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  4. Mouse neuregulin-4 (NRG4) ELISA kit

Mouse neuregulin-4 (NRG4) ELISA kit

  • 中文名稱：
    小鼠神經調節蛋白4(NRG4)酶聯免疫試劑盒
  • 貨號：
    CSB-EL016080MO
  • 規格：
    96T/48T
  • 價格：
    ￥3600/￥2500
  • 其他：

產品詳情

  • 產品描述：
    小鼠神經調節蛋白4(NRG4)酶聯免疫試劑盒（CSB-EL016080MO）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的NRG4含量。試劑盒檢測范圍為3.12 ng/mL-200 ng/mL，適用于科研領域中小鼠模型的基礎代謝研究、脂肪組織功能評估或相關疾病機制探索，為體外實驗提供高效可靠的工具。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
  • 別名：
    Nrg4 ELISA Kit; Pro-neuregulin-4 ELISA Kit; membrane-bound isoform ELISA Kit; Pro-NRG4) [Cleaved into: Neuregulin-4 ELISA Kit; NRG-4)] ELISA Kit
  • 縮寫：
  • Uniprot No.：
  • 種屬：
    Mus musculus (Mouse)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測范圍：
    3.12 ng/mL-200 ng/mL
  • 靈敏度：
    0.78 ng/mL
  • 反應時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領域：
    Neuroscience
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse NRG4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)
    1:1 Average % 97
    Range % 92-103
    1:2 Average % 91
    Range % 84-96
    1:4 Average % 90
    Range % 87-95
    1:8 Average % 100
    Range % 95-105
  • 回收率：
    The recovery of mouse NRG4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range
    Serum (n=5) 90 87-93
    EDTA plasma (n=4) 94 90-98
  • 標準曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average Corrected
    200 2.549 2.763 2.656 2.556
    100 2.135 2.131 2.133 2.033
    50 1.587 1.622 1.605 1.505
    25 1.014 1.033 1.024 0.924
    12.5 0.736 0.787 0.762 0.662
    6.25 0.468 0.436 0.452 0.352
    3.12 0.214 0.231 0.223 0.123
    0 0.101 0.098 0.100 　
  • 數據處理：
  • 貨期：
    3-5 working days

引用文獻

產品評價

靶點詳情

  • 功能：
    Low affinity ligand for the ERBB4 tyrosine kinase receptor. Concomitantly recruits ERBB1 and ERBB2 coreceptors, resulting in ligand-stimulated tyrosine phosphorylation and activation of the ERBB receptors. Does not bind to the ERBB1, ERBB2 and ERBB3 receptors.
  • 基因功能參考文獻：
    1. The found of this study showed that NRG4 is a major novel regulator of dendritic arborisation in the developing cerebral cortex and suggest that it exerts its effects by an autocrine/paracrine mechanism. PMID: 29317193
    2. Conditional knockout of Nrg4 in brown adipocytes caused blood vessel reduction in brown but not in white adipose tissue , and was sufficient to induce obese phenotype. PMID: 29902456
    3. Nrg4 exerts beneficial effects on energy balance and glucose and lipid metabolism to ameliorate obesity-associated metabolic disorders. PMID: 28752050
    4. overexpression of the Nrg4 gene by hydrodynamic gene delivery prevents high fat diet-induced weight gain and fatty liver, alleviates obesity-induced chronic inflammation and insulin resistance PMID: 27184920
    5. Pharmacological inhibition of PI3K/Akt signaling reversed the anti-apoptotic effects of NRG4, confirming the role of this cascade in NRG4-induced cell survival PMID: 23033483

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  • 亞細胞定位：
    [Pro-neuregulin-4, membrane-bound isoform]: Cell membrane; Single-pass type I membrane protein.; [Neuregulin-4]: Secreted.
  • 蛋白家族：
    Neuregulin family
  • 組織特異性：
    Highly expressed in pancreas; weakly expressed in muscle.
  • 數據庫鏈接：


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