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Mouse periostin/osteoblast specific factor 2 (POSTN) ELISA kit

  • 中文名稱:
    小鼠骨膜蛋白/成骨細(xì)胞特異性因子2(POSTN)酶聯(lián)免疫試劑盒
  • 貨號(hào):
    CSB-EL018381MO
  • 規(guī)格:
    96T/48T
  • 價(jià)格:
    ¥3600/¥2500
  • 其他:

產(chǎn)品詳情

  • 產(chǎn)品描述:
    小鼠骨膜蛋白/成骨細(xì)胞特異性因子2(POSTN)酶聯(lián)免疫試劑盒(CSB-EL018381MO)為雙抗夾心法ELISA試劑盒,定量檢測(cè)血清、血漿、組織勻漿樣本中的POSTN含量。POSTN即骨膜蛋白,是一種細(xì)胞外基質(zhì)蛋白。其在多種生理病理過(guò)程發(fā)揮作用,如胚胎發(fā)育、組織修復(fù)等。研究機(jī)制上,它可與多種細(xì)胞表面受體相互作用,激活下游信號(hào)通路,影響細(xì)胞增殖、遷移、黏附等,且在腫瘤等疾病中異常表達(dá)。試劑盒檢測(cè)范圍為1.56 ng/mL-100 ng/mL,靈敏度為0.039 ng/mL。適用于骨損傷修復(fù)、纖維化疾病模型(如肺纖維化、心肌纖維化)、腫瘤微環(huán)境研究等領(lǐng)域的機(jī)制探索,也可用于骨骼發(fā)育相關(guān)基因敲除或轉(zhuǎn)基因動(dòng)物模型中POSTN表達(dá)水平的動(dòng)態(tài)監(jiān)測(cè)。本品僅用于科研,不用于臨床診斷,產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書。
  • 別名:
    Postn ELISA Kit; Osf2 ELISA Kit; Periostin ELISA Kit; PN ELISA Kit; Osteoblast-specific factor 2 ELISA Kit; OSF-2 ELISA Kit
  • 縮寫:
  • Uniprot No.:
  • 種屬:
    Mus musculus (Mouse)
  • 樣本類型:
    serum, plasma, tissue homogenates
  • 檢測(cè)范圍:
    1.56 ng/mL-100 ng/mL
  • 靈敏度:
    0.039 ng/mL
  • 反應(yīng)時(shí)間:
    1-5h
  • 樣本體積:
    50-100ul
  • 檢測(cè)波長(zhǎng):
    450 nm
  • 研究領(lǐng)域:
    Cardiovascular
  • 測(cè)定原理:
    quantitative
  • 測(cè)定方法:
    Sandwich
  • 精密度:

    Intra-assay Precision (Precision within an assay): CV%<8%

    Three samples of known concentration were tested twenty times on one plate to assess.

    Inter-assay Precision (Precision between assays): CV%<10%

    Three samples of known concentration were tested in twenty assays to assess.

     

    Intra-Assay Precision

    Inter-Assay Precision

    Sample

    1

    2

    3

    1

    2

    3

    n

    20

    20

    20

    20

    20

    20

    Mean(ng/ml)

    12.563

    12.652

    12.759

    12.706

    12.546

    12.457

    SD

    0.037

    0.035

    0.043

    0.058

    0.054

    0.060

    CV(%)

    3.978

    3.743

    4.570

    6.183

    5.813

    6.494

  • 線性度:

    To assess the linearity of the assay, samples were spiked with high concentrations of mouse OSF-2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

     

    Sample

    Serum(n=4)

    1:20

    Average %

    103

    Range %

    94-107

    1:40

    Average %

    96

    Range %

    85-100

    1:80

    Average %

    91

    Range %

    85-95

    1:160

    Average %

    91

    Range %

    82-94

  • 回收率:

    The recovery of mouse OSF-2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample Type

    Average % Recovery

    Range%

    Serum (n=5)

    92

    84-96

    EDTA plasma (n=4)

    90

    83-95

  • 標(biāo)準(zhǔn)曲線:

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

    ng/ml

    OD1

    OD2

    Average

    Corrected

    100

    2.511

    2.435

    2.473

    2.299

    50

    2.101

    2.075

    2.088

    1.914

    25

    1.452

    1.499

    1.476

    1.302

    12.5

    0.925

    0.938

    0.932

    0.758

    6.25

    0.549

    0.573

    0.561

    0.387

    3.13

    0.402

    0.414

    0.408

    0.234

    1.56

    0.284

    0.293

    0.289

    0.115

    0

    0.172

    0.176

    0.174

     

