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Pig Pancreatic triacylglycerol lipase(PNLIP) ELISA kit

  • 中文名稱:
    豬胰腺三酰基甘油脂肪酶(PNLIP)酶聯免疫試劑盒
  • 貨號:
    CSB-EL018262PI
  • 規格:
    96T/48T
  • 價格:
    ¥4200/¥3000
  • 其他:

產品詳情

  • 產品描述:
    豬胰腺三酰基甘油脂肪酶(PNLIP)酶聯免疫試劑盒(CSB-EL018262PI)為雙抗夾心法ELISA試劑盒,定量檢測血清、血漿、組織勻漿、細胞裂解物樣本中的PNLIP含量。PNLIP即胰腺脂肪酶,是消化脂肪的關鍵酶。其背景在于它對膳食脂肪的消化吸收至關重要。研究機制方面,聚焦于其催化脂肪水解的過程,了解其活性調節機制,以此為靶點開發調節脂肪消化的藥物,為肥胖、高脂血癥等治療提供思路。試劑盒檢測范圍為0.625 mU/mL-40 mU/mL,該產品適用于動物模型研究、營養學實驗中胰腺功能評估、消化酶活性動態監測,以及藥物或化合物對脂質代謝影響的體外分析。實驗操作兼容多種生物樣本類型,為探索胰腺生理機制、代謝性疾病發病機理等基礎研究提供可靠工具。本品僅用于科研,不用于臨床診斷,產品具體參數及操作步驟詳見產品說明書。
  • 別名:
    PNLIPPancreatic triacylglycerol lipase ELISA Kit; PL ELISA Kit; PTL ELISA Kit; Pancreatic lipase ELISA Kit; EC 3.1.1.3 ELISA Kit
  • 縮寫:
  • Uniprot No.:
  • 種屬:
    Sus scrofa (Pig)
  • 樣本類型:
    serum, plasma, tissue homogenates, cell lysates
  • 檢測范圍:
    0.625 mU/mL-40 mU/mL
  • 靈敏度:
    0.156 mU/mL
  • 反應時間:
    1-5h
  • 樣本體積:
    50-100ul
  • 檢測波長:
    450 nm
  • 研究領域:
    Metabolism
  • 測定原理:
    quantitative
  • 測定方法:
    Sandwich
  • 精密度:

    Intra-assay Precision (Precision within an assay): CV%<8%

    Three samples of known concentration were tested twenty times on one plate to assess.

    Inter-assay Precision (Precision between assays): CV%<10%

    Three samples of known concentration were tested in twenty assays to assess.

  • 線性度:

    To assess the linearity of the assay, samples were spiked with high concentrations of pig PNLIP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

     

    Sample

    Serum(n=4)

    1:1

    Average %

    94

    Range %

    86-100

    1:2

    Average %

    95

    Range %

    82-107

    1:4

    Average %

    92

    Range %

    81-103

    1:8

    Average %

    96

    Range %

    88-103

  • 回收率:

    The recovery of pig PNLIP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample Type

    Average % Recovery

    Range

    Serum (n=5)

    95

    87-106

    EDTA plasma (n=4)

    97

    89-112

  • 標準曲線:

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

    mU/ml

    OD1

    OD2

    Average

    Corrected

    40

    2.052

    2.137

    2.095

    2.007

    20

    1.639

    1.658

    1.649

    1.561

    10

    1.110

    1.134

    1.122

    1.034

    5

    0.753

    0.711

    0.732

    0.644

    2.5

    0.405

    0.392

    0.399

    0.311

    1.25

    0.312

    0.303

    0.308

    0.220

    0.625

    0.189

    0.181

    0.185

    0.097

    0

    0.086

    0.090

    0.088

     

  • 數據處理:
  • 貨期:
    3-5 working days

產品評價

靶點詳情

  • 功能:
    Plays an important role in fat metabolism. It preferentially splits the esters of long-chain fatty acids at positions 1 and 3, producing mainly 2-monoacylglycerol and free fatty acids, and shows considerably higher activity against insoluble emulsified substrates than against soluble ones.
  • 亞細胞定位:
    Secreted.
  • 蛋白家族:
    AB hydrolase superfamily, Lipase family
  • 數據庫鏈接:


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