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Mouse von Willebrand Factor,vWF ELISA Kit

  • 中文名稱:
    小鼠血管性血友病因子/瑞斯托霉素輔因子(vWF)酶聯(lián)免疫試劑盒
  • 貨號:
    CSB-E08439m
  • 規(guī)格:
    96T/48T
  • 價格:
    ¥3200/¥2500
  • 其他:

產(chǎn)品詳情

  • 產(chǎn)品描述:
    小鼠血管性血友病因子/瑞斯托霉素輔因子(vWF)酶聯(lián)免疫試劑盒(CSB-E08439m)為雙抗夾心法ELISA試劑盒,定量檢測血清、血漿、組織勻漿樣本中的VWF含量。VWF即血管性血友病因子,在止血過程中起關(guān)鍵作用,其缺陷會引發(fā)血管性血友病。研究機(jī)制方面,它可與血小板糖蛋白及內(nèi)皮下膠原結(jié)合,促進(jìn)血小板黏附聚集;還能作為載體蛋白結(jié)合Ⅷ因子,防止其被降解,在凝血途徑中發(fā)揮重要功能。試劑盒檢測范圍為0.78 ng/mL-50 ng/mL,為研究小鼠模型中的血管功能調(diào)控、出血性疾病機(jī)理、抗血栓藥物作用效果等提供可靠數(shù)據(jù)支持,尤其適用于心血管疾病動物模型、基因編輯小鼠的表型分析以及凝血相關(guān)生物標(biāo)志物的基礎(chǔ)研究本品僅用于科研,不用于臨床診斷,產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名:
    Vwfvon Willebrand factor ELISA kit; vWF) [Cleaved into: von Willebrand antigen 2 ELISA kit; von Willebrand antigen II)] ELISA kit
  • 縮寫:
  • Uniprot No.:
  • 種屬:
    Mus musculus (Mouse)
  • 樣本類型:
    serum, plasma, tissue homogenates
  • 檢測范圍:
    0.78 ng/mL-50 ng/mL
  • 靈敏度:
    0.195 ng/mL
  • 反應(yīng)時間:
    1-5h
  • 樣本體積:
    50-100ul
  • 檢測波長:
    450 nm
  • 研究領(lǐng)域:
    Cardiovascular
  • 測定原理:
    quantitative
  • 測定方法:
    Sandwich
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse vWF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
     SampleSerum(n=4)
    1:100Average %93
    Range %88-98
    1:200Average %97
    Range %92-101
    1:400Average %99
    Range %95-104
    1:800Average %92
    Range %87-96
  • 回收率:
    The recovery of mouse vWF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9590-100
    EDTA plasma (n=4)9387-98
  • 標(biāo)準(zhǔn)曲線:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/mlOD1OD2AverageCorrected
    502.323 2.451 2.387 2.293
    251.645 1.788 1.717 1.623
    12.51.067 1.021 1.044 0.950
    6.250.602 0.592 0.597 0.503
    3.120.283 0.302 0.293 0.199
    1.560.245 0.235 0.240 0.146
    0.780.166 0.169 0.168 0.074
    00.092 0.096 0.094  
  • 數(shù)據(jù)處理:
  • 貨期:
    3-5 working days

