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  4. Pig brain natriuretic peptide,BNP ELISA Kit

Pig brain natriuretic peptide,BNP ELISA Kit

  • 中文名稱：
    豬腦鈉素/腦鈉尿肽(BNP)酶聯免疫試劑盒
  • 貨號：
    CSB-E06807p
  • 規格：
    96T/48T
  • 價格：
    ￥4200/￥3000
  • 其他：

產品詳情

  • 產品描述：
    豬腦鈉素/腦鈉尿肽(BNP)酶聯免疫試劑盒（CSB-E06807p）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的BNP含量。BNP即腦鈉肽，由心室肌細胞分泌。當心室容量負荷或壓力負荷增加時，其分泌增加。它具有利鈉、利尿、擴血管等作用，可拮抗腎素-血管緊張素-醛固酮系統。目前圍繞BNP開展多項研究，旨在利用其特性改善心臟功能及應對相關疾病。試劑盒檢測范圍為31.25 pg/mL-2000 pg/mL，適用于心血管疾病基礎研究、動物疾病模型評估、藥效學實驗中生物標志物動態監測等科研場景本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
  • 別名：
    NPPBNatriuretic peptides B ELISA kit; Gamma-brain natriuretic peptide) [Cleaved into: Brain natriuretic peptide 32 ELISA kit; BNP-32); Brain natriuretic peptide 26 ELISA kit; BNP-26)] ELISA kit
  • 縮寫：
    BNP
  • Uniprot No.：
  • 種屬：
    Sus scrofa (Pig)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測范圍：
    31.25 pg/mL-2000 pg/mL
  • 靈敏度：
    7.8 pg/mL
  • 反應時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領域：
    Cardiovascular
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of pig BNP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:1 Average % 90  
    Range % 86-95  
    1:2 Average % 94  
    Range % 89-98  
    1:4 Average % 85  
    Range % 80-93  
    1:8 Average % 98  
    Range % 92-102  
  • 回收率：
    The recovery of pig BNP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 95 91-102  
    EDTA plasma (n=4) 88 84-92  
                 
                 
  • 標準曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average Corrected  
    2000 2.551 2.501 2.526 2.392  
    1000 2.003 1.961 1.982 1.848  
    500 1.271 1.212 1.242 1.108  
    250 0.742 0.719 0.731 0.597  
    125 0.407 0.435 0.421 0.287  
    62.5 0.326 0.303 0.315 0.181  
    31.25 0.227 0.214 0.221 0.087  
    0 0.139 0.128 0.134 　  
  • 數據處理：
  • 貨期：
    3-5 working days

產品評價

靶點詳情

  • 功能：
    Cardiac hormone that plays a key role in mediating cardio-renal homeostasis. May also function as a paracrine antifibrotic factor in the heart. Acts by specifically binding and stimulating NPR1 to produce cGMP, which in turn activates effector proteins that drive various biological responses. Involved in regulating the extracellular fluid volume and maintaining the fluid-electrolyte balance through natriuresis, diuresis, vasorelaxation, and inhibition of renin and aldosterone secretion. Binds the clearance receptor NPR3.; Plays a key role in cardiovascular homeostasis through natriuresis, diuresis, vasorelaxation, and inhibition of renin and aldosterone secretion. In vivo, induces vasodilation.; May affect cardio-renal homeostasis. Able to promote the production of cGMP although its potency is very low compared to brain natriuretic peptide 32.
  • 基因功能參考文獻：
    1. CNP and BNP serve as oocyte maturation inhibitor to inhibit oocyte maturation in mammals PMID: 24912131
    2. ANP and BNP gene expression differs considerably between cardiac chambers in the first 72 hours of life in healthy piglets, resembling the transition from fetal to neonate circulation. The cardiac gene expression does not explain plasma concentrations. PMID: 23539511
    3. Up-regulation of BNP and a reduction in a ratio of sarcoplasmic reticulum calcium ATPase 2a in failing myocardium are observed in a porcine model of tachycardia. PMID: 20880599
    4. serum levels predict mortality in chest pain patients in Argentina PMID: 21958326
    5. Data show that all five molecules, BNP, ICAM-1, TNF-alpha, VCAM-1 and IL-6, quickly and reliably signaled adverse interactions. PMID: 21471537
    6. identification and characterization of a novel alternatively spliced variant of porcine and human NPPB resulting from exon 2 skipping PMID: 20660094
    7. Arial natriuretic factor (ANF) and N-terminal proANF may be better suited than brain natriuretic peptide as markers of cardiac preload during the development and treatment of acute heart failure. PMID: 19361150
    8. Regional differences in BNP and CNP expression were observed in myocardium with sustained left ventricular dyssynchronous contraction. PMID: 19540894

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  • 亞細胞定位：
    Secreted.; [Brain natriuretic peptide 32]: Secreted.
  • 蛋白家族：
    Natriuretic peptide family
  • 組織特異性：
    Heart atrium.; [Brain natriuretic peptide 32]: Brain (at protein level).; [Brain natriuretic peptide 26]: Brain (at protein level).
  • 數據庫鏈接：

    KEGG: ssc:396844

    STRING: 9823.ENSSSCP00000003722

    UniGene: Ssc.629



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