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Pig inducible nitric oxide synthase(iNOS)ELISA Kit

  • 中文名稱:
    豬誘導型一氧化氮合成酶(iNOS)酶聯免疫試劑盒
  • 貨號:
    CSB-E17489p
  • 規格:
    96T/48T
  • 價格:
    ¥4200/¥3000
  • 其他:

產品詳情

  • 產品描述:
    豬誘導型一氧化氮合成酶(iNOS)酶聯免疫試劑盒(CSB-E17489p)為雙抗夾心法ELISA試劑盒,定量檢測血清、血漿、組織勻漿樣本中的iNOS含量。iNOS即誘導型一氧化氮合酶,在炎癥、免疫反應等生理病理過程中發揮重要作用。其背景在于它可被多種細胞因子和脂多糖等誘導表達。研究機制主要聚焦于其調控NO生成,過量NO與組織損傷、腫瘤等疾病相關,是潛在治療靶點。試劑盒檢測范圍為0.05 U/mL-40 U/mL,適用于動物疾病模型構建、免疫調節機制研究、感染性疾病病理分析等領域,為探究炎癥相關疾病的分子機制、評估實驗性治療方案的調控效果提供可靠工具,滿足科研人員對iNOS動態變化的定量分析需求。本品僅用于科研,不用于臨床診斷,產品具體參數及操作步驟詳見產品說明書。
  • 別名:
    Nos2 ELISA Kit; InoslNitric oxide synthase ELISA Kit; inducible ELISA Kit; EC 1.14.13.39 ELISA Kit; Inducible NO synthase ELISA Kit; Inducible NOS ELISA Kit; iNOS ELISA Kit; Macrophage NOS ELISA Kit; MAC-NOS ELISA Kit; NOS type II ELISA Kit; Peptidyl-cysteine S-nitrosylase NOS2 ELISA Kit
  • 縮寫:
    iNOS
  • Uniprot No.:
  • 種屬:
    Sus scrofa (Pig)
  • 樣本類型:
    serum, plasma, tissue homogenates
  • 檢測范圍:
    0.05 U/mL-40 U/mL
  • 靈敏度:
    0.05 U/mL
  • 反應時間:
    1-5h
  • 樣本體積:
    50-100ul
  • 檢測波長:
    450 nm
  • 研究領域:
    Metabolism
  • 測定原理:
    quantitative
  • 測定方法:
    Sandwich
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<8%        
    Three samples of known concentration were tested twenty times on one plate to assess.    
    Inter-assay Precision (Precision between assays): CV%<10%        
    Three samples of known concentration were tested in twenty assays to assess.      
                   
  • 線性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of pig iNOS in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.  
      Sample Serum(n=4)    
    1:1 Average % 87    
    Range % 81-95    
    1:8 Average % 93    
    Range % 89-99    
    1:4 Average % 87    
    Range % 83-90    
    1:8 Average % 89    
    Range % 84-100    
  • 回收率:
    The recovery of pig iNOS spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.  
     
    Sample Type Average % Recovery Range    
    Serum (n=5) 92 86-100    
    EDTA plasma (n=4) 93 81-99    
                   
                   
  • 標準曲線:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.  
     
    U/ml OD1 OD2 Average Corrected    
    40 1.818 1.806 1.812 1.666    
    13.3 1.652 1.635 1.644 1.498    
    4.44 1.447 1.429 1.438 1.292    
    1.48 0.987 0.963 0.975 0.829    
    0.49 0.665 0.648 0.657 0.511    
    0.16 0.368 0.355 0.362 0.216    
    0.05 0.249 0.244 0.247 0.101    
    0 0.145 0.146 0.146      
  • 數據處理:
  • 貨期:
    3-5 working days

