Mouse periostin/osteoblast specific factor 2 (POSTN) ELISA kit
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中文名稱:小鼠骨膜蛋白/成骨細(xì)胞特異性因子2(POSTN)酶聯(lián)免疫試劑盒
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貨號(hào):CSB-EL018381MO
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規(guī)格:96T/48T
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價(jià)格:¥3600/¥2500
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其他:
產(chǎn)品詳情
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產(chǎn)品描述:小鼠骨膜蛋白/成骨細(xì)胞特異性因子2(POSTN)酶聯(lián)免疫試劑盒(CSB-EL018381MO)為雙抗夾心法ELISA試劑盒,定量檢測(cè)血清、血漿、組織勻漿樣本中的POSTN含量。POSTN即骨膜蛋白,是一種細(xì)胞外基質(zhì)蛋白。其在多種生理病理過(guò)程發(fā)揮作用,如胚胎發(fā)育、組織修復(fù)等。研究機(jī)制上,它可與多種細(xì)胞表面受體相互作用,激活下游信號(hào)通路,影響細(xì)胞增殖、遷移、黏附等,且在腫瘤等疾病中異常表達(dá)。試劑盒檢測(cè)范圍為1.56 ng/mL-100 ng/mL,靈敏度為0.039 ng/mL。適用于骨損傷修復(fù)、纖維化疾病模型(如肺纖維化、心肌纖維化)、腫瘤微環(huán)境研究等領(lǐng)域的機(jī)制探索,也可用于骨骼發(fā)育相關(guān)基因敲除或轉(zhuǎn)基因動(dòng)物模型中POSTN表達(dá)水平的動(dòng)態(tài)監(jiān)測(cè)。本品僅用于科研,不用于臨床診斷,產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書。
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別名:Postn ELISA Kit; Osf2 ELISA Kit; Periostin ELISA Kit; PN ELISA Kit; Osteoblast-specific factor 2 ELISA Kit; OSF-2 ELISA Kit
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縮寫:
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Uniprot No.:
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種屬:Mus musculus (Mouse)
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樣本類型:serum, plasma, tissue homogenates
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檢測(cè)范圍:1.56 ng/mL-100 ng/mL
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靈敏度:0.039 ng/mL
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反應(yīng)時(shí)間:1-5h
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樣本體積:50-100ul
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檢測(cè)波長(zhǎng):450 nm
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研究領(lǐng)域:Cardiovascular
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測(cè)定原理:quantitative
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測(cè)定方法:Sandwich
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精密度:
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Intra-Assay Precision
Inter-Assay Precision
Sample
1
2
3
1
2
3
n
20
20
20
20
20
20
Mean(ng/ml)
12.563
12.652
12.759
12.706
12.546
12.457
SD
0.037
0.035
0.043
0.058
0.054
0.060
CV(%)
3.978
3.743
4.570
6.183
5.813
6.494
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線性度:
To assess the linearity of the assay, samples were spiked with high concentrations of mouse OSF-2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
Sample
Serum(n=4)
1:20
Average %
103
Range %
94-107
1:40
Average %
96
Range %
85-100
1:80
Average %
91
Range %
85-95
1:160
Average %
91
Range %
82-94
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回收率:
The recovery of mouse OSF-2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type
Average % Recovery
Range%
Serum (n=5)
92
84-96
EDTA plasma (n=4)
90
83-95
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標(biāo)準(zhǔn)曲線:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml
OD1
OD2
Average
Corrected
100
2.511
2.435
2.473
2.299
50
2.101
2.075
2.088
1.914
25
1.452
1.499
1.476
1.302
12.5
0.925
0.938
0.932
0.758
6.25
0.549
0.573
0.561
0.387
3.13
0.402
0.414
0.408
0.234
1.56
0.284
0.293
0.289
0.115
0
0.172
0.176
0.174
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數(shù)據(jù)處理:
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貨期:3-5 working days
相關(guān)產(chǎn)品
靶點(diǎn)詳情
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最新研究進(jìn)展:POSTN是一種細(xì)胞外基質(zhì)蛋白,最近的研究表明它在人體內(nèi)的生理過(guò)程中起到重要的作用。POSTN在骨骼肌肌肉再生和維持中有重要作用,同時(shí)也參與了心血管、肺部和神經(jīng)系統(tǒng)的生理過(guò)程,還有可能參與腫瘤的發(fā)生和發(fā)展。未來(lái)的研究將進(jìn)一步揭示POSTN在生理和病理狀態(tài)下的作用機(jī)制,為開(kāi)發(fā)新的治療策略提供理論依據(jù)。
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功能:Induces cell attachment and spreading and plays a role in cell adhesion. Enhances incorporation of BMP1 in the fibronectin matrix of connective tissues, and subsequent proteolytic activation of lysyl oxidase LOX.
