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中文名稱:小鼠纖溶酶抗纖溶酶復合物(PAP)酶聯免疫試劑盒
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貨號:CSB-E08436m
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規格:96T/48T
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價格:¥3200/¥2500
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其他:
產品詳情
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產品描述:小鼠纖溶酶抗纖溶酶復合物(PAP)酶聯免疫試劑盒(CSB-E08436m)為雙抗夾心法ELISA試劑盒,定量檢測血清、血漿、組織勻漿樣本中的PAP含量。試劑盒檢測范圍為3.12 pg/mL-200 pg/mL,適用于體外實驗環境下檢測小鼠模型中與血栓形成、溶栓治療或炎癥反應相關的PAP動態變化,例如在血栓性疾病模型評估、抗凝藥物作用機制研究或纖溶系統調控實驗中定量分析樣本生物標志物水平。本品僅用于科研,不用于臨床診斷,產品具體參數及操作步驟詳見產品說明書。
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別名:N/A
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縮寫:PAP
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種屬:Mus musculus (Mouse)
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樣本類型:serum, plasma, tissue homogenates
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檢測范圍:3.12 pg/mL-200 pg/mL
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靈敏度:0.78 pg/mL
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反應時間:1-5h
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樣本體積:50-100ul
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檢測波長:450 nm
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研究領域:Others
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測定原理:quantitative
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測定方法:Sandwich
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精密度:
Intra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess. -
線性度:
To assess the linearity of the assay, samples were spiked with high concentrations of mouse PAP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. Sample Serum(n=4) 1:5 Average % 87 Range % 83-94 1:10 Average % 97 Range % 92-102 1:20 Average % 105 Range % 98-111 1:40 Average % 93 Range % 87-99 -
回收率:
The recovery of mouse PAP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 92 88-96 EDTA plasma (n=4) 101 95-107 -
標準曲線:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. pg/ml OD1 OD2 Average Corrected 200 2.245 2.264 2.255 2.072 100 1.805 1.855 1.830 1.647 50 1.178 1.155 1.167 0.984 25 0.739 0.796 0.768 0.585 12.5 0.398 0.421 0.410 0.227 6.25 0.312 0.335 0.324 0.141 3.12 0.259 0.248 0.254 0.071 0 0.188 0.178 0.183 -
數據處理:
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貨期:3-5 working days
引用文獻
- Role of platelets in liver damage and regeneration FWB Reusswig,/,2021
- The fibrinolytic factor tPA drives LRP1-mediated melanoma growth and metastasis Salama Y, et al,Faseb journal,2018
- The role of plasmin in the pathogenesis of murine multiple myeloma Eiamboonsert S.et al,Biochem Biophys Res Commun.,2017
- Plasminogen activator inhibitor-1 regulates macrophage-dependent postoperative adhesion by enhancing EGF-HER1 signaling in mice Kumpei Honjo.et al,FASEB J.,2017
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