產品評價

靶點詳情

  • 功能:
    Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In macrophages, NO mediates tumoricidal and bactericidal actions. Also has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such PTGS2/COX2. As component of the iNOS-S100A8/9 transnitrosylase complex involved in the selective inflammatory stimulus-dependent S-nitrosylation of GAPDH implicated in regulation of the GAIT complex activity and probably multiple targets including ANXA5, EZR, MSN and VIM. Involved in inflammation, enhances the synthesis of proinflammatory mediators such as IL6 and IL8.
  • 基因功能參考文獻:
    1. these data show a role for iNOS-produced reactive oxygen species in maintaining homeostasis of the gut microbiota PMID: 29210363
    2. Results suggest that dopaminergic modulation of striatal function is altered in the iNOS KO mice. These alterations may be related to the decreased expression and activation of astrocytes and microglia in the iNOS KO mice. In conclusion, the phenotype profile of iNOS mutant mice corroborates iNOS constitutive function. PMID: 29751018
    3. The role of miR-294 and miR-721 in the regulation of NOS2 expression during Leishmania replication in infected macrophages pointing these miRNAs as potential new targets for drug development. PMID: 28276497
    4. Our results uncover the molecular mechanism behind the constricted regulation of Nos2 expression and open new therapeutic opportunities based on epigenetic activities of caspase-1 against infectious and inflammatory diseases. PMID: 28150715
    5. Data suggest that, in biocatalytic cycle of iNos, deferred ET (electron tunneling) from substrate or undue ET from/to cofactor leads to side products. These studies involved quantum mechanics, DFT (Density Functional Theory), thermodynamics, nondynamical electron correlation, and molecular modeling. PMID: 29407906
    6. iNOS-derived nitric oxide plays a role in telogen elongation under the inflammatory conditions associated with diabetes in mice. PMID: 29763605
    7. IFN-gamma-iNOS axis are an essential pathway in the pathogenesis of arenavirus hemorrhagic fever. PMID: 28826838
    8. Results demonstrate that TLR2 signal plays an important role in the regulation of iNOS expression after C. sinensis infection. TLR2 signal is also beneficial to limiting worm growth and development and contributing to the susceptibility to C. sinensis in which the iNOS/NO reactions possibly participate. PMID: 28784165
    9. Influence of 1mM MbetaCD on the fenoterol-driven changes in both contractility and NO level was strongly attenuated by inhibition of Gi-protein (pertussis toxin), Akt (Akt 1/2 kinase inhibitor) or NO-synthase (L-NAME)..Obtained results suggest that slight cholesterol depletion upregulates Gi-protein/Akt/NO-synthase signaling that attenuates the positive inotropic response to b2-adrenergic stimulation PMID: 27170493
    10. Diphenyleneiodonium selectively interacts with heme protein of iNOS, inhibiting nitric oxide production. PMID: 26880746
    11. omega-alkynyl arachidonic acid may promote the anti-inflammatory M2 polarization of macrophages in acute myocardial infarction via regulating the cross-talk between PKM2, HIF-1alpha and iNOS. PMID: 28964797
    12. Inducible nitric oxide synthase (NOS2)-deficiency enhanced survival and reduced tumor severity in genetically engineered mouse model of pancreatic cancer (KPC mouse). PMID: 27367029
    13. These results suggested that Fyn has a regulatory role in iNOS expression in astrocytes during neuroinflammatory responses. PMID: 29180007
    14. Inducible NO synthase (iNOS) mRNA levels were significantly increased immediately after exposure to hypergravity. PMID: 27174912
    15. Downregulation of inducible NO synthetase (iNOS) resulted in downregulation of heme oxygenase 1 (HO-1), and, conversely, upregulation of iNOS enhanced HO-1 activity. PMID: 27752990
    16. results indicate that in addition to its function in caveolae biogenesis, Cavin-2 plays a critical role in endothelial cell maintenance and function by regulating eNOS activity. PMID: 28912276
    17. iNOS is not involved in the cardioprotective effects of late-phase remote preconditioning of trauma. PMID: 26450997
    18. Data (including data from studies using knockout mice) suggest that expression of iNOS mRNA/protein is elevated in liver cytosol/mitochondria in sepsis; these changes are related to enhanced oxidative/nitrosative stress in liver in sepsis; absence of iNOS (but not nNOS) prevents impairment of liver mitochondria during sepsis; melatonin/antioxidant treatment prevents liver damage. PMID: 28110436
    19. PHLPP1 reduced IFN-gamma-stimulated but not LPS-induced ERK1/2 phosphorylation, and inhibition of ERK1/2 abolished IFN-gamma-induced ser(727) STAT1 phosphorylation and iNOS expression. PMID: 24443556
    20. this study shows that knockout of Nos2 in mice lacking Arginase1 ameliorates allergic contact hypersensitivity PMID: 28747341
    21. Aortic Nos2 levels were higher in Adamts1-deficient mice and in a mouse model of Marfan syndrome. PMID: 28067899
    22. iNOS plays a critical role in burn-induced Sirt1 S-nitrosylation and acetylation and activation of p65 NF-kappaB and p53 in mouse skeletal muscle. PMID: 28099528
