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  4. Pig Brain-derived neurotrophic factor(BDNF) ELISA kit

Pig Brain-derived neurotrophic factor(BDNF) ELISA kit

  • 中文名稱：
    豬腦源性神經(jīng)營養(yǎng)因子(BDNF)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-EL002655PI
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥4200/￥3000
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    豬腦源性神經(jīng)營養(yǎng)因子(BDNF)酶聯(lián)免疫試劑盒（CSB-EL002655PI）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的BDNF含量。BDNF即腦源性神經(jīng)營養(yǎng)因子，在神經(jīng)系統(tǒng)發(fā)育和維持中扮演關(guān)鍵角色。研究顯示，它能促進(jìn)神經(jīng)元存活、生長和分化，增強(qiáng)突觸可塑性。其機(jī)制是與特定受體結(jié)合，激活細(xì)胞內(nèi)信號通路，進(jìn)而調(diào)控基因表達(dá)，影響神經(jīng)系統(tǒng)功能。試劑盒檢測范圍為9.375 ng/mL-600 ng/mL，豬模型研究中神經(jīng)退行性疾病機(jī)制探索、藥物干預(yù)效果評估或應(yīng)激狀態(tài)下神經(jīng)營養(yǎng)因子動態(tài)變化分析等科研場景，以及神經(jīng)科學(xué)、畜牧獸醫(yī)及比較醫(yī)學(xué)領(lǐng)域的實驗研究本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    BDNFBrain-derived neurotrophic factor ELISA kit; BDNF) [Cleaved into: BDNF precursor form ELISA kit; ProBDNF)] ELISA kit
  • 縮寫：
  • Uniprot No.：
  • 種屬：
    Sus scrofa (Pig)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測范圍：
    9.375 ng/mL-600 ng/mL
  • 靈敏度：
    2.35 ng/mL
  • 反應(yīng)時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Cardiovascular
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of pig BDNF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)
    1:1 Average % 96
    Range % 91-102
    1:2 Average % 102
    Range % 97-107
    1:4 Average % 109
    Range % 105-113
    1:8 Average % 103
    Range % 96-107
  • 回收率：
    The recovery of pig BDNF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range
    Serum (n=5) 102 98-105
    EDTA plasma (n=4) 86 93-92
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average Corrected
    600 2.134 2.081 2.108 2.016
    300 1.537 1.623 1.580 1.488
    150 1.167 1.123 1.145 1.053
    75 0.769 0.774 0.772 0.680
    37.5 0.513 0.503 0.508 0.416
    18.75 0.353 0.344 0.349 0.257
    9.375 0.227 0.221 0.224 0.132
    0 0.091 0.093 0.092 　
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評價

靶點詳情

  • 功能：
    Important signaling molecule that activates signaling cascades downstream of NTRK2. During development, promotes the survival and differentiation of selected neuronal populations of the peripheral and central nervous systems. Participates in axonal growth, pathfinding and in the modulation of dendritic growth and morphology. Major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS. The versatility of BDNF is emphasized by its contribution to a range of adaptive neuronal responses including long-term potentiation (LTP), long-term depression (LTD), certain forms of short-term synaptic plasticity, as well as homeostatic regulation of intrinsic neuronal excitability.; Important signaling molecule that activates signaling cascades downstream of NTRK2. Activates signaling cascades via the heterodimeric receptor formed by NGFR and SORCS2. Signaling via NGFR and SORCS2 plays a role in synaptic plasticity and long-term depression (LTD). Binding to NGFR and SORCS2 promotes neuronal apoptosis. Promotes neuronal growth cone collapse.
  • 基因功能參考文獻(xiàn)：
    1. measurement of brain derived neurotrophic factor in serum brings a practical approach to study the effects of environmental enrichment on neurobiological changes in domestic animals PMID: 30007130
    2. Lf upregulated several canonical signaling pathways associated with neurodevelopment and cognition and influenced ~10 genes involved in the brain-derived neurotrophin factor (BDNF) signaling pathway. PMID: 25146846
    3. Taken together, these data indicate that recurrent tethering stress in sows over 4.5 years results in a loss of neurotrophic support by BDNF, mediated by an overactive neuroendocrine system. PMID: 22584108
    4. FiO2 used for resuscitation affects matrix metalloproteinases MMP-9 and MMP-2, caspase-3 and BDNF PMID: 21151608
  • 亞細(xì)胞定位：
    Secreted.; [BDNF precursor form]: Secreted.
  • 蛋白家族：
    NGF-beta family
  • 組織特異性：
    Brain and central nervous system.
  • 數(shù)據(jù)庫鏈接：


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