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Pig Renin ELISA Kit

  • 中文名稱:
    豬腎素(Renin)酶聯(lián)免疫試劑盒
  • 貨號:
    CSB-E17914p
  • 規(guī)格:
    96T/48T
  • 價格:
    ¥3800/¥2500
  • 其他:

產(chǎn)品詳情

  • 產(chǎn)品描述:
    豬腎素(Renin)酶聯(lián)免疫試劑盒(CSB-E17914p)為雙抗夾心法ELISA試劑盒,定量檢測血清、血漿、組織勻漿樣本中的REN含量。試劑盒檢測范圍為62.5 pg/mL-4000 pg/mL,該產(chǎn)品適用于科研場景中與Renin相關的動物模型研究,如高血壓機制探索、腎臟病理分析或藥物干預實驗,為評估豬心血管及腎臟系統(tǒng)的生理病理變化提供可靠工具,滿足基礎研究中對目標蛋白精準定量的需求。本品僅用于科研,不用于臨床診斷,產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名:
    FLJ10761, HNFJ2, angiotensin-forming enzyme|angiotensinogenase|renin precursor, renal
  • 縮寫:
  • 種屬:
    Sus scrofa (Pig)
  • 樣本類型:
    serum, plasma, tissue homogenates
  • 檢測范圍:
    62.5 pg/mL-4000 pg/mL
  • 靈敏度:
    15.6 pg/mL
  • 反應時間:
    1-5h
  • 樣本體積:
    50-100ul
  • 檢測波長:
    450 nm
  • 研究領域:
    Cardiovascular
  • 測定原理:
    quantitative
  • 測定方法:
    Sandwich
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<8%        
    Three samples of known concentration were tested twenty times on one plate to assess.    
    Inter-assay Precision (Precision between assays): CV%<10%        
    Three samples of known concentration were tested in twenty assays to assess.      
                   
  • 線性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of pig renin in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.  
      Sample Serum(n=4)    
    1:1 Average % 95    
    Range % 85-103    
    1:2 Average % 92    
    Range % 85-102    
    1:4 Average % 93    
    Range % 83-99    
    1:8 Average % 95    
    Range % 90-110    
  • 回收率:
    The recovery of pig renin spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.  
     
    Sample Type Average % Recovery Range    
    Serum (n=5) 95 82-102    
    EDTA plasma (n=4) 94 86-100    
                   
                   
  • 標準曲線:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.  
     
    pg/ml OD1 OD2 Average Corrected    
    4000 1.973 1.997 1.985 1.867    
    2000 1.635 1.643 1.639 1.521    
    1000 1.208 1.197 1.203 1.085    
    500 0.805 0.796 0.801 0.683    
    250 0.549 0.544 0.547 0.429    
    125 0.361 0.356 0.359 0.241    
    62.5 0.255 0.259 0.257 0.139    
    0 0.116 0.119 0.118      
  • 數(shù)據(jù)處理:
  • 貨期:
    3-5 working days

產(chǎn)品評價

相關問答

 常見問題解答
Q:

I have a customer who interested in both Human renin, REN ELISA Kit CSB-E08701h & Pig Renin ELISA Kit CSB-E17914p
They would like to know if they can use whole blood in these two kits?

A:
Thanks for your inquiry!
Renin, angiotensin constitute renin-angiotensin system (RAS) or renin, angiotensin, aldosterone constitute renin-angiotensin-aldosterone system (RAAS) together to participate in body fluid balance.
Both kits are suitable to serum and plasma samples.They are for quantitative measurement of renin. To test whole blood is not advised.
Pls let me know if you have any further questions. Thank you.


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