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中文名稱:豬鈣衛蛋白(CALP)酶聯免疫試劑盒
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貨號:CSB-EQ013485PI
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規格:96T/48T
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價格:¥4200/¥3000
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其他:
產品詳情
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產品描述:豬鈣衛蛋白(CALP)酶聯免疫試劑盒(CSB-EQ013485PI)為競爭法ELISA試劑盒,定量檢測血清、血漿、組織勻漿樣本中的CALP含量。試劑盒檢測范圍為0.312 ng/mL-20 ng/mL,適用于動物疾病模型研究(如腸道炎癥、感染性疾病)、免疫機制探索或藥物開發中炎癥標志物的動態監測。適用于獸醫病理學、比較醫學及畜牧養殖領域的科研分析。本品僅用于科研,不用于臨床診斷,產品具體參數及操作步驟詳見產品說明書。
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縮寫:CALP
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種屬:Sus scrofa (Pig)
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樣本類型:serum, plasma, tissue homogenates
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檢測范圍:0.312 ng/mL-20 ng/mL
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靈敏度:0.078 ng/mL
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反應時間:1-5h
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樣本體積:50-100ul
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檢測波長:450 nm
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研究領域:Others
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測定原理:quantitative
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測定方法:Competitive
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精密度:
Intra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess. -
線性度:
To assess the linearity of the assay, samples were spiked with high concentrations of pig CALP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. Sample Serum(n=4) 1:100 Average % 96 Range % 91-102 1:200 Average % 85 Range % 81-89 1:400 Average % 103 Range % 98-107 1:800 Average % 87 Range % 83-92 -
回收率:
The recovery of pig CALP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 94 88-97 EDTA plasma (n=4) 106 102-108 -
標準曲線:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. ng/ml OD1 OD2 Average 20 0.099 0.101 0.100 10 0.160 0.168 0.164 5 0.241 0.255 0.248 2.5 0.365 0.373 0.369 1.25 0.544 0.556 0.550 0.625 0.713 0.755 0.734 0.312 0.984 1.044 1.014 0 1.765 1.834 1.800 -
數據處理:
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貨期:3-5 working days
引用文獻
- The generation and characterisation of a physiologically relevant porcine model of inflammatory bowel disease T Winogrodzki,/,2023
- TNF ΔARE pigs: a translational Crohn`s Disease model T Winogrodzki,Journal of Crohn's & colitis,2023
- Effects of nature identical essential oils (carvacrol, thymol and cinnamaldehyde) on growth performance of piglets and non-invasive markers of antioxidant status and calprotectin release R Rebucci,Livestock Science,2022
- The association between faecal host DNA or faecal calprotectin and feed efficiency in pigs fed yeast-enriched protein concentrate Slinger KR,Animal,2019
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