在线日韩日本国产亚洲丨少妇伦子伦情品无吗丨欧美性猛交xxxx免费看蜜桃丨精品人妻系列无码一区二区三区丨亚洲精品无码不卡在线播放

Your Good Partner in Biology Research

  1. 首頁
  2. 試劑盒
  3. 酶聯免疫試劑盒
  4. Mouse lipoprotein-associated phospholipase A2,Lp-PLA2 ELISA Kit

Mouse lipoprotein-associated phospholipase A2,Lp-PLA2 ELISA Kit

  • 中文名稱：
    小鼠脂蛋白相關磷脂酶A2(Lp-PLA2)酶聯免疫試劑盒
  • 貨號：
    CSB-E08321m
  • 規格：
    96T/48T
  • 價格：
    ￥3800/￥2500
  • 其他：

產品詳情

  • 產品描述：
    小鼠脂蛋白相關磷脂酶A2(Lp-PLA2)酶聯免疫試劑盒（CSB-E08321m）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織培養上清液、組織勻漿樣本中的PLA2G7含量。PLA2G7即血小板活化因子乙酰水解酶，它能水解血小板活化因子。該靶點與炎癥、心血管疾病等密切相關。研究機制方面主要聚焦于其在炎癥信號傳導中的作用，以及對脂質代謝的影響，探索其抑制劑有望為相關疾病治療提供新策略。試劑盒檢測范圍為7.8 pg/mL-500 pg/mL，可在科研中用于評估小鼠模型心血管疾病進程、炎癥相關機制研究或藥物干預效果分析。為科研人員提供了一種高靈敏度的工具，可廣泛用于基礎醫學研究、藥效評價及分子機制探索等領域。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
  • 別名：
    Pla2g7 ELISA Kit; Pafah ELISA Kit; Platelet-activating factor acetylhydrolase ELISA Kit; PAF acetylhydrolase ELISA Kit; EC 3.1.1.47 ELISA Kit; 1-alkyl-2-acetylglycerophosphocholine esterase ELISA Kit; 2-acetyl-1-alkylglycerophosphocholine esterase ELISA Kit; LDL-associated phospholipase A2 ELISA Kit; LDL-PLA(2) ELISA Kit; PAF 2-acylhydrolase ELISA Kit
  • 縮寫：
    PLA2G7
  • Uniprot No.：
  • 種屬：
    Mus musculus (Mouse)
  • 樣本類型：
    serum, plasma, cell culture supernates, tissue homogenates
  • 檢測范圍：
    7.8 pg/mL-500 pg/mL
  • 靈敏度：
    1.95 pg/mL
  • 反應時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領域：
    Metabolism
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse Lp-PLA2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)  
    1:100 Average % 92  
    Range % 87-102  
    1:200 Average % 95  
    Range % 84-107  
    1:400 Average % 95  
    Range % 83-102  
    1:800 Average % 96  
    Range % 84-105  
  • 回收率：
    The recovery of mouse Lp-PLA2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 97 86-105  
    EDTA plasma (n=4) 96 88-109  
                 
                 
  • 標準曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average Corrected  
    500 2.715 2.652 2.684 2.565  
    250 1.817 1.913 1.865 1.746  
    125 1.113 1.165 1.139 1.020  
    62.5 0.600 0.627 0.614 0.495  
    31.2 0.380 0.393 0.387 0.268  
    15.6 0.273 0.292 0.283 0.164  
    7.8 0.192 0.202 0.197 0.078  
    0 0.116 0.122 0.119    
  • 數據處理：
  • 貨期：
    3-5 working days