  • 數(shù)據(jù)處理:
  • 貨期:
    3-5 working days

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 最新研究進(jìn)展:
    POSTN是一種細(xì)胞外基質(zhì)蛋白,最近的研究表明它在人體內(nèi)的生理過(guò)程中起到重要的作用。POSTN在骨骼肌肌肉再生和維持中有重要作用,同時(shí)也參與了心血管、肺部和神經(jīng)系統(tǒng)的生理過(guò)程,還有可能參與腫瘤的發(fā)生和發(fā)展。未來(lái)的研究將進(jìn)一步揭示POSTN在生理和病理狀態(tài)下的作用機(jī)制,為開(kāi)發(fā)新的治療策略提供理論依據(jù)。
  • 功能:
    Induces cell attachment and spreading and plays a role in cell adhesion. Enhances incorporation of BMP1 in the fibronectin matrix of connective tissues, and subsequent proteolytic activation of lysyl oxidase LOX.
  • 基因功能參考文獻(xiàn):
    1. Inhibition of periostin could be important for suppressing capsule formation on silicone implants after in vivo implantation. PMID: 29854742
    2. Unaffected by Postn, Vav-Itgav(-/-) haematopoietic stem cell proliferate faster in vitro, illustrating the importance of Postn-Itgav interaction. PMID: 27905395
    3. mesenchymal source, specific to cardiovascular tissues, roles in cardiovascular development and disease [review] PMID: 29101484
    4. Results indicate that periostin is a key player in the development of bleomycin-induced fibrosis and consequent enhancement of tumor cell colonization in the lung. PMID: 29498146
    5. Periostin gene deletion impairs periosteal cells functions and fracture consolidation. PMID: 29472541
    6. these data indicate that CatK not only plays a major role in bone remodeling but also modulates modeling-based cortical bone formation by degrading periostin and thereby moderating Wnt-beta-catenin signaling. PMID: 28322464
    7. Ablation of periostin suppresses post-infarction myocardial regeneration by inhibiting the PI3K/GSK3beta/cyclin D1 signalling pathway, indicating that periostin is essential for myocardial regeneration. PMID: 28453729
    8. this study shows that periostin regulates fibrocyte function to promote myofibroblast differentiation and lung fibrosis PMID: 27435108
    9. Genetic or pharmaco-genetic inhibition of periostin in animal models of renal disease was efficient in arresting the progression of the kidney pathology. (Review) PMID: 28884334
    10. TGFBI and periostin act similarly in the heart in affecting fibrosis and disease responsiveness. PMID: 28750100
    11. Results suggest that stromal cell periostin (POSTN) supports both normal hematopoietic progenitor cells (HPCs) and leukemia-initiating cells in vitro. PMID: 27596966
    12. the expressions of the periostin splice variants were increased in ischemic retinas. PMID: 27744020
    13. POSTN regulates the scar formation after spinal cord injuries. PMID: 28082119
    14. periostin in the extracellular matrix architecture prevents pulmonary leakage of blood fluid, thus increasing the survival rate in mice with acute lung injury PMID: 27270966
    15. These results indicated that tensile stress load increased the expression levels of PN in mouse peridontal ligaments and human periodontal ligament cells. PMID: 28260019
    16. POSTN promotes the pulmonary accumulation of myeloid-derived suppressor cells (MDSCs) during the early stage of breast tumour metastasis. Postn deletion decreases neutrophil and monocytic cell populations in the bone marrow of mice and suppresses the accumulation of MDSCs to premetastatic sites. PMID: 27193093
    17. Fibrillin-2 and periostin are target genes in Osterix-mediated osteoblast differentiation. PMID: 27751812
    18. Periostin mediates intestinal inflammation through the activation of NF-kappaB signaling, which suggests that periostin is a potential therapeutic target for inflammatory bowel disease. PMID: 26890265
    19. Thus, these results demonstrate a subcellular role of periostin in promotion of fibronectin secretion from the ER. PMID: 26820539
    20. These data demonstrate that the expression of periostin is stimulated by ErbB ligand NRG1 and influences the migration of Schwann cell precursors. PMID: 26187852
    21. Our data suggest that POSTN is a crucial factor for proper exocrine lineage-specific regeneration after severe acute pancreatitis. PMID: 26632158
    22. Periostin functions as a regulator of atherosclerotic lesion formation and progression. PMID: 26564821
    23. HMGB1 expression in the periodontal ligament is differentially regulated during orthodontic tooth movement in wild type versus periostin-deficient mice. PMID: 25948513
    24. POSTN was expressed in the region surrounding melanoma cell nests in metastatic lesions of both wounded mice and the patient. PMID: 26083413
