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Pig Interleukin 12,IL-12/P40 ELISA KIT

  • 中文名稱:
    豬白介素12(IL-12/P40)酶聯免疫試劑盒
  • 貨號:
    CSB-E11341p
  • 規格:
    96T/48T
  • 價格:
    ¥4200/¥3000
  • 其他:

產品詳情

  • 產品描述:
    豬白介素12(IL-12/P40)酶聯免疫試劑盒(CSB-E11341p)為雙抗夾心法ELISA試劑盒,定量檢測血清、血漿、組織培養上清液樣本中的IL12B含量。IL12B是一種編碼白細胞介素12亞單位的基因,參與T細胞和自然殺傷細胞的免疫反應。其研究機制涉及IL12B與IL12A結合形成異源二聚體,激活Jak-STAT信號通路,調節免疫應答和炎癥反應。試劑盒檢測范圍為15.6 pg/mL-1000 pg/mL,其水平檢測可為感染性疾病、自身免疫研究及疫苗開發等科研領域提供重要參考;適用于評估實驗動物模型中的免疫應答強度、細胞因子網絡調控機制研究,或體外藥物篩選過程中炎癥介質的動態監測;為科研人員探索豬源IL - 12/P40在免疫病理學中的功能提供可靠工具。本品僅用于科研,不用于臨床診斷,產品具體參數及操作步驟詳見產品說明書。
  • 別名:
    IL12BInterleukin-12 subunit beta ELISA Kit; IL-12B ELISA Kit; Cytotoxic lymphocyte maturation factor 40 kDa subunit ELISA Kit; CLMF p40 ELISA Kit; IL-12 subunit p40 ELISA Kit
  • 縮寫:
  • Uniprot No.:
  • 種屬:
    Sus scrofa (Pig)
  • 樣本類型:
    serum, plasma, cell culture supernates
  • 檢測范圍:
    15.6 pg/mL-1000 pg/mL
  • 靈敏度:
    3.9 pg/mL
  • 反應時間:
    1-5h
  • 樣本體積:
    50-100ul
  • 檢測波長:
    450 nm
  • 研究領域:
    Immunology
  • 測定原理:
    quantitative
  • 測定方法:
    Sandwich
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of pig IL-12/P40 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
     SampleSerum(n=4)
    1:1Average %88
    Range %80-99
    1:2Average %95
    Range %90-104
    1:4Average %98
    Range %92-110
    1:8Average %95
    Range %85-105
  • 回收率:
    The recovery of pig IL-12/P40 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9585-104
    EDTA plasma (n=4)9890-112
  • 標準曲線:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    10002.028 2.157 2.093 1.884
    5001.562 1.612 1.587 1.378
    2500.980 1.034 1.007 0.798
    1250.570 0.603 0.587 0.378
    62.50.352 0.362 0.357 0.148
    31.250.303 0.311 0.307 0.098
    15.60.282 0.268 0.275 0.066
    00.214 0.204 0.209  
  • 數據處理:
  • 貨期:
    3-5 working days

產品評價

靶點詳情

  • 功能:
    Cytokine that can act as a growth factor for activated T and NK cells, enhance the lytic activity of NK/lymphokine-activated killer cells, and stimulate the production of IFN-gamma by resting PBMC.; Associates with IL23A to form the IL-23 interleukin, a heterodimeric cytokine which functions in innate and adaptive immunity. IL-23 may constitute with IL-17 an acute response to infection in peripheral tissues. IL-23 binds to a heterodimeric receptor complex composed of IL12RB1 and IL23R, activates the Jak-Stat signaling cascade, stimulates memory rather than naive T-cells and promotes production of proinflammatory cytokines. IL-23 induces autoimmune inflammation and thus may be responsible for autoimmune inflammatory diseases and may be important for tumorigenesis.
  • 基因功能參考文獻:
    1. Data show that cysteine significantly reduced the expression of pro-inflammatory cytokines, including TNF-alpha, IL-6, IL-12p40, IL-1beta, and resulted in increased expression of the apoptosis initiator caspase-8 and bcl2L1. PMID: 19520150
  • 亞細胞定位:
    Secreted.
  • 蛋白家族:
    Type I cytokine receptor family, Type 3 subfamily
  • 數據庫鏈接:

    KEGG: ssc:397076

    STRING: 9823.ENSSSCP00000018057

    UniGene: Ssc.71



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