產品評價

靶點詳情

  • 功能：
    Lipoprotein-associated calcium-independent phospholipase A2 involved in phospholipid catabolism during inflammatory and oxidative stress response. At the lipid-aqueous interface, hydrolyzes the ester bond of fatty acyl group attached at sn-2 position of phospholipids (phospholipase A2 activity). Specifically targets phospholipids with a short-chain fatty acyl group at sn-2 position. Can hydrolyze phospholipids with long fatty acyl chains, only if they carry oxidized functional groups. Hydrolyzes and inactivates platelet-activating factor (PAF, 1-O-alkyl-2-acetyl-sn-glycero-3-phosphocholine), a potent proinflammatory signaling lipid that acts through PTAFR on various innate immune cells. Hydrolyzes oxidatively truncated phospholipids carrying an aldehyde group at omega position, preventing their accumulation in lipoprotein particles and uncontrolled proinflammatory effects. As part of high-density lipoprotein (HDL) particles, can hydrolyze phospholipids having long-chain fatty acyl hydroperoxides at sn-2 position and protect against potential accumulation of these oxylipins in the vascular wall. Catalyzes the release from membrane phospholipids of F2-isoprostanes, lipid biomarkers of cellular oxidative damage.
  • 基因功能參考文獻：
    1. Resveratrol downregulates Lp-PLA2 expression by inhibiting oxidative stress via SIRT1 pathway in a mouse model of chronic inflammation. PMID: 28608449
    2. Lp-PLA2 augments the inflammatory response after MI and antagonizes healing by disrupting the balance between inflammation and repair. PMID: 26232205
    3. Plg from mouse plasma contains oxPtdPC adducts that are not affected by the action of Lp-PLA(2), suggesting that linkage to Plg protects oxPtdPCs from metabolism during their transport in the plasma. PMID: 21614211
    4. SAA up-regulates Lp-PLA2 production significantly via a FPRL1/MAPKs./PPAR-gamma signaling pathway. PMID: 23623642
    5. Deletion of Pla2g7 decreases small intestinal polyp and colon tumor incidence in ApcMin/+ mice. PMID: 23361301
    6. Pla2g7 and Tnfrsf21 have been identified as genetic susceptibility to influenza genes in mice. PMID: 22427645
    7. Pla2g7 plays a crucial physiological role in smooth muscle cell differentiation from stem cells, and interactions between Nrf3 and Pla2g7 are essential. PMID: 22247257
    8. The effects of a specific lp-PLA2 inhibitor on atherosclerosis in ApoE-deficient mice and its associated mechanisms, are reported. PMID: 21909350
    9. Studies designed to evaluate the ability of precursor forms of PAF-AH to mature to fully active proteins indicated that the N-terminal end of human and mouse PAF-AH played important and opposite roles in this process. PMID: 20331434
    10. PAF acetylhydrolase activity in the uterus in early pregnancy was not produced locally but probably resulted from the influx of the plasma form of the enzyme PMID: 11833932
    11. deletion of both subunits induces a marked loss of germ cells at an early spermatogenic stage. PMID: 12551946
    12. PAF acetylhydrolases play key roles in the hydrolysis of F2-isoprostanes esterified on phospholipids in vivo PMID: 16371369
    13. over-expression of the PAF-AH (I) catalytic subunits induces centrosomal amplification and microtubule disorganization by disturbing intracellular localization of LIS1 PMID: 17903175
    14. inactivation of PAF, produced by TEC, by the overexpression of plasma PAF-AH affects survival, migration, and the angiogenic response of TEC both in vitro and in vivo PMID: 17908960
    15. mouse and human PAF-AHs associate with human HDL particles of different density PMID: 18434304

    顯示更多

    收起更多

  • 亞細胞定位：
    Secreted, extracellular space.
  • 蛋白家族：
    AB hydrolase superfamily, Lipase family
  • 組織特異性：
    Plasma.
  • 數據庫鏈接：

    KEGG: mmu:27226

    STRING: 10090.ENSMUSP00000024706

    UniGene: Mm.9277



主站蜘蛛池模板： 日本边添边摸边做边爱的网站 | 巨爆乳无码视频在线观看| 丰满大爆乳波霸奶| 天天摸天天做天天爽天天弄| 日韩人妻少妇一区二区三区| 国产欧美一区二区三区免费视频 | 日本特黄特色aaa大片免费| 99精品久久99久久久久| 久久久欧美精品激情| 国产三级视频在线观看视| 久久久久久人妻无码| 亚洲精品午夜理伦不卡在线观看| 日本三级理论久久人妻电影| 国产大屁股喷水视频在线观看 | 黑人巨大精品欧美一区二区免费| 国偷自产一区二区三区在线视频| 99re6热精品视频在线观看| 色情一区二区三区免费看| 香蕉久久精品日日躁夜夜躁夏| 97无码免费人妻超级碰碰碰碰| 亚洲乱码日产一区三区| 18精品久久久无码午夜福利| 国产精品国产亚洲精品看不卡| 99久久久无码国产精品免费砚床| 内射小寡妇无码| 一区二区三区四区在线 | 中国| 红桃av一区二区三区在线无码av| 国产精品婷婷久久爽一下| 欧美黑人又粗又大的性格特点| 国产乱人伦偷精品视频aaa| 少妇午夜福利一区二区| 亚洲中文字幕无码爆乳app| 又色又爽又黄的视频软件app| 国产真人做爰视频免费| 少妇无码av无码去区钱| 在线看片免费人成视频影院看| 亚洲综合色区无码专区| 国产精品熟妇视频国产偷人| 欧美va天堂在线电影| 国产性生交xxxxx无码| 国产免费二卡3卡四卡|