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Pig Neuron-specific enolase,NSE ELISA Kit

  • 中文名稱:
    豬神經(jīng)特異性烯醇化酶(NSE)酶聯(lián)免疫試劑盒
  • 貨號:
    CSB-E14065p
  • 規(guī)格:
    96T/48T
  • 價格:
    ¥4200/¥3000
  • 其他:

產(chǎn)品詳情

  • 產(chǎn)品描述:
    豬神經(jīng)特異性烯醇化酶(NSE)酶聯(lián)免疫試劑盒(CSB-E14065p)為雙抗夾心法ELISA試劑盒,定量檢測血清、血漿、組織勻漿樣本中的NSE含量。試劑盒檢測范圍為0.78 ng/mL-50 ng/mL,適用于腦組織、外周神經(jīng)等樣本的神經(jīng)功能評估,兼容常規(guī)血清/血漿樣本(需離心去除沉淀后分裝凍存),可滿足神經(jīng)退行性疾病模型構(gòu)建、神經(jīng)損傷修復(fù)研究以及神經(jīng)內(nèi)分泌相關(guān)疾病機制探索等科研需求,為動物實驗中的病理機制解析和藥物干預(yù)效果觀察提供可靠定量工具。本品僅用于科研,不用于臨床診斷,產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 縮寫:
    NSE
  • 種屬:
    Sus scrofa (Pig)
  • 樣本類型:
    serum, plasma, tissue homogenates
  • 檢測范圍:
    0.78 ng/mL-50 ng/mL
  • 靈敏度:
    0.195 ng/mL
  • 反應(yīng)時間:
    1-5h
  • 樣本體積:
    50-100ul
  • 檢測波長:
    450 nm
  • 研究領(lǐng)域:
    Cancer
  • 測定原理:
    quantitative
  • 測定方法:
    Sandwich
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of pig NSE in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:1Average %91
    Range %86-102
    1:2Average %90
    Range %85-99
    1:4Average %95
    Range %87-98
    1:8Average %94
    Range %86-104
  • 回收率:
    The recovery of pig NSE spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9584-102
    EDTA plasma (n=4)9483-99
  • 標準曲線:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/mlOD1OD2AverageCorrected
    502.754 2.682 2.718 2.614
    252.017 1.997 2.007 1.903
    12.51.330 1.362 1.346 1.242
    6.250.944 0.957 0.951 0.847
    3.120.653 0.641 0.647 0.543
    1.560.378 0.369 0.374 0.270
    0.780.240 0.251 0.246 0.142
    00.102 0.105 0.104
  • 數(shù)據(jù)處理:
  • 貨期:
    3-5 working days

產(chǎn)品評價



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