    25. POSTN significantly enhances neural stem cell proliferation and differentiation after hypoxia-ischemia. PMID: 25894199
    26. loss of Postn in an ErbB2/Neu/HER2 overexpression model results in apocrine-like tumors that activate an AR-dependent pathway. PMID: 25592291
    27. Periostin appears to be a mediator for epidermal hyperplasia that is common to AD and psoriasis. PMID: 25572557
    28. Deficiency in periostin abrogated the development of liver fibrosis in mice. PMID: 25541330
    29. key pathologic factors responsible for bone and periodontal ligament damage in periostin-null mice PMID: 25757567
    30. These results further indicate that periostin is an essential mediator of cortical bone response to mechanical forces (loading and unloading). PMID: 25445447
    31. This study found that the secreted protein periostin (Postn) is highly induced after partial pancreatectomy in regenerating areas containing mesenchymal stroma and tubular complexes. PMID: 25485969
    32. Periostin might be associated with pathophysiology in post-ischemic inflammation and neurogenesis. PMID: 24646642
    33. Overexpression of periostin resulted in reduced expression of peroxisome proliferator-activated receptor alpha (PPARalpha), a master regulator of fatty acid oxidation, and activation of the JNK signaling pathway. PMID: 25003192
    34. In mice, periostin is required for maximal airways hyperresponsiveness and inflammation after house dust mite sensitization and challenge. PMID: 24996263
    35. periostin deficiency may not only cause periodontal ligament collagen fibril disorganization, but could also affect the distribution of other major extracellular matrix proteins such as fibronectin and tenascin C. PMID: 24352874
    36. periostin plays an essential role in the function of collagenolytic enzymes like cathepsin K, MMP1 and MMP2 in the compressed PDL after orthodontic force application. PMID: 24202437
    37. adversely modifies the progression of renal cystic disease by promoting cyst epithelial cell proliferation, cyst enlargement, and interstitial fibrosis PMID: 24284511
    38. Periostin-induced epithelial migration and proliferation requires mTOR signaling. PMID: 24349533
    39. Periostin and a paracrine loop composed of IL-1alpha and IL-6 regulates keratinocyte proliferation and differentiation in the epithelial-mesenchymal interactions, and periostin tunes the magnitude of keratinocyte proliferation and differentiation. PMID: 24352037
    40. Periostin/beta-INTEGRINs/focal adhesion kinase/PI3K/ERK signals promote hyaluronan synthase-2 activation, matrix remodeling, and valve progenitor cell survival/differentiation. PMID: 24469446
    41. the level of periostin expression could influence the propensity to fatigue fractures. PMID: 24167618
    42. Periostin downregulation is an early marker of inhibited neonatal murine lung alveolar septation. PMID: 23723163
    43. Metabolic activity of CYP1B1 contributes to oxidative homeostasis and ultrastructural organization and function of trabecular meshwork tissue through modulation of Postn expression. PMID: 23979599
    44. Clear cell renal cell carcinoma induces fibroblast-mediated accumulation of stromal periostin, enhancing tumor cell attachment and fibroblast proliferation. PMID: 23896380
    45. periostin exacerbates the pathogenesis of AD through TSLP production from keratinocytes. PMID: 22918211
    46. Periostin plays an essential role in the pathogenesis of Bleomycin-induced scleroderma in mice. PMID: 22911870
    47. These results suggest that periostin, which is secreted by the intraocular macrophages, enhances the hyaloidvascular system regression by intensifying the adhesion of macrophages to hyaloid vessels. PMID: 22930727
    48. Periostin regulates beta catenin signaling and parathyroid hormone anabolic effects in bone. PMID: 22927401
    49. Periostin overexpression is associated with kidney injury. PMID: 22167593
    50. periostin sets up a vicious circle that links Th2-type immune responses to keratinocyte activation and plays a critical role in the amplification and chronicity of allergic skin inflammation. PMID: 22684102

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  • 亞細(xì)胞定位:
    Golgi apparatus. Secreted. Secreted, extracellular space, extracellular matrix.
  • 組織特異性:
    Preferentially expressed in periosteum and periodontal ligament. Also expressed in the developing and adult heart.
  • 數(shù)據(jù)庫(kù)鏈接:


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