在线日韩日本国产亚洲丨少妇伦子伦情品无吗丨欧美性猛交xxxx免费看蜜桃丨精品人妻系列无码一区二区三区丨亚洲精品无码不卡在线播放

Your Good Partner in Biology Research

Pig Tumor necrosis factor (TNF/TNFA/TNFSF2) ELISA kit

  • 中文名稱:
    豬腫瘤壞死因子alpha(TNF/TNFA/TNFSF2)酶聯免疫試劑盒
  • 貨號:
    CSB-E16980p
  • 規格:
    96T/48T
  • 價格:
    ¥3600/¥2500
  • 其他:

產品詳情

  • 產品描述:
    豬腫瘤壞死因子alpha(TNF/TNFA/TNFSF2)酶聯免疫試劑盒(CSB-E16980p)為雙抗夾心法ELISA試劑盒,定量檢測血清、血漿、組織培養上清液、組織勻漿樣本中的TNF含量。TNF(腫瘤壞死因子)是一種重要的細胞因子。背景上,它在免疫調節、炎癥反應等生理過程中起關鍵作用。研究機制方面,TNF可與相應受體結合,激活多條信號通路,從而調控細胞的存活、增殖、凋亡等過程,其異常表達與多種疾病相關。試劑盒檢測范圍為0.047 ng/mL-3 ng/mL,靈敏度為0.040 ng/mL。該產品適用于科研領域中對豬免疫系統功能的探索,如評估病原體(如豬繁殖與呼吸綜合征病毒、豬圓環病毒等)感染模型中細胞因子風暴的動態變化,或研究抗炎藥物、免疫調節劑對TNFα表達的影響,為畜牧獸醫研究、動物疾病模型構建及疫苗研發提供可靠的數據支持。本品僅用于科研,不用于臨床診斷,產品具體參數及操作步驟詳見產品說明書。
  • 別名:
    TNF; TNFA; TNFSF2; Tumor necrosis factor; Cachectin; TNF-alpha; Tumor necrosis factor ligand superfamily member 2; TNF-a
  • 縮寫:
  • Uniprot No.:
  • 種屬:
    Sus scrofa (Pig)
  • 樣本類型:
    serum, plasma, cell culture supernates, tissue homogenates
  • 檢測范圍:
    0.047 ng/mL-3 ng/mL
  • 靈敏度:
    0.040 ng/mL
  • 反應時間:
    1-5h
  • 樣本體積:
    50-100ul
  • 檢測波長:
    450 nm
  • 研究領域:
    Immunology
  • 測定原理:
    quantitative
  • 測定方法:
    Sandwich
  • 精密度:

     

    Intra-assay Precision (Precision within an assay): CV%<8%

    Three samples of known concentration were tested twenty times on one plate to assess.

    Inter-assay Precision (Precision between assays): CV%<10%

    Three samples of known concentration were tested in twenty assays to assess.

     

  • 線性度:

     

    To assess the linearity of the assay, samples were spiked with high concentrations of pig TNFA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

     

  • 標準曲線:

     

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

     

  • 數據處理:
  • 貨期:
    3-5 working days

引用文獻

產品評價

平均分:
2.5分 - 2 個評價

樣品類型:細胞培養上清液

樣品信息:其他 豬

稀釋比:沒有稀釋

產品評價: 結果很滿意,也是對比了別家的試劑盒,最后發現還是華美的好用,我用的是細胞培養基上清,別家的試劑盒有干擾因素,華美的沒有,試了好幾家,也就這個牌子的好用。