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基因功能參考文獻(xiàn):
- Inhibition of periostin could be important for suppressing capsule formation on silicone implants after in vivo implantation. PMID: 29854742
- Unaffected by Postn, Vav-Itgav(-/-) haematopoietic stem cell proliferate faster in vitro, illustrating the importance of Postn-Itgav interaction. PMID: 27905395
- mesenchymal source, specific to cardiovascular tissues, roles in cardiovascular development and disease [review] PMID: 29101484
- Results indicate that periostin is a key player in the development of bleomycin-induced fibrosis and consequent enhancement of tumor cell colonization in the lung. PMID: 29498146
- Periostin gene deletion impairs periosteal cells functions and fracture consolidation. PMID: 29472541
- these data indicate that CatK not only plays a major role in bone remodeling but also modulates modeling-based cortical bone formation by degrading periostin and thereby moderating Wnt-beta-catenin signaling. PMID: 28322464
- Ablation of periostin suppresses post-infarction myocardial regeneration by inhibiting the PI3K/GSK3beta/cyclin D1 signalling pathway, indicating that periostin is essential for myocardial regeneration. PMID: 28453729
- this study shows that periostin regulates fibrocyte function to promote myofibroblast differentiation and lung fibrosis PMID: 27435108
- Genetic or pharmaco-genetic inhibition of periostin in animal models of renal disease was efficient in arresting the progression of the kidney pathology. (Review) PMID: 28884334
- TGFBI and periostin act similarly in the heart in affecting fibrosis and disease responsiveness. PMID: 28750100
- Results suggest that stromal cell periostin (POSTN) supports both normal hematopoietic progenitor cells (HPCs) and leukemia-initiating cells in vitro. PMID: 27596966
- the expressions of the periostin splice variants were increased in ischemic retinas. PMID: 27744020
- POSTN regulates the scar formation after spinal cord injuries. PMID: 28082119
- periostin in the extracellular matrix architecture prevents pulmonary leakage of blood fluid, thus increasing the survival rate in mice with acute lung injury PMID: 27270966
- These results indicated that tensile stress load increased the expression levels of PN in mouse peridontal ligaments and human periodontal ligament cells. PMID: 28260019
- POSTN promotes the pulmonary accumulation of myeloid-derived suppressor cells (MDSCs) during the early stage of breast tumour metastasis. Postn deletion decreases neutrophil and monocytic cell populations in the bone marrow of mice and suppresses the accumulation of MDSCs to premetastatic sites. PMID: 27193093
- Fibrillin-2 and periostin are target genes in Osterix-mediated osteoblast differentiation. PMID: 27751812
- Periostin mediates intestinal inflammation through the activation of NF-kappaB signaling, which suggests that periostin is a potential therapeutic target for inflammatory bowel disease. PMID: 26890265
- Thus, these results demonstrate a subcellular role of periostin in promotion of fibronectin secretion from the ER. PMID: 26820539
- These data demonstrate that the expression of periostin is stimulated by ErbB ligand NRG1 and influences the migration of Schwann cell precursors. PMID: 26187852
- Our data suggest that POSTN is a crucial factor for proper exocrine lineage-specific regeneration after severe acute pancreatitis. PMID: 26632158
- Periostin functions as a regulator of atherosclerotic lesion formation and progression. PMID: 26564821
- HMGB1 expression in the periodontal ligament is differentially regulated during orthodontic tooth movement in wild type versus periostin-deficient mice. PMID: 25948513
- POSTN was expressed in the region surrounding melanoma cell nests in metastatic lesions of both wounded mice and the patient. PMID: 26083413
- POSTN significantly enhances neural stem cell proliferation and differentiation after hypoxia-ischemia. PMID: 25894199
- loss of Postn in an ErbB2/Neu/HER2 overexpression model results in apocrine-like tumors that activate an AR-dependent pathway. PMID: 25592291
