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Mouse malonyl coenzyme A ELISA kit

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產品描述：

小鼠丙二酸單酰輔酶A(malonyl CoA)酶聯免疫試劑盒（CSB-E12896m）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的malonyl CoA含量。Malonyl CoA是一種重要的代謝中間產物。它參與脂肪酸合成與氧化的調控，在能量代謝平衡中起關鍵作用。研究主要聚焦其在調控肉堿棕櫚酰轉移酶1活性以調節脂肪酸進入線粒體氧化的機制，與代謝疾病如糖尿病、肥胖等的關聯也是熱點。試劑盒檢測范圍為0.156 ng/mL-10 ng/mL，廣泛應用于代謝性疾病機制研究，如糖尿病、肥胖癥模型中的脂肪酸合成與分解動態分析，也可用于肝臟、肌肉等代謝活躍組織的能量代謝調控研究，為探索營養干預、基因調控對脂質代謝的影響提供可靠工具。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。

縮寫：

malonyl CoA

種屬：

Mus musculus (Mouse)

樣本類型：

serum, plasma, tissue homogenates

檢測范圍：

0.156 ng/mL-10 ng/mL

靈敏度：

0.039 ng/mL

反應時間：

1-5h

樣本體積：

50-100ul

檢測波長：

450 nm

研究領域：

Others

測定原理：

quantitative

測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse malonyl CoA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1000	Average %	90
	Range %	84-996
1:2000	Average %	95
	Range %	89-99
1:4000	Average %	99
	Range %	95-105
1:8000	Average %	98
	Range %	90-106

回收率：

The recovery of mouse malonyl CoA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	90	86-96
EDTA plasma (n=4)	95	87-99

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
10	2.214	2.298	2.256	2.159
5	1.791	1.765	1.778	1.681
2.5	1.224	1.268	1.246	1.149
1.25	0.842	0.812	0.827	0.730
0.625	0.572	0.547	0.560	0.463
0.312	0.381	0.365	0.373	0.276
0.156	0.201	0.209	0.205	0.108
0	0.096	0.098	0.097

本試劑盒所含材料：

A micro ELISA plate ---The 96-well plate has been pre-coated with an anti-Mouse malonyl CoA antibody.
Two vials lyophilized standard ---Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
One vial Biotin-labeled malonyl CoA antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.
One vial HRP-avidin (100 x concentrate) (120 μl/bottle) ---Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
One vial Biotin-antibody Diluent (15 ml/bottle) ---Dilute the Biotin-antibody.
One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the HRP-avidin solution.
One vial Sample Diluent (50 ml/bottle) ---Dilute the sample to an appropriate concentration.
One vial Wash Buffer (25 x concentrate) (20 ml/bottle) ---Wash away unbound or free substances.
One vial TMB Substrate (10 ml/bottle) ---Act as the chromogenic agent. TMB interacts with HRP, eliciting the solution turns blue.
One vial Stop Solution (10 ml/bottle) ---Stop the color reaction. The solution color immediately turns from blue to yellow.
Four Adhesive Strips (For 96 wells) ---Cover the microplate when incubating.
An instruction manual

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本試劑盒不含材料：

A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
An incubator can provide stable incubation conditions up to 37°C±5°
Centrifuge
Vortex
Squirt bottle, manifold dispenser, or automated microplate washer
Absorbent paper for blotting the microtiter plate
50-300ul multi-channel micropipette
Pipette tips
Single-channel micropipette with different ranges
100ml and 500ml graduated cylinders
Deionized or distilled water
Timer
Test tubes for dilution

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數據處理：

ELISA操作視頻及標曲繪制軟件下載

貨期：

3-5 working days

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.