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Mouse sex hormone-binding globulin,SHBG ELISA Kit

  • 中文名稱:
    小鼠性激素結合球蛋白(SHBG)酶聯免疫試劑盒
  • 貨號:
    CSB-E08233m
  • 規格:
    96T/48T
  • 價格:
    ¥3800/¥2500
  • 其他:

產品詳情

  • 產品描述:
    小鼠性激素結合球蛋白(SHBG)酶聯免疫試劑盒(CSB-E08233m)為雙抗夾心法ELISA試劑盒,定量檢測血清、血漿樣本中的SHBG含量。SHBG即性激素結合球蛋白,它能特異性結合雄激素和雌激素,調控性激素在體內的活性。近年來研究發現其與多種疾病相關,如多囊卵巢綜合征、代謝綜合征等。深入研究其作用機制,或可助力相關疾病的早期診斷、病情評估及治療靶點開發。試劑盒檢測范圍為1.3 ng/mL-50 ng/mL,適用于研究性激素代謝調控機制、生殖系統疾病模型構建、內分泌干擾物評估等基礎科研場景,可滿足實驗室對小鼠生殖內分泌相關研究的定量檢測需求,為探索激素依賴性生理病理過程提供可靠工具。本品僅用于科研,不用于臨床診斷,產品具體參數及操作步驟詳見產品說明書。
  • 別名:
    Shbg ELISA Kit; Sex hormone-binding globulin ELISA Kit; SHBG ELISA Kit; Sex steroid-binding protein ELISA Kit; SBP ELISA Kit; Testis-specific androgen-binding protein ELISA Kit; ABP ELISA Kit
  • 縮寫:
    SHBG
  • Uniprot No.:
  • 種屬:
    Mus musculus (Mouse)
  • 樣本類型:
    serum, plasma
  • 檢測范圍:
    1.3 ng/mL-50 ng/mL
  • 靈敏度:
    0.33 ng/mL
  • 反應時間:
    1-5h
  • 樣本體積:
    50-100ul
  • 檢測波長:
    450 nm
  • 研究領域:
    Signal Transduction
  • 測定原理:
    quantitative
  • 測定方法:
    Sandwich
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<15%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<15%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse SHBG in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:1Average %91
    Range %86-97
    1:2Average %97
    Range %92-103
    1:4Average %85
    Range %81-90
    1:8Average %89
    Range %83-95
  • 回收率:
    The recovery of mouse SHBG spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9187-95
    EDTA plasma (n=4)109104-114
  • 標準曲線:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/mlOD1OD2AverageCorrected
    502.344 2.491 2.418 2.370
    271.768 1.822 1.795 1.747
    131.003 1.042 1.023 0.975
    3.30.376 0.365 0.371 0.323
    1.30.175 0.189 0.182 0.134
    00.046 0.049 0.048
  • 數據處理:
  • 貨期:
    3-5 working days

產品評價

靶點詳情

  • 功能:
    Functions as an androgen transport protein, but may also be involved in receptor mediated processes. Each dimer binds one molecule of steroid. Specific for 5-alpha-dihydrotestosterone, testosterone, and 17-beta-estradiol. Regulates the plasma metabolic clearance rate of steroid hormones by controlling their plasma concentration.
  • 基因功能參考文獻:
    1. SHBG suppresses inflammation and lipid accumulation in macrophages and adipocytes, which might be among the mechanisms underlying the protective effect of SHBG, that is, its actions which reduce the incidence of metabolic syndrome PMID: 30046278
    2. A wave of apoptosis during the pre-pubertal period is required for normal spermatogenesis to develop, and our data indicate that this apoptotic wave may be regulated by ABP and/or androgens. PMID: 12831397
    3. USF transcription factors repress SHBG transcription in Sertoli cells through an interaction with FP4 within its proximal promoter PMID: 15574421
    4. monosaccharide-induced lipogenesis reduced hepatic HNF-4alpha levels, which in turn attenuated SHBG expression. PMID: 17992261
  • 亞細胞定位:
    Secreted.
  • 數據庫鏈接:

    KEGG: mmu:20415

    STRING: 10090.ENSMUSP00000005334

    UniGene: Mm.1431



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