反應。它主要由激活的單核細胞和巨噬細胞產生，在炎癥、感染和損傷等條件下被釋放。IL8作為趨化因子，可以引導白細胞移動到感染或受損組織，促進炎癥反應和免疫細胞的增殖和激活。最近的研究表明，IL8也參與許多其他生物學過程，如細胞增殖、生存、轉移和血管新生等。
  • 功能：
    IL-8 is a chemotactic factor that attracts neutrophils, basophils, and T-cells, but not monocytes. It is also involved in neutrophil activation. It is released from several cell types in response to an inflammatory stimulus.
  • 基因功能參考文獻：
    1. This study evaluated the subcellular localization and other functions of TGEV nsp7 protein through analysis of its effects on cell growth, cell cycle progression, interleukin 8 (IL-8) expression, and NF-kappaB activation. PMID: 29521102
    2. The JNK-activated AP-1 subunit c-Jun was critical for the up-regulation of IL-8 expression by PRRSV PMID: 28347884
    3. These results suggest that porcine circovirus 2 induces IL-8 secretion via the TLR2/MyD88/NF-kappaB signalling pathway. PMID: 26581603
    4. Facilitated degradation of the RGS16 by porcine circovirus type 2 ORF3 further enhances NFkappaB translocation into the nucleus through the ERK1/2 signalling pathway and increased IL-6 and IL-8 mRNA transcripts. PMID: 25575706
    5. Suggest that the interplay between TGFbeta-2 and LPS regulates the levels of IL-8 in the immature newborn intestine. PMID: 25147235
    6. downregulated gene expression elite controllers (fraction of human population displaying resistance to HIV infection) PMID: 23751822
    7. Data suggest that MSK1 and MSK2 are the major CREB kinases in fibroblast-like synoviocytes from rheumatoid arthritis patients stimulated with lysophosphatidic acid and that phosphorylation of CREB1 at Ser-133 plays a significant role in IL-8 production. PMID: 24792438
    8. Study presents evidence demonstrating a single species of exotoxin ApxI, derived from A. pleuropneumoniae serotype 10, induces the expression and production of proinflammatory cytokines IL-1beta, IL-8 and TNF-alpha in porcine alveolar macrophages. PMID: 21314908
    9. local expression of IL-1beta and IL-8 in non-bacterial thrombotic endocarditis, Staphylococcus aureus infective endocarditis, animals with S. aureus sepsis without endocarditis and controls PMID: 23879680
    10. In swine, IL-8, TNF-ALPHA, INOS AND MIP-1BETA were increased during mechanical ventilation in a time-related fashion. PMID: 23102097
    11. Classical swine fever virus NS2 protein promotes interleukin-8 expression. PMID: 21318239
    12. Developmental changes in IL-8 isoforms may serve to minimize its inflammatory effects in the fetus and also provide a mechanism to restore its full activity after birth PMID: 19232500

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  • 亞細胞定位：
    Secreted.
  • 蛋白家族：
    Intercrine alpha (chemokine CxC) family
  • 組織特異性：
    Alveolar macrophages.
  • 數據庫鏈接：

    KEGG: ssc:396880

    STRING: 9823.ENSSSCP00000026976

    UniGene: Ssc.658



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