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中文名稱:小鼠低密度脂蛋白膽固醇(LDL-C)酶聯免疫試劑盒
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貨號:CSB-EQ027860MO
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規格:96T/48T
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價格:¥3600/¥2500
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其他:
產品詳情
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產品描述:小鼠低密度脂蛋白膽固醇(LDL-C)酶聯免疫試劑盒(CSB-EQ027860MO)為競爭法ELISA試劑盒,定量檢測血清、血漿、組織勻漿樣本中的LDL-C含量。LDL-C(低密度脂蛋白膽固醇)是心血管疾病重要風險因素。其水平升高易在血管壁沉積形成斑塊。科研聚焦降低LDL-C水平以減少心血管事件。研究機制包括抑制合成、加速清除等,如開發他汀類、PCSK9抑制劑等藥物來干預該靶點。試劑盒檢測范圍為0.312 μg/mL-20 μg/mL,該產品可廣泛應用于小鼠模型中的脂質代謝機制研究、心血管疾病相關藥物開發、飲食干預實驗或基因工程動物模型的表型分析,為評估脂質代謝異常、動脈硬化病理進程及潛在治療策略提供可靠的實驗工具。本品僅用于科研,不用于臨床診斷,產品具體參數及操作步驟詳見產品說明書。
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縮寫:LDL-C
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種屬:Mus musculus (Mouse)
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樣本類型:serum, plasma, tissue homogenates
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檢測范圍:0.312 μg/mL-20 μg/mL
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靈敏度:0.078 μg/mL
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反應時間:1-5h
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樣本體積:50-100ul
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檢測波長:450 nm
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研究領域:Cardiovascular
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測定原理:quantitative
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測定方法:Competitive
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精密度:
Intra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess. -
線性度:
To assess the linearity of the assay, samples were spiked with high concentrations of mouse LDL-C in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. Sample Serum(n=4) 1:100 Average % 86 Range % 82-92 1:200 Average % 94 Range % 91-98 1:400 Average % 107 Range % 103-114 1:800 Average % 89 Range % 85-94 -
回收率:
The recovery of mouse LDL-Cspiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 88 84-93 EDTA plasma (n=4) 91 87-95 -
標準曲線:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. μg/ml OD1 OD2 Average 20 0.105 0.107 0.106 10 0.173 0.178 0.176 5 0.248 0.255 0.252 2.5 0.330 0.345 0.338 1.25 0.430 0.443 0.437 0.625 0.640 0.682 0.661 0.312 0.941 1.011 0.976 0 1.752 1.834 1.793 -
數據處理:
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貨期:3-5 working days
引用文獻
- Parabacteroides distasonis ameliorates insulin resistance via activation of intestinal GPR109a Y Sun,Nature communications,2023
- Phytosterols Alleviate Hyperlipidemia by Regulating Gut Microbiota and Cholesterol Metabolism in Mice WJ Lv,Oxidative medicine and cellular longevity,2023
- Involvement of pyroptosis pathway in epicardial adipose tissue-myocardium axis in experimental heart failure with preserved ejection fraction YY Xia,Biochemical and biophysical research communications,2022
- Regulation of nicotinic acetylcholine receptor α3 subtype in adipose tissue dysfunction Bai X, et al,Prostaglandins & Other Lipid Mediators,2019
- Han.et al,Experimental and Therapeutic Medicine,2018
- Mingnuan Han.et al,Experimental and Therapeutic Medicine,2018
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