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Mouse mucin-5 subtype B,MUC5B ELISA Kit

  • 中文名稱:
    小鼠粘蛋白/粘液素5B(MUC5B)酶聯免疫試劑盒
  • 貨號:
    CSB-E15067m
  • 規格:
    96T/48T
  • 價格:
    ¥3600/¥2500
  • 其他:

產品詳情

  • 產品描述:
    小鼠粘蛋白/粘液素5B(MUC5B)酶聯免疫試劑盒(CSB-E15067m)為雙抗夾心法ELISA試劑盒,定量檢測血清、血漿、組織培養上清液、組織勻漿樣本中的MUC5B含量。MUC5B是重要的黏液蛋白靶點。其背景在于它在呼吸道黏液分泌中起關鍵作用,異常表達與多種呼吸道疾病如哮喘、慢性阻塞性肺疾病等相關。研究機制主要聚焦于調控其表達與分泌的信號通路,以探索干預策略,改善疾病黏液高分泌狀況。試劑盒檢測范圍為1.25 ng/mL-80 ng/mL,適用于體外實驗研究,如探究呼吸道疾病模型中黏液分泌調控機制、胃腸黏膜屏障功能評估、腫瘤微環境中黏蛋白表達分析等科研場景,為炎癥性疾病、黏膜相關病理生理學研究提供可靠工具。本品僅用于科研,不用于臨床診斷,產品具體參數及操作步驟詳見產品說明書。
  • 縮寫:
  • 種屬:
    Mus musculus (Mouse)
  • 樣本類型:
    serum, plasma, cell culture supernates, tissue homogenates
  • 檢測范圍:
    1.25 ng/mL-80 ng/mL
  • 靈敏度:
    0.31 ng/mL
  • 反應時間:
    1-5h
  • 樣本體積:
    50-100ul
  • 檢測波長:
    450 nm
  • 研究領域:
    Signal Transduction
  • 測定原理:
    quantitative
  • 測定方法:
    Sandwich
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse MUC5B in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
     SampleSerum(n=4)
    1:1Average %87
    Range %81-94
    1:2Average %96
    Range %91-102
    1:4Average %99
    Range %93-105
    1:8Average %92
    Range %86-98
  • 回收率:
    The recovery of mouse MUC5B spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9387-96
    EDTA plasma (n=4)9890-105
  • 標準曲線:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/mlOD1OD2AverageCorrected
    802.236 2.141 2.189 2.098
    401.879 1.801 1.840 1.749
    201.461 1.369 1.415 1.324
    100.853 0.793 0.823 0.732
    50.512 0.496 0.504 0.413
    2.50.307 0.301 0.304 0.213
    1.250.195 0.186 0.191 0.100
    00.093 0.088 0.091  
  • 數據處理:
  • 貨期:
    3-5 working days

產品評價

平均分:
5.0分 - 2 個評價

樣品類型:組織勻漿液 肺組織勻漿上清

樣品信息:小鼠

稀釋比:沒有稀釋

產品評價: 我通過使用CSB-E15067m檢測了PR8病毒感染的小鼠肺組織勻漿上清,操作簡單方便易上手,靈敏度非常高,檢測結果十分精準

By 鄧老師

樣品類型:組織勻漿液 肺組織勻漿上清

樣品信息:小鼠

稀釋比:沒有稀釋

產品評價: 我通過使用CSB-E15067m檢測了PR8病毒感染的小鼠肺組織勻漿上清,操作簡單方便易上手,靈敏度非常高,檢測結果十分精準

By 鄧老師