Pig glucagon like peptide 1(GLP1) ELISA Kit

產品詳情

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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產品描述：

豬胰高血糖素樣肽1(GLP1)酶聯免疫試劑盒（CSB-EQ027281PI）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿樣本中的GLP1含量。GLP1即胰高血糖素樣肽-1，是腸道L細胞分泌的一種肽類激素。它能促進胰島素分泌、抑制胰高血糖素分泌，延緩胃排空，從而降低血糖。目前基于GLP1的藥物在糖尿病、肥胖癥等疾病治療領域研究熱度高，為相關治療帶來新方向。試劑盒檢測范圍為15.6 pg/mL-1000 pg/mL，適用于基礎科研領域中對豬模型GLP1分泌調控機制的研究、代謝性疾病動物模型評估、以及相關生物活性分子或藥物干預效果的體外檢測，為探索GLP1在糖代謝、能量平衡及腸道 - 胰腺軸功能中的作用提供可靠工具本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
縮寫：

GLP1
種屬：

Sus scrofa (Pig)
樣本類型：

serum, plasma
檢測范圍：

15.6 pg/mL-1000 pg/mL
靈敏度：

3.9 pg/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Metabolism
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of pig GLP1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	85
	Range %	80-93
1:2	Average %	95
	Range %	90-105
1:4	Average %	98
	Range %	92-110
1:8	Average %	93
	Range %	85-98

回收率：

The recovery of pig GLP1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	89-104
EDTA plasma (n=4)	94	90-100

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.