- Periostin appears to be a mediator for epidermal hyperplasia that is common to AD and psoriasis. PMID: 25572557
- Deficiency in periostin abrogated the development of liver fibrosis in mice. PMID: 25541330
- key pathologic factors responsible for bone and periodontal ligament damage in periostin-null mice PMID: 25757567
- These results further indicate that periostin is an essential mediator of cortical bone response to mechanical forces (loading and unloading). PMID: 25445447
- This study found that the secreted protein periostin (Postn) is highly induced after partial pancreatectomy in regenerating areas containing mesenchymal stroma and tubular complexes. PMID: 25485969
- Periostin might be associated with pathophysiology in post-ischemic inflammation and neurogenesis. PMID: 24646642
- Overexpression of periostin resulted in reduced expression of peroxisome proliferator-activated receptor alpha (PPARalpha), a master regulator of fatty acid oxidation, and activation of the JNK signaling pathway. PMID: 25003192
- In mice, periostin is required for maximal airways hyperresponsiveness and inflammation after house dust mite sensitization and challenge. PMID: 24996263
- periostin deficiency may not only cause periodontal ligament collagen fibril disorganization, but could also affect the distribution of other major extracellular matrix proteins such as fibronectin and tenascin C. PMID: 24352874
- periostin plays an essential role in the function of collagenolytic enzymes like cathepsin K, MMP1 and MMP2 in the compressed PDL after orthodontic force application. PMID: 24202437
- adversely modifies the progression of renal cystic disease by promoting cyst epithelial cell proliferation, cyst enlargement, and interstitial fibrosis PMID: 24284511
- Periostin-induced epithelial migration and proliferation requires mTOR signaling. PMID: 24349533
- Periostin and a paracrine loop composed of IL-1alpha and IL-6 regulates keratinocyte proliferation and differentiation in the epithelial-mesenchymal interactions, and periostin tunes the magnitude of keratinocyte proliferation and differentiation. PMID: 24352037
- Periostin/beta-INTEGRINs/focal adhesion kinase/PI3K/ERK signals promote hyaluronan synthase-2 activation, matrix remodeling, and valve progenitor cell survival/differentiation. PMID: 24469446
- the level of periostin expression could influence the propensity to fatigue fractures. PMID: 24167618
- Periostin downregulation is an early marker of inhibited neonatal murine lung alveolar septation. PMID: 23723163
- Metabolic activity of CYP1B1 contributes to oxidative homeostasis and ultrastructural organization and function of trabecular meshwork tissue through modulation of Postn expression. PMID: 23979599
- Clear cell renal cell carcinoma induces fibroblast-mediated accumulation of stromal periostin, enhancing tumor cell attachment and fibroblast proliferation. PMID: 23896380
- periostin exacerbates the pathogenesis of AD through TSLP production from keratinocytes. PMID: 22918211
- Periostin plays an essential role in the pathogenesis of Bleomycin-induced scleroderma in mice. PMID: 22911870
- These results suggest that periostin, which is secreted by the intraocular macrophages, enhances the hyaloidvascular system regression by intensifying the adhesion of macrophages to hyaloid vessels. PMID: 22930727
- Periostin regulates beta catenin signaling and parathyroid hormone anabolic effects in bone. PMID: 22927401
- Periostin overexpression is associated with kidney injury. PMID: 22167593
- periostin sets up a vicious circle that links Th2-type immune responses to keratinocyte activation and plays a critical role in the amplification and chronicity of allergic skin inflammation. PMID: 22684102
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亞細(xì)胞定位:Golgi apparatus. Secreted. Secreted, extracellular space, extracellular matrix.
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組織特異性:Preferentially expressed in periosteum and periodontal ligament. Also expressed in the developing and adult heart.
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