By 高老師

樣品類型:血清

樣品信息:其他 豬

稀釋比:沒有稀釋

產品評價: 出于一直以來對華美產品的信任,這一次測定豬血清還是使用了華美的試劑盒,效果有些差異,可能和樣本有關,打算再測幾次。

By 楊老師

靶點詳情

  • 最新研究進展:
    TNF,全稱為腫瘤壞死因子(tumor necrosis factor),是一種由巨噬細胞、T細胞和其他免疫細胞分泌的細胞因子。最新的研究表明,TNF參與調節多種生理和病理過程,包括炎癥、免疫調節、細胞凋亡、細胞增殖和腫瘤發生等。目前,TNF在治療多種自身免疫性疾病和某些腫瘤中已得到廣泛應用,并持續吸引著研究者們的關注和研究。
  • 功能:
    Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation. Induces insulin resistance in adipocytes via inhibition of insulin-induced IRS1 tyrosine phosphorylation and insulin-induced glucose uptake. Induces GKAP42 protein degradation in adipocytes which is partially responsible for TNF-induced insulin resistance. Plays a role in angiogenesis by inducing VEGF production synergistically with IL1B and IL6.; The TNF intracellular domain (ICD) form induces IL12 production in dendritic cells.
  • 基因功能參考文獻:
    1. these findings provide the direct evidence that ADAM17 cleaves porcine TNFalpha, which represents a new view for identifying potential therapeutic targets in anti-porcine reproductive and respiratory syndrome virus therapy PMID: 26724939
    2. TMZ pretreatment effectively reduced the myocardial damage caused by CME via inhibiting the PDCD4/NF-kappaB/ TNF-alpha pathway in cardiomyocytes. PMID: 28683436
    3. TNF-alpha, secreted from activated Monocytes, mediates the downregulation of OTX2 and essential retinal pigment epithelium genes. PMID: 27660103
    4. TNF-alpha induced MMP-13 expression by condylar cells might be involved in the degradation of the juvenile condyle. PMID: 27618228
    5. TNF-alpha was able to promote theca interna cell proliferation. Our results suggest that TNF-alpha might play a role in hyperandrogenism, cortex thickness, and the increased ovary volume observed in polycystic ovaries. PMID: 27125399
    6. This study showed that the -791(C-->T) mutation of the TNF-alpha gene could be considered an important potential genetic marker of enterotoxigenic Escherichia coli F18 resistance. PMID: 27408333
    7. Both Nsp1beta and Nsp11 of porcine reproductive and respiratory syndrome virus were demonstrated to be responsible for the inhibitory effect on TNF-alpha production in pulmonary alveolar macrophages. PMID: 25708177
    8. Study presents evidence demonstrating a single species of exotoxin ApxI, derived from A. pleuropneumoniae serotype 10, induces the expression and production of proinflammatory cytokines IL-1beta, IL-8 and TNF-alpha in porcine alveolar macrophages. PMID: 21314908
    9. the role of miR-181a in adipocyte differentiation by regulation of TNF-alpha PMID: 24098322
    10. The NOS inhibitor, L-NMMA, significantly suppressed the combined effects of HT and CORM-2 on TNFalpha-triggered NFkappaBp65 phosphorylation as well as decreased cell viability. PMID: 24066302
    11. High-volume hemofiltration improves hemodynamics and heart dysfunction in septic shock pigs, which may be attributed to reduction of TNF-alpha in myocardium but not in circulation. PMID: 22994292
    12. Basal lipogenesis was not affected by tumor necrosis factor alpha (TNFalpha) treatment; however, insulin stimulated lipogenesis was reduced by TNFalpha. Interleukin 6 and TNFalpha gene expression were acutely (2-4 h) stimulated by exogenous TNFalpha treatment. PMID: 23090779
    13. In swine, IL-8, TNF-ALPHA, INOS AND MIP-1BETA were increased during mechanical ventilation in a time-related fashion. PMID: 23102097
    14. These data demonstrate that CRF triggers increases in intestinal paracellular permeability via mast cell dependent release of TNF-alpha and proteases. PMID: 22768175
    15. We identified critical amino acid residues in PRRSV Nsp1alpha and Nsp1beta that are important for TNF-alpha down-regulation and attenuation in vivo. PMID: 22699004
    16. Porcine reproductive and respiratory syndrome virus infection impaired TNF-alpha production by inhibiting ERK signaling. pathway. PMID: 22497732
    17. Data show that all five molecules, BNP, ICAM-1, TNF-alpha, VCAM-1 and IL-6, quickly and reliably signaled adverse interactions. PMID: 21471537
    18. These results suggest that trans-10, cis-12-conjugated linoleic acid can modulate TNF-alpha production and NF-kappa B expression by a PPARgamma-dependent pathway in porcine peripheral blood mononuclear cells. PMID: 21205430
    19. Retinal ischemia results in increased expression of TNF-alpha and its receptors (TNF-R1 and TNF-R2). PMID: 21152396
    20. increased plasma levels during recovery from cardiac arrest are associated with left ventricle dysfunction PMID: 19642909
    21. Viral non-structural protein 1 suppressed host TNF-alpha promoter by inhibiting NF-kappaB and Sp1 promoter binding activity. PMID: 20701940
    22. Exposure of porcine oocytes to an elevated TNF-alpha concentration clearly caused a reduction in their maturation from GV stage to MII stage and increased the proportion of oocytes with abnormal chromosome alignment and cytoskeleton structure PMID: 19324350
    23. Survival in the setting of severe, acute trauma/hemorrhagic shock appears to be associated with an immediate increase in serum TNF-alpha. PMID: 20027315
    24. These results suggest that TNF-alpha and IL-10, but not IL-6, are involved in the late reparatory phases of the experimental disk lesion. PMID: 19605905
    25. Repression of TNF-alpha associated with moderate hypothermia during experimental cardiac surgery is due to inhibition of the mitogen-activated protein kinase p38/activating protein-1 pathway and not to inhibition of NF-kappa B. PMID: 16606437
    26. Stenting of coronary arteries was associated with an eight-fold enhancement in TNFalpha expression in injured arterial tissue. PMID: 16857205
    27. The effect of EGF on pro-inflammatory cytokines, tumor necrosis factor-alpha (TNF-alpha) and cyclooxygenase-2 (COX-2), levels during wound healing in swine is reported. PMID: 17098266
    28. In coronary microembolization, TNF-alpha is responsible for both progressive contractile dysfunction and delayed protection against infarction. PMID: 17170366
    29. The data indicate that the development of clinical symptoms of coliform mastitis in the sow is associated with a locally increased proinflammatory cytokine production in response to intramammary Escherichia coli infection. PMID: 17903420
    30. the ERK/GEF-H1/Rho/Rho kinase/phospho-MLC pathway is the mechanism mediating TNF-alpha-induced elevation of tubular epithelial permeability, which in turn might contribute to kidney injury PMID: 19261619
    31. Data show that cysteine significantly reduced the expression of pro-inflammatory cytokines, including TNF-alpha, IL-6, IL-12p40, IL-1beta, and resulted in increased expression of the apoptosis initiator caspase-8 and bcl2L1. PMID: 19520150
    32. TNF-alpha inhibits adipogenesis through stabilization of beta-catenin protein in porcine preadipocytes. PMID: 19558791

