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  2. 試劑盒
  3. 酶聯(lián)免疫試劑盒
  4. Pig Immunoglobulin A,IgA ELISA Kit
  • 中文名稱(chēng)：
    豬免疫球蛋白A(IgA)酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-E13234p
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥4200/￥3000
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    豬免疫球蛋白A(IgA)酶聯(lián)免疫試劑盒（CSB-E13234p）為競(jìng)爭(zhēng)法ELISA試劑盒，定量檢測(cè)血清、血漿樣本中的IgA含量。IgA是免疫球蛋白A，是機(jī)體黏膜免疫的主要抗體，在抵御病原體入侵上發(fā)揮重要作用。研究機(jī)制上，主要聚焦于其與各類(lèi)疾病的關(guān)聯(lián)，如感染性疾病、自身免疫病等，通過(guò)研究其產(chǎn)生、功能及調(diào)節(jié)機(jī)制，來(lái)探尋疾病防治新策略。試劑盒檢測(cè)范圍為0.146 μg/mL-37.5 μg/mL，本試劑盒適用于科研領(lǐng)域?qū)ωi黏膜免疫功能的評(píng)估研究。該產(chǎn)品適用于畜牧科研中疫苗效力評(píng)估、疾病模型建立時(shí)的免疫應(yīng)答監(jiān)測(cè)，或規(guī)模化養(yǎng)殖中通過(guò)定期檢測(cè) IgA 水平分析豬群健康狀態(tài)。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書(shū)。
  • 縮寫(xiě)：
    IgA
  • 種屬：
    Sus scrofa (Pig)
  • 樣本類(lèi)型：
    serum, plasma
  • 檢測(cè)范圍：
    0.146 μg/mL-37.5 μg/mL
  • 靈敏度：
    0.146 μg/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測(cè)波長(zhǎng)：
    450 nm
  • 研究領(lǐng)域：
    Immunology
  • 測(cè)定原理：
    quantitative
  • 測(cè)定方法：
    Competitive
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of pig IgA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:1000 Average % 92  
    Range % 88-94  
    1:2000 Average % 93  
    Range % 90-98  
    1:4000 Average % 88  
    Range % 85-94  
    1:8000 Average % 95  
    Range % 91-100  
  • 回收率：
    The recovery of pig IgA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 94 89-97  
    EDTA plasma (n=4) 103 98-106  
                 
                 
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    μg/ml OD1 OD2 Average    
    37.5 0.199 0.209 0.204    
    9.375 0.445 0.420 0.433    
    2.345 0.924 0.955 0.940    
    0.586 1.413 1.447 1.430    
    0.146 2.101 2.114 2.108    
    0 2.547 2.503 2.525    
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

引用文獻(xiàn)

產(chǎn)品評(píng)價(jià)

平均分:
5.0分 - 1 個(gè)評(píng)價(jià)

樣品類(lèi)型：血清

樣品信息：其他 豬

稀釋比：1:100

產(chǎn)品評(píng)價(jià)： 標(biāo)曲準(zhǔn)確，樣本數(shù)值在標(biāo)曲范圍之內(nèi)，符合預(yù)期結(jié)果。

By 謝老師

相關(guān)問(wèn)答

■ 常見(jiàn)問(wèn)題解答
Q:

Hi. I would like to know if this kit is used feces samples from pig. If so, I would like knowing the cost. Also I would like to know if you have avilable the kit with reagents for plate preparation and its respective cost, as well. Thanks much.

A:
Thanks for your inquiry.
For IgA, there are serotype and secretory type. If you want to test feces samples, we recommend you choose Pig secretory immunoglobulin A,SIgA ELISA KitCSB-E12063p. No matter you use CSB-E12063p or CSB-E13234p, we suggest you do a pretest first.
All the components in the kit are well prepared, so you don't need to prepare the reagents by yourself. Sorry we can not provide reagents for plate preparation.Pls let me know if you have any further questions. Thank you.