產(chǎn)品評價

靶點(diǎn)詳情

  • 功能:
    Important in the maintenance of hemostasis, it promotes adhesion of platelets to the sites of vascular injury by forming a molecular bridge between sub-endothelial collagen matrix and platelet-surface receptor complex GPIb-IX-V. Also acts as a chaperone for coagulation factor VIII, delivering it to the site of injury, stabilizing its heterodimeric structure and protecting it from premature clearance from plasma.
  • 基因功能參考文獻(xiàn):
    1. is synthesized in megakaryocytes and endothelial cells (ECs) and has two main roles: to carry and protect coagulation factor VIII (FVIII) from degradation by forming VWF-FVIII complex; and to mediate platelet adhesion and aggregation at sites of vascular injury. PMID: 29392565
    2. this study shows that Von Willebrand factor protects against acute CCl4-induced hepatotoxicity through phospho-p38 MAPK signaling pathway inhibition PMID: 28868583
    3. the ADAMTS13-vWF axis is partially involved in the pathophysiology of kidney ischemic reperfusion injury. PMID: 27507004
    4. Type 2N von Willebrand disease variants were associated with decreased VWF secretion and impaired factor VIII binding/stability. PMID: 28581694
    5. identify a critical role for VWF in cerebral inflammation and blood-brain barrier damage after intracerebral haemorrhage PMID: 27782211
    6. Refrigeration-induced binding of VWF to platelets facilitates their rapid clearance by inducing GPIbalpha-mediated signaling. PMID: 29097365
    7. BLOC-2 subunit HPS6 deficiency affects the tubulation and secretion of von Willebrand factor from mouse endothelial cells PMID: 27889498
    8. A novel single-domain antibody against von Willebrand factor A1 domain that interferes with VWF-platelet interactions in vivo. By using this sdAb, show that the A1 domain is pertinent to the participation of VWF in the inflammatory response. PMID: 28642239
    9. These experiments delineate an unexpected pathway in which microbiota-triggered TLR2 signaling alters the synthesis of proadhesive VWF by the liver endothelium and favors platelet integrin-dependent thrombus growth. PMID: 28572286
    10. ADAMTS13 controls vascular remodeling by modifying VWF reactivity during stroke recovery. PMID: 28428179
    11. these novel findings support the hypothesis that conformation of the VWF A domains plays a critical role in modulating macrophage-mediated clearance of VWF in vivo. PMID: 27554083
    12. results revealed localized vascular expression of FVIII and von Willebrand factor and identified lymphatic endothelial cell as a major cellular source of FVIII in extrahepatic tissues. PMID: 27207787
    13. Endothelial cell derivedVWF is the major determinant that mediates VWF-dependent ischemic stroke by promoting postischemic thrombo-inflammation. PMID: 27444201
    14. VWF deficiency reduces the progression of liver fibrosis, suggesting a mechanistic role of elevated plasma VWF levels in cirrhosis PMID: 28527913
    15. von Willebrand factor exerts beneficial effects in a mouse sepsis model via recruitment of neutrophils to inflammatory sites. PMID: 26494840
    16. Staphylococcus lugdunensis binds directly to von Willebrand factor, which proved to be vital for withstanding shear forces and for its adhesion to the vessel wall and cardiac valves. PMID: 26743845
    17. Clinical experimental cerebral malaria progression was delayed, and overall survival was significantly prolonged in VWF(-/-) mice compared with WT controls. PMID: 26511133
    18. in stable compensated heart failure mice, disruptions in endothelial vWF expression and extrusion may reduce the incidence of endocardial thrombosis PMID: 26565707
    19. VWF is expressed in a mosaic pattern in the capillaries of many vascular beds and in the aorta. Hearts of VWF-null mice demonstrate an abnormal endothelial phenotype as well as cardiac dysfunction. PMID: 26744078
    20. SNAP23 Regulates Endothelial Exocytosis of von Willebrand Factor PMID: 26266817
    21. Both platelet-VWF and plasma-VWF are required for optimal platelet-derived FVIII gene therapy for hemophilia A in the presence of inhibitors. PMID: 25955153
    22. a genetic link between EGLN1 and VWF in a constitution specific manner which could modulate thrombosis/bleeding susceptibility and outcomes of hypoxia, is reported. PMID: 26047609
    23. novel findings demonstrate a specific and critical role for the R1205 residue in modulating macrophage-mediated clearance of VWF in vivo PMID: 25690668
    24. Clearance differences between blood group O and non-blood group O individuals may therefore be related to the blood group status of the individual rather than the ABH antigen loading on VWF itself. PMID: 25650553
    25. Certain VWD-type 2B mutations relieve the need for shear stress to induce LRP1 binding. Enhanced LRP1 binding coincides with a reduced survival of VWF/p.R1306Q and VWF/p.V1316M PMID: 25728415
