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Pig Interleukin 22(IL-22) ELISA Kit

  • 中文名稱:
    豬白介素22(IL-22)酶聯免疫試劑盒
  • 貨號:
    CSB-E15847p
  • 規格:
    96T/48T
  • 價格:
    ¥3800/¥2500
  • 其他:

產品詳情

  • 產品描述:
    豬白介素22(IL-22)酶聯免疫試劑盒(CSB-E15847p)為雙抗夾心法ELISA試劑盒,定量檢測血清、血漿、組織勻漿樣本中的IL-22含量。IL-22是重要靶點。它是一種細胞因子,參與免疫調節。其背景在于在多種疾病如炎癥性腸病、皮膚病等中表達異常。研究機制方面,主要通過與受體結合,激活下游信號通路,影響細胞增殖、分化及炎癥反應,為相關疾病治療提供方向。試劑盒檢測范圍為78 pg/mL-5000 pg/mL,適用于動物免疫調控研究、病原感染后細胞因子表達分析、腸道屏障功能評估等科研方向,為探索豬類疾病模型、疫苗效果評價及營養干預研究提供可靠工具。本品僅用于科研,不用于臨床診斷,產品具體參數及操作步驟詳見產品說明書。
  • 縮寫:
    IL-22
  • Uniprot No.:
  • 種屬:
    Sus scrofa (Pig)
  • 樣本類型:
    serum, plasma, tissue homogenates
  • 檢測范圍:
    78 pg/mL-5000 pg/mL
  • 靈敏度:
    19.5 pg/mL
  • 反應時間:
    1-5h
  • 樣本體積:
    50-100ul
  • 檢測波長:
    450 nm
  • 研究領域:
    Immunology
  • 測定原理:
    quantitative
  • 測定方法:
    Sandwich
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of pig IL-22 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)
    1:1 Average % 99
    Range % 94-104
    1:2 Average % 96
    Range % 89-100
    1:4 Average % 95
    Range % 89-98
    1:8 Average % 103
    Range % 95-107
  • 回收率:
    The recovery of pig IL-22 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range
    Serum (n=5) 99 91-103
    EDTA plasma (n=4) 101 95-107
  • 標準曲線:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average Corrected
    5000 2.162 2.085 2.124 1.948
    2500 1.875 1.811 1.843 1.667
    1250 1.469 1.383 1.426 1.250
    625 0.787 0.764 0.776 0.600
    312 0.484 0.472 0.478 0.302
    156 0.315 0.304 0.310 0.134
    78 0.231 0.228 0.230 0.054
    0 0.178 0.174 0.176  
  • 數據處理:
  • 貨期:
    3-5 working days

產品評價



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