在线日韩日本国产亚洲丨少妇伦子伦情品无吗丨欧美性猛交xxxx免费看蜜桃丨精品人妻系列无码一区二区三区丨亚洲精品无码不卡在线播放

Your Good Partner in Biology Research

  1. 首頁
  2. 試劑盒
  3. 酶聯免疫試劑盒
  4. Pig Interferon γ,IFN-γ ELISA Kit

Pig Interferon γ,IFN-γ ELISA Kit

  • 中文名稱：
    豬γ干擾素(IFN-γ)酶聯免疫試劑盒
  • 貨號：
    CSB-E06794p
  • 規格：
    96T/48T
  • 價格：
    ￥4200/￥3000
  • 其他：

產品詳情

  • 產品描述：
    豬γ干擾素(IFN-γ)酶聯免疫試劑盒（CSB-E06794p）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的IFNG含量。IFNG即干擾素-γ，是一類具有多種生物學活性的細胞因子。它在免疫調節、抗病毒和抗腫瘤等方面發揮關鍵作用。研究機制上，IFNG能激活免疫細胞，增強其殺傷功能，還可調節多種免疫相關基因表達，是免疫領域的重要研究靶點。試劑盒檢測范圍為15.6 pg/mL-1000 pg/mL，適用于豬類動物模型中感染性疾病研究、疫苗免疫效果評估、免疫調節機制探索等科研領域，為評估宿主免疫狀態及炎癥反應提供可靠工具本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
  • 別名：
    IFNGInterferon gamma ELISA Kit; IFN-gamma ELISA Kit
  • 縮寫：
  • Uniprot No.：
  • 種屬：
    Sus scrofa (Pig)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測范圍：
    15.6 pg/mL-1000 pg/mL
  • 靈敏度：
    3.9 pg/mL
  • 反應時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領域：
    Immunology
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of pig IFN-γ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:1 Average % 94  
    Range % 90-99  
    1:2 Average % 92  
    Range % 85-99  
    1:4 Average % 95  
    Range % 91-102  
    1:8 Average % 95  
    Range % 90-100  
  • 回收率：
    The recovery of pig IFN-γ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 95 86-102  
    EDTA plasma (n=4) 96 89-100  
                 
                 
  • 標準曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average Corrected  
    1000 2.692 2.647 2.670 2.581  
    500 2.404 2.465 2.435 2.346  
    250 1.974 1.989 1.982 1.893  
    125 1.523 1.541 1.532 1.443  
    62.5 0.945 0.942 0.944 0.855  
    31.2 0.613 0.635 0.624 0.535  
    15.6 0.337 0.324 0.331 0.242  
    0 0.090 0.087 0.089 　  
  • 數據處理：
  • 貨期：
    3-5 working days

引用文獻

產品評價

靶點詳情

  • 功能：
    Type II interferon produced by immune cells such as T-cells and NK cells that plays crucial roles in antimicrobial, antiviral, and antitumor responses by activating effector immune cells and enhancing antigen presentation. Primarily signals through the JAK-STAT pathway after interaction with its receptor IFNGR1 to affect gene regulation. Upon IFNG binding, IFNGR1 intracellular domain opens out to allow association of downstream signaling components JAK2, JAK1 and STAT1, leading to STAT1 activation, nuclear translocation and transcription of IFNG-regulated genes. Many of the induced genes are transcription factors such as IRF1 that are able to further drive regulation of a next wave of transcription. Plays a role in class I antigen presentation pathway by inducing a replacement of catalytic proteasome subunits with immunoproteasome subunits. In turn, increases the quantity, quality, and repertoire of peptides for class I MHC loading. Increases the efficiency of peptide generation also by inducing the expression of activator PA28 that associates with the proteasome and alters its proteolytic cleavage preference. Up-regulates as well MHC II complexes on the cell surface by promoting expression of several key molecules such as cathepsins B/CTSB, H/CTSH, and L/CTSL. Participates in the regulation of hematopoietic stem cells during development and under homeostatic conditions by affecting their development, quiescence, and differentiation.
  • 基因功能參考文獻：
    1. The regulatory effect of IFN-gamma on CYP3A29 expression is mediated via PXR. PMID: 25413352
    2. Mapping of QTL for mycoplasma and tetanus antibodies and IFNgamma. PMID: 20567833
    3. Data show taht the expression of PoIFN-gamma in insect cells was confirmed by Western Blot, indirect immunofluorescence assay and indirect ELISA. PMID: 17577980
    4. IFNgamma production from the frozen peripheral blood mononuclear cells was significantly higher than that from the fresh ones. PMID: 19766617
    5. Translational control of IL-18 expression by its 5'-UTR limits production of IL-18, resulting in restricted expression of mRNA and protein IFN-gamma in this model of crescentic glomerulonephritis(GN). Might amplify CD8+-mediated macrophage-dependent GN. PMID: 12787406
    6. selective changes in immature crypt cells induced by IFN-gamma bound to extracellular matrix could contribute to inappropriate responsiveness to commensal bacteria in inflammatory bowel diseases PMID: 15894119
    7. A functional single nucleotide polymorphism is reported in the coding region of IFN-gamma cDNA that markedly reduces antiviral activity of the IFN-gamma protein. PMID: 17416419
    8. The expression of IFN-gamma in recombiannt Lactococcus lactis as a tool for investigating downregulation of allergic predisposition of pigs to experimental food allergy is reported. PMID: 18164876

    顯示更多

    收起更多

  • 亞細胞定位：
    Secreted.
  • 蛋白家族：
    Type II (or gamma) interferon family
  • 組織特異性：
    Released primarily from activated T lymphocytes.
  • 數據庫鏈接：


主站蜘蛛池模板： 老司机午夜免费精品视频| 亚洲大成色www永久网站动图| 狠狠cao2020高清视频| 一本久久伊人热热精品中文字幕| 国产在线精品欧美日韩电影| 久久伊人av综合影院| 久久久久久久久无码精品亚洲日韩| 男人和女人高潮免费网站| 狂野av人人澡人人添| 侵犯强奷高清无码| 国产成人av在线一区二区三区| 国产草草影院ccyycom| 久久青草费线频观看| 免费夜色污私人影院在线观看| 忘忧草日本在线播放www| 亚洲a∨精品一区二区三区| 亚洲a无码综合a国产av中文| 亚洲a成人片在线观看 | 成在人线av无码免费| 亚洲熟妇无码av另类vr影视| 亚洲国产精品av在线播放| 色八区人妻在线视频免费| 久久国产精品无码一区二区三区| 九色porny丨国产首页注册| 亚洲日韩欧美一区久久久久我| 中文字幕+乱码+中文字幕一区| 精品无码一区二区三区的天堂| 亚洲精品乱码久久久久久蜜桃图片| 四虎影视国产精品久久| 国产又粗又猛又爽的视频a片| 夜夜爱夜鲁夜鲁很鲁| 亚洲午夜国产精品无码老牛影视| 亚洲国产午夜精品理论片| 国产精品高潮呻吟av久久| 日韩吃奶摸下aa片免费观看| 欧美黑人添添高潮a片www| 男女真人国产牲交a做片野外| 久久精品国产只有精品96| 亚洲人成人网站在线观看| 亚洲精品成人无码中文毛片不卡| 国产精品国产三级国av麻豆|