    26. These data suggest that whereas platelet-derived VWF does not play a crucial role in hemostasis and arterial thrombosis, it aggravates thrombo-inflammatory diseases such as stroke via a GPIb-dependent mechanism. PMID: 26209660
    27. Platelet-endothelial interactions occur in early atherosclerosis. These interactions are in part caused by endothelial von Willebrand factor large multimers, which can be reversed with exogenous ADAMTS13. PMID: 26156014
    28. Data suggest that targeting platelet receptor glycoprotein Ibalpha (GPIbalpha)-von Willebrand factor VWF-A1 binding interface may offer a therapeutic approach to reducing platelet-driven thrombosis. PMID: 25293780
    29. Absence of vWF increases hematopoiesis in long-term bone marrow cultures and has a protective effect in irradiated lungs. PMID: 24982209
    30. VWF-binding protein contributes to vascular adhesion of S aureus through 2 independent mechanisms: shear-mediated binding to VWF and activation of prothrombin to form S aureus-fibrin-platelet aggregates. PMID: 24951431
    31. a delayed and markedly reduced thrombogenic response was still evident in VWF(-/-), GPVI, and alpha2beta1 blocked animals, suggesting that alternative primary hemostatic mechanisms can partially rescue the bleeding phenotype associated with these defects. PMID: 25051961
    32. a fragment containing only approximately 20% of the VWF sequence is sufficient to support FVIII stability in vivo PMID: 24850761
    33. Expression of VWF/p.S1494C-p.A1534C in mice triggers an acute onset of thrombotic thrombocytopenic purpura. PMID: 24713928
    34. Following endothelial damage, platelet cross-linking during closure of the vessel lumen is mediated by GPIbalpha-VWF interactions. PMID: 24553181
    35. The results indicate that elevation of extracellular sodium within the physiological range raises vWF sufficiently to increase coagulability and risk of thrombosis. PMID: 24733925
    36. Galpha12 may play an important role in promoting membrane trafficking and exocytosis for basal and thrombin-induced vWF secretion, in a process involving SNAP, Galpha12/13 and Galphaq/11 PMID: 24081657
    37. Carboxyl terminus of ADAMTS13 directly inhibits platelet aggregation and ultra large von Willebrand factor string formation under flow in a free-thiol-dependent manner. PMID: 24357063
    38. VWF represents a promising target for the treatment of cutaneous inflammation, e.g., leukocytoclastic vasculitis. PMID: 23812299
    39. VWF has a role in leukocyte recruitment with chromatin decondensation by PAD4, which increases myocardial ischemia/reperfusion injury in mice PMID: 24200682
    40. Platelet aggregation, secretion, and spreading were diminished due to inhibition of integrin alphaIIbbeta3 in platelets from mice expressing a vWD-type 2B-associated vWF (vWF/p.V1316M). PMID: 24270421
    41. a new function for VWF in vivo as regulator of bloodstream thrombopoiesis PMID: 23737952
    42. VWF type 2B binds to platelets, which is a signal for clearance by macrophages, possibly contributing to thrombocytopenia PMID: 23945153
    43. VWF deficiency confers partial preservation of blood brain barrier integrity after hypoxia/reoxygenation and seizures. PMID: 23825365
    44. Compared with wild-type mice (n=6), we found less bacteria in postcapillary (60+/-6 versus 32+/-5 bacteria) and collecting venules (48+/-5 versus 18+/-4 bacteria; P<0.05) of VWF knockout mice (n=5). PMID: 23720451
    45. Hypoxia is associated with a phenotypic shift which target regulation of VWD in lung endothelial cells. PMID: 23580145
    46. These findings implicate a role for intronic splicing in mediating lineage-specific expression of vWF in the endothelium. PMID: 23529929
    47. ADAMTS13 and VWF are causally involved in myocardial ischemia/reperfusion injury. PMID: 22983446
    48. Provide new evidence for ADAMTS13 in reducing VWF-mediated acute cerebral inflammation following ischemic stroke. PMID: 22712744
    49. Endothelial cell (EC) VWF is sufficient to support hemostasis in VWF-/- mice, and VWF produced in megakaryocytes/platelets can also contribute to hemostasis in the absence of EC-derived VWF. PMID: 22642380
    50. study showed O-glycosylations are dispensable for normal VWF multimerization and biosynthesis; it appears that some O-glycosylation sites, particularly the T1255 and T1256 residues, are involved in maintenance of VWF plasma levels and are essential for normal haemostasis PMID: 22616016

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  • 亞細(xì)胞定位:
    Secreted. Secreted, extracellular space, extracellular matrix.
  • 組織特異性:
    Plasma. Expressed in liver.
  • 數(shù)據(jù)庫鏈接:


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