    23. gammadelta T cells express NOS2 not only in vitro after t-cell receptor triggering, but also directly ex vivo. Nos2 deficient mice have fewer gammadelta T cells in peripheral lymph nodes than their wild-type counterparts, and these cells exhibit a reduced ability to produce IL-2. the inactivation of endogenous NOS2 significantly reduced gammadelta T cell proliferation and glycolysis metabolism that can be restored in ... PMID: 27812136
    24. this study shows that iNOS-derived oxidative stress plays a key role in psoriasis induced kidney dysfunction PMID: 28249219
    25. stability of LPS-induced iNOS mRNA was increased by GlcN under normal glucose conditions. These results suggest that GlcN regulates inflammation by sensing energy states of normal and fuel excess. PMID: 27927986
    26. this study shows that all-trans retinoic acid enhancement of neutrophil differentiation is iNOS-dependent PMID: 28189972
    27. H2S recruits iNOS to generate NO to inhibit high glucose-induced NOX4 expression, oxidative stress, and matrix protein accumulation in renal epithelial cells; the two gasotransmitters H2S and NO and their interaction may serve as therapeutic targets in diabetic kidney disease. PMID: 28188286
    28. Valine/Isoleucine mutation differentially affects catalytic steps in mammalian and bacterial nitric oxide synthases. PMID: 28074650
    29. M1-polarized macrophages significantly downregulated S1P4. The expression of the remaining three S1P receptors did not change. S1P increased expression of iNOS under M2-polarizing conditions. PMID: 28367448
    30. identify iNOS/NO as an integral component of IFN-beta production in response to dsRNA in hepatocytes in a pathway that involves the coordinated activities of TLR3/Trif and PKR PMID: 27226571
    31. The effect of thalidomide is induced by the inhibition of NOS enzyme predominantly iNOS. PMID: 27899254
    32. deletion or TNF-mediated restriction of Arg1 unleashes the production of nitric oxide by NOS2, which is critical for pathogen control. PMID: 27117406
    33. inhibition of inflammation increases lymphatic vessel density, decreases perilymphatic iNOS expression, increases lymphatic vessel pumping frequency, and restores lymphatic clearance of interstitial fluid to normal levels. PMID: 26796537
    34. The higher iNOS inhibition activity of the tested Schiff bases, relative to that of COX-2, seems to be a reflection of the combined suppressive effects exerted by their nalidixic acid, isatins (4a-c), and l-amino acid moieties against iNOS expression. PMID: 27092477
    35. elevated gene expression in allergic mice is downregulated with anti-interleukin 33 antibodies PMID: 26489077
    36. NOS2, while critical to the development of the acute inflammatory response to injury, is also necessary to control the late phase response to bleomycin. PMID: 26526764
    37. IFNgamma-dependent expression of NOS2 in the brain contributes to BBB breakdown and early mortality in murine PM. PMID: 26418460
    38. The role of NO and/or iNOS in rabies infection in mice and its effects on other immune molecules PMID: 26690069
    39. Inhibition of iNOS expression enhanced the therapeutic efficacy of alpha-galactosylceramide by increasing tumor antigen-specific immune response and the suppression of MDSCs. PMID: 26496031
    40. Amomum tsao-ko fruit extract suppresses lipopolysaccharide-induced inducible nitric oxide synthase by inducing heme oxygenase-1 in macrophages and in septic mice. PMID: 26852687
    41. Alcohol induces upregulation of iNOS in the intestine and iNOS signaling is required for muR-212 overexpression. PMID: 26207424
    42. In mice, Collagen-induced arthritis appears to increase the presence of iNOS in aorta, as well as in heart and in kidney microcirculation. PMID: 26456019
    43. iNOS activity controls inflammation, angiogenesis, and fibrogenesis in polyether-polyurethane synthetic implants. PMID: 26106257
    44. The findings demonstrated a substantial role of mitochondrial dysfunction in mediating the downregulation of NKCC2 and ENaCalpha in obstructive kidney disease, possibly via iNOS-derived nitric oxide and BNP. PMID: 26207612
    45. oxLDL induced iNOS expression inhibits macrophage-derived foam cell migration PMID: 24858340
    46. COX-2 is necessary to protect against tubular injury and apoptosis after unilateral ureteral obstruction but not necessary to protect against oxidative stress PMID: 26671967
    47. Glabridin downregulated iNOS mRNA expression and activity in LPS-stimulated macrophage-like cells under chronic glucose stress. PMID: 25737160
    48. Calmodulin (CaM) is essential but not indispensible for the assembly of iNOS and such CaM-free iNOS may help in elucidating the role of CaM on iNOS catalysis. PMID: 25822458
    49. novel roles for Ifngamma and Nos2 in regulating Actin, Tubulin, CD11b, motility and morphology during the aggregation response of adherent peritoneal exudate PMID: 26029930
    50. Data show that the activation of mammalian target of rapamycin complex mTORC1 during encephalomyocarditis virus infection is chemokine (C-C) receptor 5-dependent and promotes the translation of inducible NO synthase (iNOS) and cyclooxygenase (COX)-2. PMID: 26408666

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  • 亞細胞定位:
    Cytoplasm, cytosol.
  • 蛋白家族:
    NOS family
  • 組織特異性:
    Macrophages.
  • 數據庫鏈接:

    KEGG: mmu:18126

    STRING: 10090.ENSMUSP00000018610

    UniGene: Mm.2893



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