    顯示更多

    收起更多

  • 亞細胞定位:
    Cell membrane; Single-pass type II membrane protein.; [Tumor necrosis factor, membrane form]: Membrane; Single-pass type II membrane protein.; [Tumor necrosis factor, soluble form]: Secreted.; [C-domain 1]: Secreted.; [C-domain 2]: Secreted.
  • 蛋白家族:
    Tumor necrosis factor family
  • 數據庫鏈接:

    KEGG: ssc:397086

    STRING: 9823.ENSSSCP00000001491

    UniGene: Ssc.100



主站蜘蛛池模板: 国产sm重味一区二区三区| 国产精品午夜福利麻豆| 国内午夜国产精品小视频| 国产清纯美女遭强到高潮| 97av麻豆蜜桃一区二区| 久久婷婷成人综合色综合| 日韩经典午夜福利发布| 亚洲精品国产av天美传媒| 久久久久青草线综合超碰| 日产精品卡2卡三卡乱码网站| 久久综合av免费观看| 中文字幕乱码亚洲无线三区| 国产成a人亚洲精品无码樱花 | 日本三级理论久久人妻电影| 99久久无色码中文字幕人妻 | 欧美疯狂做受xxxxx高潮| 成人乱人伦视频在线观看| 久久精品国产一区二区免费| 国产性生交xxxxx无码| 久久精品中文字幕无码绿巨人| 国产精品亚洲一区二区三区| 狠狠久久久久综合成人影院 | 久久精品a亚洲国产v高清不卡| 手机看片国产av无码| 久久人人97超碰a片精品| 日本一区二区三区免费播放| 在线观看特色大片免费网站| 日本熟妇色xxxxxhd| 人妻少妇伦在线麻豆m电影| 最近最新中文字幕高清免费| 国产午夜福利在线观看视频| 鲁丝片一区二区三区毛片| 男女午夜猛烈啪啦啦视频| 综合图区亚洲欧美另类图片| 国产精品9999久久久久仙踪林| 免费无码a片一区二三区| 免费啪视频在线观看视频日本 | 色婷婷欧美在线播放内射| 一本久道高清无码视频| 纯肉无遮挡无码日本动漫| 女高中生自慰污污网站|