Pig Creatine kinase M-type(CKM) ELISA kit

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產品詳情

產品描述：

豬M型肌酸激酶(CKM)酶聯免疫試劑盒（CSB-EL005459PI）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的CKM含量。豬M型肌酸激酶（CKM）是一種肌肉特異性酶，主要存在于骨骼肌細胞中，參與能量代謝。其研究機制涉及基因表達調控，特別是其啟動子區域，通過特異性啟動下游基因表達，影響肌肉生長和品質。研究旨在通過基因工程技術提高豬肉品質和生長速度。試劑盒檢測范圍為0.156 mU/mL-10 mU/mL，在科研領域，本產品適用于探究豬肌肉生理病理機制、運動代謝調控、肌肉相關疾病模型建立等研究，也可用于評估營養干預或藥物處理對肌肉能量代謝的影響。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

CKMCreatine kinase M-type ELISA Kit; EC 2.7.3.2 ELISA Kit; Creatine kinase M chain ELISA Kit; Creatine phosphokinase M-type ELISA Kit; CPK-M ELISA Kit; M-CK ELISA Kit
縮寫：

CKM
Uniprot No.：

Q5XLD3
種屬：

Sus scrofa (Pig)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

0.156 mU/mL-10 mU/mL
靈敏度：

0.039 mU/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Metabolism
測定原理：

quantitative
測定方法：

Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of pig CKM in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:5	Average %	103
	Range %	92-107
1:10	Average %	98
	Range %	91-102
1:20	Average %	93
	Range %	84-97
1:40	Average %	99
	Range %	90-103

回收率：

The recovery of pig CKM spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	100	88-103
EDTA plasma (n=4)	92	84-96

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

功能：

Reversibly catalyzes the transfer of phosphate between ATP and various phosphogens (e.g. creatine phosphate). Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa.
基因功能參考文獻：
1. this study examined the heterogeneity of creatine kinase (CK) forward flux rate of hearts with postinfarction left ventricular (LV) remodeling. PMID: 27606901
蛋白家族：

ATP:guanido phosphotransferase family
數據庫鏈接：

KEGG: ssc:397264

UniGene: Ssc.415