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  4. Mouse macrophage inflammatory protein 3β,MIP-3β ELISA kit

Mouse macrophage inflammatory protein 3β,MIP-3β ELISA kit

  • 中文名稱：
    小鼠巨噬細(xì)胞炎性蛋白3β(MIP-3β/ELC/CCL19)酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-E04671m
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥3200/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    小鼠巨噬細(xì)胞炎性蛋白3β(MIP-3β/ELC/CCL19)酶聯(lián)免疫試劑盒（CSB-E04671m）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿樣本中的CCL19含量。CCL19，即趨化因子CCL19，是一種小分子蛋白，主要在樹突狀細(xì)胞、T細(xì)胞和多種腫瘤細(xì)胞上表達(dá)。它通過(guò)與其受體CCR7結(jié)合，在免疫系統(tǒng)中發(fā)揮重要作用，如趨化樹突狀細(xì)胞和T細(xì)胞浸潤(rùn)腫瘤，介導(dǎo)免疫細(xì)胞釋放細(xì)胞因子，抑制腫瘤增殖、遷移和侵襲，是腫瘤免疫治療的重要靶點(diǎn)。試劑盒檢測(cè)范圍為39 pg/ml- 2500 pg/ml，靈敏度為9.75 pg/ml。科研人員可通過(guò)開展免疫性疾病機(jī)制研究，如自身免疫模型、感染性疾病或腫瘤微環(huán)境相關(guān)實(shí)驗(yàn)，尤其適用于評(píng)估淋巴結(jié)、脾臟等免疫器官中趨化因子的調(diào)控規(guī)律；寬泛的線性范圍特別適用于動(dòng)態(tài)監(jiān)測(cè)炎癥模型中趨化因子的表達(dá)變化本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書。
  • 別名：
    Ccl19 ELISA Kit; Elc ELISA Kit; Scya19 ELISA Kit; C-C motif chemokine 19 ELISA Kit; Epstein-Barr virus-induced molecule 1 ligand chemokine ELISA Kit; EBI1 ligand chemokine ELISA Kit; ELC ELISA Kit; Small-inducible cytokine A19 ELISA Kit
  • 縮寫：
  • Uniprot No.：
  • 種屬：
    Mus musculus (Mouse)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測(cè)范圍：
    39 pg/ml - 2500 pg/ml
  • 靈敏度：
    9.75 pg/ml
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測(cè)波長(zhǎng)：
    450 nm
  • 研究領(lǐng)域：
    Immunology
  • 測(cè)定原理：
    quantitative
  • 測(cè)定方法：
    Sandwich
  • 精密度：

    Intra-assay Precision (Precision within an assay): CV%<8%

    Three samples of known concentration were tested twenty times on one plate to assess.

    Inter-assay Precision (Precision between assays): CV%<10%

    Three samples of known concentration were tested in twenty assays to assess.

  • 線性度：

    To assess the linearity of the assay, samples were spiked with high concentrations of mouse MIP-3β in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

    　

    Sample

    Serum(n=4)

    1:1

    Average %

    96

    Range %

    93-99

    1:2

    Average %

    104

    Range %

    100-107

    1:4

    Average %

    85

    Range %

    81-89

    1:8

    Average %

    98

    Range %

    94-103

  • 回收率：

    The recovery of mouse MIP-3β spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample Type

    Average % Recovery

    Range

    Serum (n=5)

    89

    85-94

    EDTA plasma (n=4)

    108

    104-112

  • 標(biāo)準(zhǔn)曲線：

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

    pg/ml

    OD1

    OD2

    Average

    Corrected

    2500

    2.830

    2.786

    2.808

    2.629

    1250

    2.286

    2.301

    2.294

    2.115

    625

    1.656

    1.672

    1.664

    1.485

    312.5

    1.007

    1.069

    1.038

    0.859

    156.25

    0.683

    0.698

    0.691

    0.512

    78

    0.457

    0.438

    0.448

    0.269

    39

    0.321

    0.331

    0.326

    0.147

    0

    0.178

    0.179

    0.179

    　

  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    Strongly chemotactic for naive (L-selectinhi) CD4 T-cells and for CD8 T-cells and weakly attractive for resting B-cells and memory (L-selectinlo) CD4 T-cells. May play a role in promoting encounters between recirculating T-cells and dendritic cells and in the migration of activated B-cells into the T-zone of secondary lymphoid tissues. Binds to chemokine receptor CCR7. Binds to atypical chemokine receptor ACKR4 and mediates the recruitment of beta-arrestin (ARRB1/2) to ACKR4.
  • 亞細(xì)胞定位：
    Secreted.
  • 蛋白家族：
    Intercrine beta (chemokine CC) family
  • 組織特異性：
    Highly expressed by dendritic cells in mesenteric and peripheral lymph nodes. Significant expression in spleen (T cell zone or periarteriolar lymphatic sheath) and Peyer patches. Low expression in thymus.
  • 數(shù)據(jù)庫(kù)鏈接：


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